CD8α+CD11c+ Extracellular Vesicles in the Lungs Control Immune Homeostasis of the Respiratory Tract via TGF-β1 and IL-10

Wan, Shuang‐Shuang; Wang, Shoujie; Weng, Luxi; Zhang, Gensheng; Lin, Zifan; Fei, Xuefeng; Zhang, Fanghui; Yang, Fei; Wang, JianLi; Cai, Zhijian

doi:10.4049/jimmunol.1701447

Cited by 17 publications

(12 citation statements)

References 48 publications

(51 reference statements)

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“…Wan et al investigated the effects of EVs from healthy mouse lungs in regulation of the immune balance in the respiratory tract. They found that, among the subsets of EVs, CD8α+ CD11c+ EVs contain immunosuppressive cytokines TGF‐β1 and IL‐10.…”

Section: Resultsmentioning

confidence: 99%

Extracellular vesicles and asthma: A review of the literature

Sangaphunchai

Todd

Fairclough

2020

Clin Experimental Allergy

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Asthma is a chronic, recurrent and incurable allergy‐related respiratory disease characterized by inflammation, bronchial hyperresponsiveness and narrowing of the airways. Extracellular vesicles (EVs) are a universal feature of cellular function and can be detected in different bodily fluids. Recent evidence has shown the possibility of using EVs in understanding the pathogenesis of asthma, including their potential as diagnostic and therapeutic tools. Studies have reported that EVs released from key cells involved in asthma can induce priming and activation of other asthma‐associated cells. A literature review was conducted on all current research regarding the role and function of EVs in the pathogenesis of asthma via the PRISMA statement method. An electronic search was performed using EMBASE and PubMed through to November 2018. The EMBASE search returned 76 papers, while the PubMed search returned 211 papers. Following duplicate removal, titles and abstracts were screened for eligibility with a total of 34 studies included in the final qualitative analysis. The review found evidence of association between the presence of EVs and physiological changes characteristic of asthma, suggesting that EVs are involved in the pathogenesis, with the weight of evidence presently favouring deleterious effects of EVs in asthma. Numerous studies highlighted differences in exosomal contents between EVs of healthy and asthmatic individuals, which could be employed as potential diagnostic markers. In some circumstances, EVs were also found to be suppressive to disease, but more often promote inflammation and airway remodelling. In conclusion, EVs hold immense potential in understanding the pathophysiology of asthma, and as diagnostic and therapeutic markers. While more research is needed for definitive conclusions and their application in medical practice, the literature presented in this review should encourage further research and discovery within the field of EVs and asthma.

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Section: Resultsmentioning

confidence: 99%

Extracellular vesicles and asthma: A review of the literature

Sangaphunchai

Todd

Fairclough

2020

Clin Experimental Allergy

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“…The cochlea is surrounded by the otic vesicle that gradually ossi es and becomes rigid as the mouse ages, especially after P10, and this makes it di cult to dissect the basilar membrane for extracting cochlear tissue-derived sEVs. Some recent studies have used enzyme digestion for the purpose of maintaining the integrity of the cells as much as possible in order to extract EVs from fat, brain, and tumor tissues [35,[83][84][85][86], while other studies have ground the tissues as a necessary step for extracting EVs [85,[87][88][89]. Crescitelli et al showed that the digestive enzymes in the existing tissue extraction methods are ineffective for bone tissue, and the methods for this type of tissue need further optimization [90].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Jiang¹,

Ma²,

Han³

et al. 2021

Preprint

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The cochlea is an important sensory organ for both balance and sound perception, and the formation of the cochlea is a complex developmental process. The development of the mouse cochlea begins on embryonic day (E)9 and continues until postnatal day (P)21 when the hearing system is considered mature. Small extracellular vesicles (sEVs), with a diameter ranging from 30 nm to 200 nm, have been considered as a significant medium for information communication in both the processing of physiological and pathological. However, there are no studies exploring the role of sEVs in the development of the cochlea. Here, we isolated tissue-derived sEVs from the cochleae of FVB mice at P3, P7, P14, and P21 by ultracentrifugation. These sEVs were first characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Next, we used small RNA-seq and mass spectrometry to characterize the microRNA transcriptomes and proteomics of cochlear sEVs from mice at different ages. Many microRNAs and proteins were discovered to be related with inner ear development, anatomical structure development, and the auditory nervous system development. These results all suggest that sEVs exist in the cochlea and are likely to be essential for the normal development of the auditory system. Our findings provide many sEV microRNA and protein targets for future studies of the roles of cochlear sEVs.

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“…EVs isolated from the lungs of mice have also been shown to contain the anti-inflammatory cytokines TGFβ-1 and IL-10. These EVs likely originate from dendritic cells that can communicate with T cells to inhibit their proliferation in response to a specific antigen [ 122 ]. There is also evidence that immune cell-derived EVs are involved in tissue remodeling and wound repair after inflammation has resolved, which can be pathological in the development of fibrosis in the lungs.…”

Section: Evs In Inflammatory Lung Diseasesmentioning

confidence: 99%

Extracellular vesicles: mediators of intercellular communication in tissue injury and disease

et al. 2021

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Intercellular communication is a critical process that ensures cooperation between distinct cell types and maintains homeostasis. EVs, which were initially described as cellular debris and devoid of biological function, are now recognized as key components in cell–cell communication. EVs are known to carry multiple factors derived from their cell of origin, including cytokines and chemokines, active enzymes, metabolites, nucleic acids, and surface molecules, that can alter the behavior of recipient cells. Since the cargo of EVs reflects their parental cells, EVs from damaged and dysfunctional tissue environments offer an abundance of information toward elucidating the molecular mechanisms of various diseases and pathological conditions. In this review, we discuss the most recent findings regarding the role of EVs in the progression of cancer, metabolic disorders, and inflammatory lung diseases given the high prevalence of these conditions worldwide and the important role that intercellular communication between immune, parenchymal, and stromal cells plays in the development of these pathological states. We also consider the clinical applications of EVs, including the possibilities for their use as novel therapeutics.

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CD8α+CD11c+ Extracellular Vesicles in the Lungs Control Immune Homeostasis of the Respiratory Tract via TGF-β1 and IL-10

Cited by 17 publications

References 48 publications

Extracellular vesicles and asthma: A review of the literature

Extracellular vesicles and asthma: A review of the literature

Characterization of the microRNA Transcriptomes and Proteomics of Cochlea Tissue-derived Small Extracellular Vesicles From Different Age of Mice After Birth

Extracellular vesicles: mediators of intercellular communication in tissue injury and disease

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