cDNA clones completing the nucleotide and derived amino acid sequence of the alpha 1 chain of basement membrane (type IV) collagen from mouse

Wood, Linda; Theriault, Nicole Y.; Vögeli, Gabriel

doi:10.1016/0014-5793(88)81402-9

Cited by 54 publications

(9 citation statements)

References 14 publications

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“…[ ␣ -32 P]CTP (3,000 Ci/mmol) was from DuPont-NEN (Boston, MA). The cDNA probes used for Northern blot analysis were as follows: 1.3 kb Pst1-BamH1 fragment of p ␣ 1R1 for rat ␣ 1(I) collagen (23), 2.2 kb PvuII fragment of pRGR5 for rat pro-␣ 1(III) collagen (24), 0.62 kb PstI-PvuII fragment of pCV-1-C87 for mouse ␣ 1(IV) collagen (25), 0.27 kb HindIII-EcoRI fragment of p-SR270 for rat fibronectin (26), 1.3 kb EcoRI fragment of HL-40 for human laminin, B1 polypeptide (27), 2.1 kb EcoRI fragment of human matrix metalloproteinase 2 (MMP-2) (28), 0.6 kb EcoRI fragment of human tissue inhibitor of metalloproteinase 2 (TIMP-2), 3.9 kb the XbaI fragment of pTZ for human TIMP-1 (29), and 2.0 kb EcoRI fragment of hTGF ␤ -2 for human TGF-␤ 1 (30). The plasmids that contain the untranslated 5 Ј regions of the mouse ␣ 1(I) collagen gene linked to chloramphenicol acetyltransferase gene, pG 70, pG 100, and pG 60 were the generous gift of Dr. B. de Crombrugghe (31).…”

Section: Methodsmentioning

confidence: 99%

Regulation of extracellular matrix by mechanical stress in rat glomerular mesangial cells.

Yasuda¹,

Kondo²,

Homma³

et al. 1996

J. Clin. Invest.

140

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Increases in intraglomerular pressure are known to predispose to the development of glomerular sclerosis, which is characterized by accumulation of extracellular matrix within the glomerulus. Glomerular mesangial cells are exposed to pulsatile capillary pressures and are a potential target for mechanical stress. In the present studies, we subjected cultured rat mesangial cells to continuous cycles of stretching and relaxation (stretch/relaxation) and examined alterations in extracellular matrix gene expression.After 48

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Section: Methodsmentioning

confidence: 99%

Regulation of extracellular matrix by mechanical stress in rat glomerular mesangial cells.

Yasuda¹,

Kondo²,

Homma³

et al. 1996

J. Clin. Invest.

140

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“…A 3.4-kb EcoR-I fragment of a mouse «1(1 V) collagen cDNA from plasmid pBS was used to detect the 5.9-and 6.8-kb CIV transcript (14). This probe was purchased from ATCC.…”

Section: Extraction and Quantitation O F Mrnamentioning

confidence: 99%

Effects of Low Density Lipoprotein on Type IV Collagen Production by Cultured Rat Mesangial Cells

Kim

Kang

Cho

et al. 1994

Nephron

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Hyperlipidemia, especially hypercholesterolemia, may contribute to glomerulosclerosis as it does to atherosclerosis. Low density lipoprotein (LDL) stimulates the production of extracellular matrix by mesangial cells in culture as well as the proliferation of mesangial cells. This study was carried out to examine the effects of LDL on the type IV collagen (CIV) production by cultured rat mesangial cells (CRMC). Subconfluent CRMC monolayers which were grown in RPMI with 20% lipid-free fetal calf serum for 48 h were challenged with LDL (0, 50,100,150 and 200 μg/ml) for another 48 h. LDL was prepared from normal human plasma. Mesangial cell proliferation was examined by [³H]-thymidine uptake. Production of CIV was evaluated as the expression of CIV on the cell surface by flow-cytometric analysis. The collagen synthesis was measured by the [³H]-proline uptake. Total RNA was extracted from CRMC at 6 and 24 h of incubation with 150 μg/ml LDL, and Northern blotting and hybridization was performed with cDNAs for α_rCIV, for 72-kD collagenase and for tissue inhibitor of metalloproteinase (TIMP)-2. The amount of total mRNA was corrected with β-actin mRNA. Mesangial cell proliferation increased in all concentrations studied and had a peak value of 221% with 150 μg/ml of LDL. Expression of CIV increased by 30-60% in 100-200 μg/ml of LDL. Collagen synthesis also increased by 50-70% in 150-200 μg/ml of LDL. The mRNA ratio (procollagen α₁(IV)/β-actin) increased to 133% at 24 h. The mRNA ratio (TIMP-2/β-actin) increased to 137% at 24 h. mRNA ratios at 6 h showed no change. LDL had little effect on collagenase mRNA expression. These results show that LDL stimulates, in addition to stimulated mesangial cell proliferation, collagen production through a combination of increased collagen synthesis and possibly decreased collagen degradation. Hyperlipidemia may contribute to the pathogenesis of glomerulosclerosis through the direct effect of LDL on mesangial cells.

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“…Acknowledgments-We are grateful to Dr. Tor Ny for providing the rat tPA cDNA clone (35) and to Dr. Linda S. Wood for providing the mouse ␣-1 type IV collagen-cDNA (pCIV-1-PE16) clone (36).…”

mentioning

confidence: 99%

Interference with DNA Methyltransferase Activity and Genome Methylation during F9 Teratocarcinoma Stem Cell Differentiation Induced by Polyamine Depletion

Frostesjö

Holm

Grahn

et al. 1997

Journal of Biological Chemistry

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When ornithine decarboxylase, the initial and highly regulated enzyme in polyamine biosynthesis, is irreversibly inactivated by ␣-difluoromethylornithine, F9 teratocarcinoma stem cells are depleted of putrescine and spermidine and as a result differentiate into a cell type which phenotypically resembles the parietal endoderm cells of the early mouse embryo. Simultaneously the level of decarboxylated S-adenosylmethionine (dcAdoMet), the aminopropyl group donor in spermidine and spermine synthesis, increases dramatically, as the aminopropyl group acceptor molecules (putrescine and spermidine) become limiting. When this excessive accumulation of dcAdoMet is prevented by specific inhibition of the AdoMet decarboxylase activity, the differentiative effect is counteracted, despite the fact that the extent of polyamine depletion remains almost identical. Therefore, it may be concluded that dcAdoMet plays an important role in the induction of differentiation. Moreover, this key metabolite acts as a competitive inhibitor of DNA methyltransferase and is therefore capable of interfering with the maintenance methylation of newly replicated DNA. During the course of F9 cell differentiation, the highly methylated genome is gradually demethylated, and its pattern of gene expression is changed. Our present findings, that the DNA remains highly methylated and that the differentiative process is counteracted when the build-up of dcAdoMet is prevented, provide strong evidence for a causative relation between the level of dcAdoMet and the state of DNA methylation as well as cell differentiation.Cells require optimal levels of the polyamines, putrescine, spermidine, and spermine, for their growth and differentiation and are therefore equipped with many intricate mechanisms for the control of these levels (1, 2). The polyamine biosynthetic pathway consists of two highly regulated enzymes, ornithine decarboxylase (ODC) 1 and S-adenosylmethionine decarboxylase (AdoMetDC), and two constitutively expressed enzymes, spermidine synthase and spermine synthase (Fig. 1). Any cell that becomes polyamine-deficient is severely limited in its ability to grow and proliferate. There is also evidence suggesting that polyamines play a role in apoptotic cell death (3, 4). More specifically, the polyamines have been shown to regulate ion channel gating (5-7) and polyamine derivatives are constituents of venoms produced by many invertebrates (e.g. funnel-web spiders), where they act as potent neurotoxins (8). Polyamines also regulate programmed translational frameshifting, which is essential for the expression of ODC antizyme, a protein involved in the regulation of polyamine levels both in yeast (9) and in mammalian (10) cells.Of particular interest, with regard to growth regulation, is the finding that when ODC genes are transfected into cells and overexpressed, the cells go through malignant transformation (11). The fact that inhibition of the ODC activity counteracts malignant transformation suggests that the ODC gene is a proto-oncogene. In this co...

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cDNA clones completing the nucleotide and derived amino acid sequence of the alpha 1 chain of basement membrane (type IV) collagen from mouse

Cited by 54 publications

References 14 publications

Regulation of extracellular matrix by mechanical stress in rat glomerular mesangial cells.

Regulation of extracellular matrix by mechanical stress in rat glomerular mesangial cells.

Effects of Low Density Lipoprotein on Type IV Collagen Production by Cultured Rat Mesangial Cells

Interference with DNA Methyltransferase Activity and Genome Methylation during F9 Teratocarcinoma Stem Cell Differentiation Induced by Polyamine Depletion

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