CDEMI: Characterizing differences in microbial composition and function in microbiome data

Wang, Lidan; Xiao, Liang; Chen, Hao; CAO, Lijie; Liu, Lan; Zhu, Feng; Ding, Yubin; Tang, Jing; XIE, Youlong

doi:10.1016/j.csbj.2023.03.044

Cited by 1 publication

(1 citation statement)

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“…Under natural conditions, microorganisms perform their functions as communities; hence, research is being increasingly directed toward the investigation of the functions of microbial communities and the construction of microbiomes rather than the functions of single microorganisms. 15 In a previous study, the characterization of microbial communities with lignocellulose-degrading abilities revealed that different microorganisms secrete and express complex enzyme systems related to lignocellulose degradation via synergistic interactions. 16 The widespread use of metagenomics provides a new approach for screening specific functional microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Construction of a Synthetic Microbial Community for Enzymatic Pretreatment of Wheat Straw for Biogas Production via Anaerobic Digestion

Chen,

Cai,

Wang

et al. 2024

Environ. Sci. Technol.

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Biological pretreatment is a viable method for enhancing biogas production from straw crops, with the improvement in lignocellulose degradation efficiency being a crucial factor in this process. Herein, a metagenomic approach was used to screen core microorganisms (Bacillus subtilis, Acinetobacter johnsonii, Trichoderma viride, and Aspergillus niger) possessing lignocellulose-degrading abilities among samples from three environments: pile retting wheat straw (WS), WS returned to soil, and forest soil. Subsequently, synthetic microbial communities were constructed for fermentation–enzyme production. The crude enzyme solution obtained was used to pretreat WS and was compared with two commercial enzymes. The synthetic microbial community enzyme-producing pretreatment (SMCEP) yielded the highest enzymatic digestion efficacy for WS, yielding cellulose, hemicellulose, and lignin degradation rates of 39.85, 36.99, and 19.21%, respectively. Furthermore, pretreatment of WS with an enzyme solution, followed by anaerobic digestion achieved satisfactory results. SMCEP displayed the highest cumulative biogas production at 801.16 mL/g TS, which was 38.79% higher than that observed for WS, 22.15% higher than that of solid-state commercial enzyme pretreatment and 25.41% higher than that of liquid commercial enzyme pretreatment. These results indicate that enzyme-pretreated WS can significantly enhance biogas production. This study represents a solution to the environmental burden and energy use of crop residues.

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Section: Introductionmentioning

confidence: 99%