cDNA Cloning and Characterization of the Human Interleukin 13 Receptor α Chain

Abstract

Interleukin 13 (IL-13)1 is a T-cell-derived cytokine that exhibits a broad range of activities in the regulation of inflammatory and immune responses. Many of the actions of IL-13 are also exhibited by another T-cell-derived cytokine, IL-4, with which it shares approximately 30% amino acid sequence identity. These shared activities include down-regulation of inflammatory cytokines (1), induction of expression of the IL-1 receptor antagonist (2), induction of surface expression of class II major histocompatibility complex (3), induction of CD23 and IgE expression on B cells (4), costimulation with anti

Adenoviral vector–mediated gene transfer of IL‐13Rα2 chain followed by IL‐13 cytotoxin treatment offers potent targeted therapy for cytotoxin‐resistant cancers

“…Previous studies have demonstrated that the attachment of radiolabeled IL-13 to the functional heterodimer receptor can be competed by IL-4 as well as IL-13, [30][31][32]49 whereas its attachment to IL-13R 2 is competed by IL-13 but not IL-4. 2,9,10,15,33,36 To confirm that the cytotoxicity of IL-13 cytotoxin was specifically mediated through IL-13R 2 chain delivered by Ad-IL-13R 2 vector, A549 cells were infected with Ad-IL-13R 2 at MOI 10 followed by treatment with IL-13 cytotoxin (10 ng/ml) in the presence of a 50-fold dose of human IL-13 or IL-4.…”

“…24,25 It is critical for tumor immunosurveillance 26,27 and modulates apoptosis or tumor cell growth. 28,29 Two different components of the IL-13 receptor have been identified, which are known as IL-13R 1 chain [30][31][32] and IL-13R 2 chain. 33,34 IL-13R 1 chain alone binds IL-13 with low affinity.…”

“…33,34 IL-13R 1 chain alone binds IL-13 with low affinity. Therefore, IL-13R 1 chain requires the recruitment of IL-4R chain [30][31][32]35 to form a high-affinity heterodimer receptor complex, which mediates the IL-13 signaltransduction pathway. IL-13R 2 chain by itself can bind IL-13 with higher affinity, but it does not mediate IL-13 signaling 35,36 because it has a very short cytoplasmic domain that lacks the conserved signaling motifs.…”

The biology of Stat4 and Stat6

“…The overlapping functional and biochemical activities of IL-4 and IL-13 can be explained by the fact that their receptors share the IL-4Ra chain (Lin et al, 1995;Zurawski et al, 1995). Unlike IL-4, the IL-4Ra chain is unable to recognize IL-13 by itself, but when combined with the IL-13Ra chain the complex binds IL-13 with a high a nity (Aman et al, 1996;Hilton et al, 1996).…”

Allergen-dependent solubilization of IL-13 receptor α2 reveals a novel mechanism to regulate allergy

“…[7][8][9][10][11][12] IL-13 has 2 cognate receptors, IL-13 receptor α1 (IL-13Rα1) and IL-13 receptor α2 (IL-13α2). [13][14][15][16][17][18] IL-13Rα1 binds IL-13 with low affinity by itself, but when paired with IL-4 receptor α, it binds IL-13 with high affinity, forming a signaling IL-13 receptor. 16 IL-13Rα2 binds IL-13 with high affinity and acts as a decoy receptor, as shown by IL-13Rα2 knockout mice.…”