CD87 as a marker for terminal granulocytic maturation: Assessment of its expression during granulopoiesis

Abstract

Background: Understanding the normal surface maturation pattern of granulocytes is essential for the recognition of abnormal patterns, which in turn may be of diagnostic or pathogenetic significance in disorders such as myelodysplastic syndromes and inherited bone marrow failure disorders. CD87 plays a role in cellular interaction, cell migration, and inflammatory response. Surface expression of this antigen has not been adequately studied on bone marrow granulocytes, and the small number of previous studies has provided conflicting data.Methods: Bone marrow aspirates from 11 control subjects were studied by flow cytometry and a lysed whole blood technique to compare surface expression of CD87 on marrow granulocytes with those of CD11b, CD16, CD35, and CD10, which are expressed at the myelocyte, metamyelocyte, band, and segmented stage of neutrophilic development, respectively. Four sorting experiments of CD87 ؉ granulocytes were also performed.Results: Our study showed no statistical difference between surface expression of CD35 and CD87 (P > 0.3), whereas significant differences existed between CD87 and the other antibodies (P < 0.004). Sorting experiments showed that more than 80% of CD87 ؉ cells were bands and segmented neutrophils. Dual staining for CD87 and CD35 showed that most CD87 ؉ granulocytes coexpress CD35. Conclusions: CD87 is expressed on granulocytes at the band and segmented neutrophil stage of development and can be used to study normal and abnormal granulopoiesis. Cytometry Part B (Clin. Cytometry) 51B:9 -13, 2003.

Large-Scale Exome-wide Association Analysis Identifies Loci for White Blood Cell Traits and Pleiotropy with Immune-Mediated Diseases

“…UPAR, also known as CD87, is a differentiation antigen on cells of the myelomonocytic lineage and also an activation antigen on monocytes and T lymphocytes. 41,42 The deleterious coding variant of CD87 rs4760A>G (p.Leu272Pro) 36 is also a strong eQTL for CD87 expression in monocytes and whole blood (Table S13). In addition to its role in plasminogen activation and fibrinolysis, UPAR is involved in cell adhesion, migration, and chemotaxis and is a regulator of the uptake by macrophages of apoptotic neutrophils.…”

“…40,86 CD87 is expressed on various immune cells including neutrophils, monocytes, macrophages, T cells, and basophils, as well as endothelial cells and hepatocytes. 41,87 The cleaved soluble form of CD87 might have a role in hematopoietic stem/progenitor cell mobilization. 88 Somatic mutations in ASXL1 are associated with risk of myelodysplastic syndrome (MDS [MIM: 614286]), chronic myelomonocytic leukemia (CMML [MIM: 607785]), and idiopathic cytopenia of undetermined significance (ICUS).…”

Expression profiling of snoRNAs in normal hematopoiesis and AML

“…Samples were processed via ammonium-chloride-potassium red cell lysis, washed once in phosphate-buffered saline, and then stained for flow cytometry using the following antibodies: CD34-phycoerythrin (PE) (PE-pool, Beckman Coulter, IM1459U), CD14-allophycocyanin (BD Biosciences, clone M5E2), CD15-fluorescein isothiocyanate (BD Biosciences, clone HI98), CD16-PE (BD Biosciences, clone 3G8), CD33-allophycocyanin (eBioscience, clone WM-53), CD3-V450 (eBioscience, clone OKT3), and CD19-PE (BD Biosciences, clone HIB19). Defined hematopoietic cell populations that were sorted included: promyelocytes (CD14 17 and CD34…”

Quantitative analysis of the expression of glycosylphosphatidylinositol‐anchored proteins during the maturation of different hematopoietic cell compartments of normal bone marrow

“…at the myelocyte and band maturation stages, respectively (5,6); in turn CD66b and CD16 would be expressed at earlier stages of maturation, the highest expression of CD16 being observed on bands and mature neutrophils (7)(8)(9)(10)(11). CD14 expression would be restricted to the latest stages of the maturation of normal monocytic cells (12).…”