CD63 expression by flow cytometry in the <i>in vitro </i>diagnosis of allergy to omeprazole

Gamboa, P. M.; Sánz, M L; Urrutia, I.; Jáuregui, I; Antépara, I.; Diéguez, I; Weck, A.L. de

doi:10.1034/j.1398-9995.2003.00155.x

Cited by 32 publications

(27 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It raises the sensitivity for the 4 NSAIDs to 76.1% at the expense of a marked decrease in specificity. This contrasts sharply with the observations made in betalactam allergy [49] and metamizol allergy [52,] in which the addition of the CAST to the FAST improved the sensitivity from 59 to 76 and 42 to 76%, respectively, without really impairing the specificity. While in IgE-mediated allergies, the correlation between flowcytometric basophil activation and sLT release is rather high (fig.…”

Section: Discussioncontrasting

confidence: 55%

See 1 more Smart Citation

A New Combined Test with Flowcytometric Basophil Activation and Determination of Sulfidoleukotrienes Is Useful for in vitro Diagnosis of Hypersensitivity to Aspirin and other Nonsteroidal Anti-Inflammatory Drugs

Sánz

Gamboa

Weck

2005

Int Arch Allergy Immunol

Self Cite

105

View full text Add to dashboard Cite

Background: We assessed whether nonsteroidal anti-inflammatory drugs (NSAIDs) may provoke blood basophil activation in vitro in aspirin- and NSAID-hypersensitive patients, as detected by a flowcytometric technique using the CD63 marker – flowcytometric basophil activation test (FAST) assay – in addition to the sulfidoleukotriene (sLT) release – the cellular allergen stimulation test (CAST). Methods: Sixty aspirin- and/or NSAID-hypersensitive patients were studied. Thirty control patients without history and negative provocation challenge were also included. The percentage of activated basophils after in vitro stimulation with NSAIDs at 3 different concentrations was evaluated by an anti-CD63 phycoerythrin conjugate (FAST assay) and the amount of sLTs released in the cell supernatant by ELISA (CAST assay). Results: For aspirin, the FAST indicated a sensitivity of 41.7%, a specificity of 100%, a positive predictive value of 100% and a negative predictive value of 99.4%; for paracetamol 11.7 and 100%, for metamizol 15 and 100%, for diclofenac 43.3 and 93.3%, and for naproxen 54.8 and 74.1%. Many patients showed positive tests to more than 1 NSAID. When considering the first 4 NSAIDs, the global sensitivity increased to 66.7%, while the specificity remained at 93.3%. The addition of the CAST results still increased the sensitivity up to 73.3%, but with a decrease of the specificity to 71.4%. Conclusions: The FAST shows a high percentage of positive reactions, which may reach 60–70% when 4 NSAIDs are tested and even 88% when the test is performed within 1 month of the last clinical drug exposure and reaction. The test has a high specificity above 90%. The addition of sLT determinations yields additional information in a few isolated cases. It is suggested that this test, when properly used, may help avoid some cumbersome and dangerous provocation challenges.

show abstract

Section: Discussioncontrasting

confidence: 55%

“…Furthermore, several studies have been published in the past few years with drugs such as betalactams [48, 49], myorelaxants [50, 51] and metamizol [52.] Some general reviews of the state of the art are available [53, 54].…”

Section: Discussionmentioning

confidence: 99%

A New Combined Test with Flowcytometric Basophil Activation and Determination of Sulfidoleukotrienes Is Useful for in vitro Diagnosis of Hypersensitivity to Aspirin and other Nonsteroidal Anti-Inflammatory Drugs

Sánz

Gamboa

Weck

2005

Int Arch Allergy Immunol

Self Cite

105

View full text Add to dashboard Cite

show abstract

“…It has also recently been claimed that diclofenac causes microscopic basophil degranulation but no CD63 expression [107]. Since these BAT-negative results have been obtained with drug concentrations 8–30 times lower than those found required for a positive BAT [97, 99], these results should not be considered relevant.…”

Section: Clinical Indications Of Batmentioning

confidence: 72%

“…In IgE-mediated allergies to single analgesics, e.g. pyrazolones, metamizol has attracted particular interest [99]. These patients differ from those with the NSAID hypersensitivity syndrome: they react specifically to a single drug or to a group of drugs of similar immunochemical structure.…”

Section: Clinical Indications Of Batmentioning

confidence: 99%

“…Many of these studies have been reviewed recently [8,9,10]. Herewith, tables 1, 2, 3, 4, 5, 6, 7summarize the results of validated clinical studies performed with various allergens such as inhalant allergens [25,26,27,28,29,30,31,32,33] (table 1), hymenoptera venoms [34,35,36,37,38,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,54,55,56] (table 2), latex [57,58,59,60,61,62,63] (table 3), food [64,65,66,67,68,69,70,71,72,73,74,75,76,77,78,79,80] (table 4) and drugs [81,82,83,84,85,86,87,88,89,90,91,92,93,94,95,96,97,98,99,100,101,…”

Section: Clinical Issues In the Diagnostic Use Of Batmentioning

confidence: 99%

See 1 more Smart Citation

Diagnostic Tests Based on Human Basophils: More Potentials and Perspectives than Pitfalls

Weck

Sánz

Gamboa

et al. 2008

Int Arch Allergy Immunol

Self Cite

171

178

View full text Add to dashboard Cite

For the diagnosis of allergy, cellular basophil activation tests (BAT), e.g. histamine or sulfidoleukotriene release tests, have long been introduced, but the expression of basophil activation markers such as CD63 and CD203c detected by flow cytometry has attracted more recent attention. A recent opinion paper in this Journal has stressed not only the potential but also the possible pitfalls of flow-cytometric BAT. We have applied clinical validation of various BAT in various ways for several years, and our experience shows that these new technologies have more potentials and perspectives than pitfalls. A comprehensive review of clinically validated studies on allergy to aeroallergens, insect venoms, latex, food allergens and drugs, e.g. myorelaxants, β-lactams, pyrazolones and non-steroidal anti-inflammatory drugs, as well as chronic urticaria shows clearly that even with different protocols, reproducible and meaningful results can be obtained. Although the available technologies may still be optimized and better standardized, there are no serious reasons to deprive allergic patients of clinically indicated BAT, which can be performed reliably by any laboratory with allergy and flow-cytometric capacity and expertise.

show abstract

Current awareness: Pharmacoepidemiology and drug safety

2003

Pharmacoepidemiology and Drug

View full text Add to dashboard Cite

In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of pharmacoepidemiology and drug safety. Each bibliography is divided into 19 sections: 1 Books, Reviews & Symposia; 2 General; 3 Anti‐infective Agents; 4 Cardiovascular System Agents; 5 CNS Depressive Agents; 6 Non‐steroidal Anti‐inflammatory Agents; 7 CNS Agents; 8 Anti‐neoplastic Agents; 9 Haematological Agents; 10 Neuroregulator‐Blocking Agents; 11 Dermatological Agents; 12 Immunosuppressive Agents; 13 Autonomic Agents; 14 Respiratory System Agents; 15 Neuromuscular Agents; 16 Reproductive System Agents; 17 Gastrointestinal System Agents; 18 Anti‐inflammatory Agents ‐ Steroidal; 19 Others. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted.

show abstract

CD63 expression by flow cytometry in the in vitro diagnosis of allergy to omeprazole

Cited by 32 publications

References 6 publications

A New Combined Test with Flowcytometric Basophil Activation and Determination of Sulfidoleukotrienes Is Useful for in vitro Diagnosis of Hypersensitivity to Aspirin and other Nonsteroidal Anti-Inflammatory Drugs

A New Combined Test with Flowcytometric Basophil Activation and Determination of Sulfidoleukotrienes Is Useful for in vitro Diagnosis of Hypersensitivity to Aspirin and other Nonsteroidal Anti-Inflammatory Drugs

Diagnostic Tests Based on Human Basophils: More Potentials and Perspectives than Pitfalls

Current awareness: Pharmacoepidemiology and drug safety

Contact Info

Product

Resources

About