CDC Inca yellow field pea

Warkentin, Thomas D.; Tar’an, Bunyamin; Banniza, Sabine; Vandenberg, Albert; Bett, Kirstin E.; Arganosa, Gene; Barlow, Brent; Ife, Scott; Horner, Jaret; DeSilva, Devini; Wagenhoffer, Stacey; Liu, Yong; Prado, Thiago Reis; Mikituk, Kevin

doi:10.1139/cjps-2017-0141

Cited by 4 publications

(4 citation statements)

References 2 publications

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“…Of the five P. sativum cultivars evaluated, CDC Amarillo had the greatest vDAO concentration. It is important to note that CDC Amarillo was developed at the University of Saskatchewan, and has a strong agronomic performance, good fungal disease resistance, high grain yield, high seed protein concentration, and is widely grown in western Canada [48][49][50][51]. CDC Amarillo is also widely available for vDAO extraction.…”

Section: Discussionmentioning

confidence: 99%

Diamine Oxidase as a Therapeutic Enzyme: Study of Germination from Vegetal Sources and Investigation of the Presence of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) Using LC-MS/MS

Boulfekhar

Ohlund

Kumaresan

et al. 2023

IJMS

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Vegetal diamine oxidase (vDAO), an enzyme proposed to relieve symptoms of histaminosis, shows better reactivity with histamine and aliphatic diamines, as well as higher enzymatic activity than DAO of animal origin. The objective of this study was to evaluate the enzyme activity of vDAO from germinating grains from Lathyrus sativus (grass pea) and Pisum sativum (pea), and to verify the presence of a neurotoxin, β-N-Oxalyl-L-α,β-diaminopropionic acid (β-ODAP), in the crude extract obtained from their seedlings. A targeted liquid chromatography–multiple-reaction monitoring mass spectrometry method was developed and used to quantify β-ODAP in the analysed extracts. An optimized sample preparation procedure, involving protein precipitation with acetonitrile followed by mixed-anion exchange solid-phase extraction, allowed for high sensitivity and good peak shape for β-ODAP detection. The Lathyrus sativus extract exhibited the highest vDAO enzyme activity of the extracts, followed by the extract from pea cultivar Amarillo from the Crop Development Centre (CDC). The results have also shown that even though β-ODAP was present in the crude extract from L. sativus, its content was far below the toxicity threshold (300 mg of β-ODAP/kg body/day). CDC Amarillo showed 5000-fold less β-ODAP than the undialysed L. sativus extract. It was concluded that both species can be considered as convenient sources of vDAO for potential therapeutic use.

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Section: Discussionmentioning

confidence: 99%

Diamine Oxidase as a Therapeutic Enzyme: Study of Germination from Vegetal Sources and Investigation of the Presence of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) Using LC-MS/MS

Boulfekhar

Ohlund

Kumaresan

et al. 2023

IJMS

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“…Yellow pea seeds (CDC Inca) were provided by the Crop Development Centre, University of Saskatchewan, Canada (Warkentin et al, 2017). Pea plants were grown in 4″ pots in Sunshine No.…”

Section: Methodsmentioning

confidence: 99%

“…CRISPR/Cas9 genome editing has been successfully used to edit phytoene desaturase (Li et al, 2023) and lipoxygenase 2 (Bhowmik et al, 2023) in peas. In this work, we optimized CRISPR/Cas9 using induced hairy roots from an elite yellow pea cultivar, CDC Inca (Warkentin et al, 2017). The developed system was then leveraged to produce two mutant alleles in stable mutant lines with a 99.8% reduction of saponin concentration in pea seeds.…”

Section: Introductionmentioning

confidence: 99%

Creating saponin‐free yellow pea seeds by CRISPR/Cas9‐enabled mutagenesis on β‐amyrin synthase

Hodgins,

Salama,

Kumar

et al. 2024

Plant Direct

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Dry pea (Pisum sativum) seeds are valuable sources of plant protein, dietary fiber, and starch, but their uses in food products are restricted to some extent due to several off‐flavor compounds. Saponins are glycosylated triterpenoids and are a major source of bitter, astringent, and metallic off‐flavors in pea products. β‐amyrin synthase (BAS) is the entry point enzyme for saponin biosynthesis in pea and therefore is an ideal target for knock‐out using CRISPR/Cas9 genome editing to produce saponin deficient pea varieties. Here, in an elite yellow pea cultivar (CDC Inca), LC/MS analysis identified embryo tissue, not seed coat, as the main location of saponin storage in pea seeds. Differential expression analysis determined that PsBAS1 was preferentially expressed in embryo tissue relative to seed coat and was selected for CRISPR/Cas9 genome editing. The efficiency of CRISPR/Cas9 genome editing of PsBAS1 was systematically optimized in pea hairy roots. From these optimization procedures, the AtU6‐26 promoter was found to be superior to the CaMV35S promoter for gRNA expression, and the use of 37°C was determined to increase the efficiency of CRISPR/Cas9 genome editing. These promoter and culture conditions were then applied to stable transformations. As a result, a bi‐allelic mutation (deletion and inversion mutations) was generated in the PsBAS1 coding sequence in a T1 plant, and the segregated psbas1 plants from the T2 population showed a 99.8% reduction of saponins in their seeds. Interestingly, a small but statistically significant increase (~12%) in protein content with a slight decrease (~5%) in starch content was observed in the psbas1 mutants under phytotron growth conditions. This work demonstrated that flavor‐improved traits can be readily introduced in any pea cultivar of interest using CRISPR/Cas9. Further field trials and sensory tests for improved flavor are necessary to assess the practical implications of the saponin‐free pea seeds in food applications.

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“…The estimated commercial value of pea seeds, with high protein content based on the average retail price of pea protein isolate in the range of 25–30 USD/kg of isolate, has led to an increased focus on plant breeding programs that aim to develop pea cultivars with higher SPC. In the last decade, the pea breeding program at the Crop Development Centre (CDC) at the University of Saskatchewan has developed cultivars such as CDC Amarillo ( Warkentin et al., 2014a ), CDC Limerick ( Warkentin et al., 2014b ), and CDC Inca ( Warkentin et al., 2018 ) with improved SPC of up to 25%. It is well known in field peas that SPC is negatively correlated with grain yield (GY) ( Tar’an et al., 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs associated with seed protein concentration in two diverse recombinant inbred line populations of pea

Gali,

Jha,

Tar’an

et al. 2024

Front. Plant Sci.

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Improving the seed protein concentration (SPC) of pea (Pisum sativum L.) has turned into an important breeding objective because of the consumer demand for plant-based protein and demand from protein fractionation industries. To support the marker-assisted selection (MAS) of SPC towards accelerated breeding of improved cultivars, we have explored two diverse recombinant inbred line (RIL) populations to identify the quantitative trait loci (QTLs) associated with SPC. The two RIL populations, MP 1918 × P0540-91 (PR-30) and Ballet × Cameor (PR-31), were derived from crosses between moderate SPC × high SPC accessions. A total of 166 and 159 RILs of PR-30 and PR-31, respectively, were genotyped using an Axiom® 90K SNP array and 13.2K SNP arrays, respectively. The RILs were phenotyped in replicated trials in two and three locations of Saskatchewan, Canada in 2020 and 2021, respectively, for agronomic assessment and SPC. Using composite interval mapping, we identified three QTLs associated with SPC in PR-30 and five QTLs in PR-31, with the LOD value ranging from 3.0 to 11.0. A majority of these QTLs were unique to these populations compared to the previously known QTLs for SPC. The QTL SPC-Ps-5.1 overlapped with the earlier reported SPC associated QTL PC-QTL-3. Three QTLs, SPC-Ps-4.2, SPC-Ps-5.1, and SPC-Ps-7.2 with LOD scores of 7.2, 7.9, and 11.3, and which explained 14.5%, 11.6%, and 11.3% of the phenotypic variance, respectively, can be used for marker-assisted breeding to increase SPC in peas. Eight QTLs associated with the grain yield were identified with LOD scores ranging from 3.1 to 8.2. Two sets of QTLs, SPC-Ps-2.1 and GY-Ps-2.1, and SPC-Ps-5.1 and GY-Ps-5.3, shared the QTL/peak regions. Each set of QTLs contributed to either SPC or grain yield depending on which parent the QTL region is derived from, thus confirming that breeding for SPC should take into consideration the effects on grain yield.

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CDC Inca yellow field pea

Cited by 4 publications

References 2 publications

Diamine Oxidase as a Therapeutic Enzyme: Study of Germination from Vegetal Sources and Investigation of the Presence of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) Using LC-MS/MS

Diamine Oxidase as a Therapeutic Enzyme: Study of Germination from Vegetal Sources and Investigation of the Presence of β-N-Oxalyl-L-α,β-diaminopropionic Acid (β-ODAP) Using LC-MS/MS

Creating saponin‐free yellow pea seeds by CRISPR/Cas9‐enabled mutagenesis on β‐amyrin synthase

Identification of QTLs associated with seed protein concentration in two diverse recombinant inbred line populations of pea

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