CD45 in ocular tissues during larval and juvenile stages and early stages of V. anguillarum infection in young lumpfish (Cyclopterus lumpus)

Gendron, Robert L.; Hyde, Tatiana; Paradis, Hélène; Cao, Trung; Machimbirike, Vimbai Irene; Segovia, Cristopher; Vásquez, Ignacio; Ghasemieshkaftaki, Maryam; Scapigliati, Giuseppe; Boyce, Danny; Santander, Javier

doi:10.1016/j.fsi.2022.08.023

Cited by 5 publications

(14 citation statements)

References 53 publications

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“…Western blotting for spotted wolffish PCNA, CD45, Sox9 and Tubulin further validated the utility of the panel of anti-teleost and/or anti-mammalian monoclonal antibody reagents we used to obtain the results on spotted wolffish tissues herein. Although there were differences in the molecular weights of spotted wolffish versus lumpfish anti-CD45 immunoreactive bands on western blotting, these differences could be due to either species specific differential splicing or posttranslational modifications as we have described previously for sea bass versus lumpfish CD45 and as others have described in other species 4,45 . The Basic Local Alignment Search Tool at National Center for Biotechnology Information (BLAST) indicated 94.8% and 90.7% protein sequence identities for seabass CD45 and Sox9 compared to the lumpfish and sablefish (Anoploploma fibria) genomes, respectively, which were used as a proxies for spotted wolffish in the absence of spotted wolfish genomic information.…”

Section: Discussionmentioning

confidence: 53%

“…We described previously that the CD45 phosphatase domains are very conserved between taxa. Although the R0 and ABC domains are not typically conserved between species and are in different fish clusters between lumpfish and seabass, there are still similarities in this domain between lumpfish and seabass which supports antibody cross reaction with spotted wolffish 4 . Finally, our IHC validation experiments clearly show DLT22 antibody anti-CD45 staining in spotted wolffish head kidney tissue where one would expect such staining in the significant immune lineage parenchymal cell component in this primary lymphoid organ but not in spotted wolffish muscle tissue where one would not expect any appreciable immune lineage cell component in the parenchymal muscle tissue (Supplemental Fig.…”

Section: Discussionmentioning

confidence: 97%

“…Our work nonetheless provides a basis to further explore the visual system of spotted wolffish, which are designated a threatened species in Canada. In previous work, we characterized the visual system of the North Atlantic lumpfish [1][2][3][4][5][6][7] . The work herein on spotted wolffish suggests some differences (scleral cartilage thickness, shape and reactivity) and similarities (expression of cellular and immune markers PCNA, IgM and CD45), between the two species.…”

Section: Discussionmentioning

confidence: 99%

“…We have recently characterized the visual system of the North Atlantic lumpfish (Cyclopterus lumpus) 1 . The characteristics we have observed in the eyes of this species and their importance as models of ocular and immune disorders [2][3][4][5][6][7] has prompted comparative analyses toward exploring the visual systems of other locally available North Atlantic marine teleosts as described in the work herein on spotted wolffish.…”

mentioning

confidence: 99%

“…reported under stressful conditions 14,20 . Our previous work has described the morphological and molecular changes in ocular tissues of lumpfish experimentally challenged intraperitoneally with the marine pathogen V. anguillarum 4,6 but studies of the response of spotted wolffish eyes to exogenous insults have not been reported.…”

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confidence: 99%

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Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish

Kwabiah,

Weiland,

Henderson

et al. 2024

Sci Rep

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Adult vertebrate cartilage is usually quiescent. Some vertebrates possess ocular scleral skeletons composed of cartilage or bone. The morphological characteristics of the spotted wolffish (Anarhichas minor) scleral skeleton have not been described. Here we assessed the scleral skeletons of cultured spotted wolffish, a globally threatened marine species. The healthy spotted wolffish we assessed had scleral skeletons with a low percentage of cells staining for the chondrogenesis marker sex-determining region Y-box (Sox) 9, but harboured a population of intraocular cells that co-express immunoglobulin M (IgM) and Sox9. Scleral skeletons of spotted wolffish with grossly observable eye abnormalities displayed a high degree of perochondrial activation as evidenced by cellular morphology and expression of proliferating cell nuclear antigen (PCNA) and phosphotyrosine. Cells staining for cluster of differentiation (CD) 45 and IgM accumulated around sites of active chondrogenesis, which contained cells that strongly expressed Sox9. The level of scleral chondrogenesis and the numbers of scleral cartilage PCNA positive cells increased with the temperature of the water in which spotted wolffish were cultured. Our results provide new knowledge of differing Sox9 spatial tissue expression patterns during chondrogenesis in normal control and ocular insult paradigms. Our work also provides evidence that spotted wolffish possess an inherent scleral chondrogenesis response that may be sensitive to temperature. This work also advances the fundamental knowledge of teleost ocular skeletal systems.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish

Kwabiah,

Weiland,

Henderson

et al. 2024

Sci Rep

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Systematic analysis of ocular features and responses of cultured spotted wolffish (Anarhichas minor)

Murphy,

Kwabiah,

Rouah

et al. 2024

Journal of Fish Diseases

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A better understanding of unique anatomical and functional features of the visual systems of teleost fish could provide key knowledge on how these systems influence the health and survival of these animals in both wild and culture environments. We took a systematic approach to assess some of the visual systems of spotted wolffish (Anarhichas minor), a species of increasing importance in North Atlantic aquaculture initiatives. The lumpfish (Cyclopterus lumpus) was included in these studies in a comparative manner to provide reference. Histology, light and electron microscopy were used to study the spatial distribution and occurrence of cone photoreceptor cells and the nature of the retinal tissues, while immunohistochemistry was used to explore the expression patterns of two photoreceptor markers, XAP‐1 and XAP‐2, in both species. A marine bacterial infection paradigm in lumpfish was used to assess how host–pathogen responses might impact the expression of these photoreceptor markers in these animals. We define a basic photoreceptor mosaic and present an ultrastructural to macroscopic geographical configuration of the retinal pigment tissues in both animals. Photoreceptor markers XAP‐1 and XAP‐2 have novel distribution patterns in spotted wolffish and lumpfish retinas, and exogenous pathogenic influences can affect the normal expression pattern of XAP‐1 in lumpfish. Live tank‐side ophthalmoscopy and spectral domain optical coherence tomography (SD‐OCT) revealed that normal cultured spotted wolffish display novel variations in the shape of the retinal tissue. These two complementary imaging findings suggest that spotted wolffish harbour unique ocular features not yet described in marine teleosts and that visual function might involve specific retinal tissue shape dynamics in these animals. Finally, extensive endogenous biofluorescence is present in the retinal tissues of both animals, which raises questions about how these animals might use retinal tissue in novel ways for visual perception and/or communication. This work advances fundamental knowledge on the visual systems of two economically important but now threatened North Atlantic teleosts and provides a basic foundation for further research on the visual systems of these animals in health versus disease settings. This work could also be useful for understanding and optimizing the health and welfare of lumpfish and spotted wolffish in aquaculture towards a one health or integrative perspective.

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Novel Application of Non‐Invasive Methodological Approaches in Biomedical Sciences Towards Better Understanding of Marine Teleost Ocular Health and Disease

Gendron,

Kwabiah,

Paradis

et al. 2024

Journal of Fish Diseases

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Seafood is an important resource for global nutrition and food security, with both land and marine aquaculture playing pivotal roles. High visual acuity is key for health and survival of farmed, cultured, and wild fish. Cleaner fish technology to control parasite infestation has become important in marine aquaculture and highlights the importance of visual acuity in the efficacy of cleaner fish species. New clinical diagnostic approaches towards understanding and optimising fish visual health could benefit both aquacultured and wild fish populations. Opportunities for developing and using advanced non‐invasive clinical assessment and diagnosis of ocular health in wild, cultured, and experimental fish are key to more rapidly realising how threats to eye health in these animals might be better understood and mitigated. Ophthalmoscopy can rapidly and non‐invasively image anatomical aspects of retinal and anterior ocular tissues and has been used in mammalian biomedicine since the turn of the 20th century. More now than ever, labour‐intensive post‐mortem approaches for ocular analysis such as histology are increasingly being replaced or supplemented by application of various forms of optical coherence tomography (OCT) imaging of ocular tissues in mammalian biomedicine. Advances and availability of other methodological approaches such as three‐dimensional printing and computer science make instrument customisation affordable and adaptable. This review article will outline how ophthalmoscopy, OCT, and other methodologies are being applied towards understanding ocular health in teleost fish species and will describe some of the future opportunities that technological advances might afford in advancing ocular imaging in fish health and disease in general.

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CD45 in ocular tissues during larval and juvenile stages and early stages of V. anguillarum infection in young lumpfish (Cyclopterus lumpus)

Cited by 5 publications

References 53 publications

Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish

Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish

Systematic analysis of ocular features and responses of cultured spotted wolffish (Anarhichas minor)

Novel Application of Non‐Invasive Methodological Approaches in Biomedical Sciences Towards Better Understanding of Marine Teleost Ocular Health and Disease

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