CD4<sup>+</sup>T Helper Cell Responses against Human Bocavirus Viral Protein 2 Viruslike Particles in Healthy Adults

Lindner, Juha; Zehentmeier, Sandra; Franssila, Rauli; Barabas, Sascha; Schroeder, Josef; Deml, Ludwig; Modrow, Susanne

doi:10.1086/592985

Cited by 31 publications

(43 citation statements)

References 44 publications

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“…PCR-1 is frequently performed in cases of aplastic anemia or engraftment failure in stem cell transplant patients, but we never found PCR positivity in the peripheral blood of patients who were seropositive before transplantation (unpublished data). Also, a recent study showed no differences in B19 T-cell immunity when patients assigned to a group with "B19 DNApositive myocarditis" were compared to seropositive healthy controls (18).…”

Section: Resultsmentioning

confidence: 95%

High Prevalence of Human Parvovirus B19 DNA in Myocardial Autopsy Samples from Subjects without Myocarditis or Dilative Cardiomyopathy

Enders²,

et al. 2009

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Human parvovirus B19 has been linked to a variety of cardiac diseases, as well as to erythema infectiosum, acute arthropathy, and fetal hydrops. A causal association between viral infection and cardiac disease was frequently postulated following the detection of B19 DNA by PCR in endomyocardial biopsy specimens. Since the lifelong persistence of B19 DNA in bone marrow, skin, synovia, tonsils, and liver was previously reported, the aim of our study was to investigate the possibility of asymptomatic B19 DNA persistence in heart tissue. Myocardial autopsy and postmortem blood samples were prospectively collected from 69 bodies sent to the Department of Forensic Medicine, Freiburg University Medical Center, for inquests. All study subjects were screened for B19-specific antibodies using a commercial enzyme immunoassay. Tissue samples were analyzed by real-time PCR for the presence of viral DNA. Since the presence of B19 genotype 2, known to have been circulating before 1960, would prove long-lasting persistence, the presence of the B19 genotype was retrospectively determined in seven of the study subjects by melting temperature analysis and sequencing of the PCR product. B19 DNA was found in myocardial samples from 46 of 48 seropositive and in none of 21 seronegative individuals. B19 genotype 1 was found in three patients born between 1950 and 1969. Genotype 2 was found in four patients born between 1927 and 1957. Our findings suggest lifelong persistence of B19 DNA in heart tissue. Thus, the detection of B19 DNA in myocardial biopsy specimens alone is not sufficient to postulate a relationship between B19 infection and cardiac disease.

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Section: Resultsmentioning

confidence: 95%

High Prevalence of Human Parvovirus B19 DNA in Myocardial Autopsy Samples from Subjects without Myocarditis or Dilative Cardiomyopathy

Enders²,

et al. 2009

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“…However, analysis of more than 100 amniotic fluid samples from pregnant women with hydrops fetalis caused by unknown reasons did not reveal detectable amounts of HBoV genomes. Yet, as primary HBoV infections predominantly occur in young children and are rare in adults due to the presence of an ubiquitous virus-specific immunity [ 23,54 ], a potential effect of acute HBoV infections on pregnancy cannot be entirely excluded.…”

Section: Clinical Manifestations Of Hbov Infectionmentioning

confidence: 99%

“…Thereby, frequent interferon-␥ -mediated CD4+ T helper cell reactions were observed against HBoV VLP consisting of VP2 proteins [23] . Similar data have been previously found for B19V-specific cellular immune responses [62][63][64][65] .…”

Section: Immune Responses Against Hbovmentioning

confidence: 99%

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Human Bocavirus – A Novel Parvovirus to Infect Humans

Lindner

Modrow²

2008

Intervirology

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For almost three decades parvovirus B19 has been described as the only member of the Parvoviridae to infect and cause illness in humans. This statement was correct until 2005 when a group of Swedish scientists identified a previously uncharacterized virus in pools of human nasopharyngeal aspirates obtained from individuals suffering from diseases of the respiratory tract. Comprehensive sequence and phylogenetic analysis allowed the identification of the new virus as a member of the Parvoviridae. Based on its close relation to the minute virus of canines and the bovine parvovirus, it was named human bocavirus (HBoV). Since the identification of HBoV, viral genomes have been frequently detected worldwide in nasopharyngeal swabs, serum and fecal samples almost exclusively derived from young children with various symptoms of the respiratory or the gastrointestinal tract. The detection of HBoV genomes tends to be associated with elevated rates of coinfections with further respiratory viruses, e.g. respiratory syncytial virus or metapneumovirus. First studies on virus-specific immune responses have described the presence of ubiquitous humoral and cellular immune reactions against HBoV in adults and adolescents, indicating a high seroprevalence of this new virus in humans.

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“…[1][2][3][4][5][6][7][8][9][10] The infection is systemic and viremic, and elicits B-and T-cell responses. [11][12][13][14][15][16][17] HBoV is often found by PCR in the nasopharynx of children <3 years of age with upper or lower respiratory tract symptoms, both with and without other co-infecting viruses. 1,9,[18][19][20][21][22] HBoV found in stools of children with gastroenteritis, with uncertain clinical significance.…”

Section: Introductionmentioning

confidence: 99%

Absence of human bocavirus from deceased fetuses and their mothers

Riipinen

Väisänen

Lahtinen

et al. 2010

Journal of Clinical Virology

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CD4⁺T Helper Cell Responses against Human Bocavirus Viral Protein 2 Viruslike Particles in Healthy Adults

Abstract: Data suggest the presence of HBoV-specific immune responses in adults and strongly support a high prevalence of HBoV among humans.

Cited by 31 publications

References 44 publications

High Prevalence of Human Parvovirus B19 DNA in Myocardial Autopsy Samples from Subjects without Myocarditis or Dilative Cardiomyopathy

High Prevalence of Human Parvovirus B19 DNA in Myocardial Autopsy Samples from Subjects without Myocarditis or Dilative Cardiomyopathy

Human Bocavirus – A Novel Parvovirus to Infect Humans

Absence of human bocavirus from deceased fetuses and their mothers

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CD4+T Helper Cell Responses against Human Bocavirus Viral Protein 2 Viruslike Particles in Healthy Adults

Abstract: Data suggest the presence of HBoV-specific immune responses in adults and strongly support a high prevalence of HBoV among humans.

Cited by 31 publications

References 44 publications

High Prevalence of Human Parvovirus B19 DNA in Myocardial Autopsy Samples from Subjects without Myocarditis or Dilative Cardiomyopathy

High Prevalence of Human Parvovirus B19 DNA in Myocardial Autopsy Samples from Subjects without Myocarditis or Dilative Cardiomyopathy

Human Bocavirus – A Novel Parvovirus to Infect Humans

Absence of human bocavirus from deceased fetuses and their mothers

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CD4⁺T Helper Cell Responses against Human Bocavirus Viral Protein 2 Viruslike Particles in Healthy Adults