CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development

Tepekoy, Filiz; Öztürk, Saffet; Sözen, Berna; Ozay, Recep S.; Akkoyunlu, Gökhan; Demır, Necdet

doi:10.1016/j.repbio.2015.10.004

Cited by 11 publications

(13 citation statements)

References 48 publications

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“…26 In the mouse, CD90 was dominantly expressed in somatic compartments of ovarian follicles, suggesting that CD90 might have different functions. 27 Finally, CD105 is highly expressed in germ cell compartments in the ovary, suggesting its expression might be associated with the germline stem cells from which oocytes originate. 27 The presence of germ stem cells (GSC) in the mammalian ovary has been discussed extensively in recent years.…”

Section: Discussionmentioning

confidence: 99%

“…27 Finally, CD105 is highly expressed in germ cell compartments in the ovary, suggesting its expression might be associated with the germline stem cells from which oocytes originate. 27 The presence of germ stem cells (GSC) in the mammalian ovary has been discussed extensively in recent years. It has been postulated (i) that functional GSC are present in the ovary of mammalian adults, 8,[15][16][17]35,36 (ii) that the cells found in mammalian adult ovaries may be bipotent and able to give rise to granulosa and GSC, 23 and (iii) that a population of very small embryonic-like cells are present in different locations of the organism, including the ovary.…”

Section: Discussionmentioning

confidence: 99%

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Mesenchymal‐like stem cells in canine ovary show high differentiation potential

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

et al. 2017

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“…The second analysed molecule, endoglin (CD105), is associated with the TGFB superfamily. CD105 is a cell-surface co-receptor for TGFB1 and TGFB3 isoforms [7,20]. It is a glycoprotein that is expressed in various cell types, with high expression identified in endothelial cells [21].…”

Section: Discussionmentioning

confidence: 99%

“…Endoglin (CD105, ENG) is a 180 kDa homodimeric transmembrane glycoprotein. Its expression was determined in the luteinizing granulosa cells of the human ovary after culture in the presence of leukaemia inhibiting factor (LIF) [6,7]. It is also known as a mesenchymal stem cells (MSCs) marker.…”

Section: Introductionmentioning

confidence: 99%

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Bryja

Pieńkowski

Stefańska

et al. 2020

Medical Journal of Cell Biology

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The human granulosa cells (GCs) surround the oocyte and form the ovarian follicle’s proper architecture.These sub-populations include mural granulosa cells, antral granulosa cells, and cumulus granulosa cells.Their main functions are to support the oocyte’s growth (cumulus granulosa cells) and estradiol production (mural granulosa cells). After ovulation, the granulosa cells transform into the luteal cells of the corpus luteum and produce progesterone.Our study investigated the expression profile of three genes: TGFB1, CD105, and FSP1 during a 7-day in vitro culture. The analysis was conducted using the RT-qPCR technique.Changes in the expression of CD105 and FSP1 could be observed during the 7-day in vitro culture. In the case of TGFB, the expression remained at a similar level, with no statistically significant differences observed.Running title: Expression of TGFB1, CD105 and FSP1 in granulosa cells

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“…Immunoflourescence stainings on the P 3 cells were performed as previously described [26]. The evaluation of the immunoflourescence stainings were accomplished by ImageJ program (National Institutes of Health, Bethesda, MD, USA) as described previously [49]. Briefly, corrected total cell fluorescence was determined through the integrated density, area and the mean gray value of the cells.…”

Section: Methodsmentioning

confidence: 99%

Reduction of Inflammation and Enhancement of Motility after Pancreatic Islet Derived Stem Cell Transplantation Following Spinal Cord Injury

Karaöz¹,

Tepekoy²,

Yılmaz³

et al. 2019

J Korean Neurosurg Soc

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ObjectiveSpinal cord injury (SCI) is a very serious health problem, usually caused by a trauma and accompanied by elevated levels of inflammation indicators. Stem cell-based therapy is promising some valuable strategies for its functional recovery. Nestin-positive progenitor and/or stem cells (SC) isolated from pancreatic islets (PI) show mesenchymal stem cell (MSC) characteristics. For this reason, we aimed to analyze the effects of rat pancreatic islet derived stem cell (rPI-SC) delivery on functional recovery, as well as the levels of inflammation factors following SCI. MethodsrPI-SCs were isolated, cultured and their MSC characteristics were determined through flow cytometry and immunofluorescence analysis. The experimental rat population was divided into three groups : 1) laminectomy & trauma, 2) laminectomy & trauma & phosphate-buffered saline (PBS), and 3) laminectomy+trauma+SCs. Green fluorescent protein (GFP) labelled rPI-SCs were transplanted into the injured rat spinal cord. Their motilities were evaluated with Basso, Beattie and Bresnahan (BBB) Score. After 4-weeks, spinal cord sections were analyzed for GFP labeled SCs and stained for vimentin, S100β, brain derived neurotrophic factor (BDNF), 2’,3’-cyclic-nucleotide 3'-phosphodiesterase (CNPase), vascular endothelial growth factor (VEGF) and proinflammatory (interleukin [IL]-6, transforming growth factor [TGF]-β, macrophage inflammatory protein [MIP]-2, myeloperoxidase [MPO]) and anti-inflammatory (IL-1 receptor antagonis) factors. ResultsrPI-SCs were revealed to display MSC characteristics and express neural and glial cell markers including BDNF, glial fibrillary acidic protein (GFAP), fibronectin, microtubule associated protein-2a,b (MAP2a,b), β3-tubulin and nestin as well as antiinflammatory prostaglandin E2 receptor, EP3. The BBB scores showed significant motor recovery in group 3. GFP-labelled cells were localized on the injury site. In addition, decreased proinflammatory factor levels and increased intensity of anti-inflammatory factors were determined. ConclusionTransplantation of PI-SCs might be an effective strategy to improve functional recovery following spinal cord trauma.

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CD90 and CD105 expression in the mouse ovary and testis at different stages of postnatal development

Cited by 11 publications

References 48 publications

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

Mesenchymal‐like stem cells in canine ovary show high differentiation potential

Analysis of TGFB1, CD105 and FSP1 expression in human granulosa cells during a 7-day primary in vitro culture

Reduction of Inflammation and Enhancement of Motility after Pancreatic Islet Derived Stem Cell Transplantation Following Spinal Cord Injury

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