cDNA cloning and eukaryotic expression of feline CD9

Willett, Brian J.; Neil, James C.

doi:10.1016/0161-5890(95)00008-3

Cited by 16 publications

(11 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The finding that both human and feline CD9 effect very similar increases in motility involving ␤1 integrins confirms that CD9 modulates migratory responses to extracellular matrix proteins and is consonant with the 95.1% homology in amino acid sequence between the two proteins (24). However, the effectiveness of anti-VLA-6 mAb in inhibiting the motile response of Raji/CD9f, but not of Raji/CD9h, on fibronectin substrates suggests a regulatory role for those regions of CD9 which are differentially expressed including amino acids 169 -180 of the second external loop and a potential N-linked glycosylation site within the first external loop (24). Our finding that an anti-VLA-4 mAb inhibited migration of the transfectants on laminin to which VLA-4 is not known to bind was unexpected.…”

Section: Cd9 Enhanced Motility and Tyrosine Phosphorylationsupporting

confidence: 56%

“…To investigate the possibility that CD9 plays a role in integrin-dependent motility, we transfected cDNA encoding human and feline CD9 into the immature B cell Raji which lacks CD9. Raji expresses the ␤1 integrin VLA-4, a fibronectin receptor found in highly motile cells (22)(23)(24)(25)(26) implicated in invasiveness, and metastasis (22,23), as well as the laminin receptor VLA-6. In our experiments, Raji cells penetrated laminin or fibronectin-coated filters poorly, suggesting they might lack an accessory molecule required for migration on integrin-dependent substrates.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Ectopic Expression of Human and Feline CD9 in a Human B Cell Line Confers β1 Integrin-dependent Motility on Fibronectin and Laminin Substrates and Enhanced Tyrosine Phosphorylation

Shaw

Domanska

Mak

et al. 1995

Journal of Biological Chemistry

124

View full text Add to dashboard Cite

Few molecules have been shown to confer cell motility. Although the motility-arresting properties of anti-CD9 monoclonal antibody (mAb) suggest the transmembrane 4 superfamily (TM4SF) member CD9 can induce a motorgenic signal, gene transfection studies have failed to confirm this hypothesis. We report here that ectopic expression of human CD9 (CD9h) and feline CD9 (CD9f) in the CD9-negative, poorly motile, human B cell line Raji dramatically enhances migration across fibronectin-and laminin-coated polycarbonate filters. Migration of Raji/CD9h and Raji/CD9f on either substrate was inhibited by the anti-CD9 mAb 50H.19 and by the anti-␤1 integrin mAb AP-138. Migration of Raji/CD9h on laminin was potently inhibited by the anti-VLA-6 integrin mAb GoH3 and by the anti-VLA-4 integrin mAb 44H6, whereas migration of Raji/CD9h on fibronectin was inhibited only by mAb 44H6. Since CD9h-transfected Raji cells adhered to fibronectin as effectively as mock transfectants, expression of CD9 enhanced motility, but not adhesion. CD9-enhanced migration was inhibited by the protein tyrosine kinase inhibitor herbimycin A suggesting that tyrosine phosphorylation played a role in the generation of a motorgenic signal. Raji/CD9h transfectants adherent to fibronectin expressed 6-fold higher levels of phosphotyrosine than Raji. Raji/CD9f transfectants also phosphorylated proteins on tyrosine more effectively than Raji including a protein of 110 kDa which was phosphorylated on the motility-inducing substrates laminin and fibronectin, but not on bovine serum albumin. Our results support a role for CD9 in the amplification of a motorgenic signal in B cells involving ␤1 integrins and the activation of protein tyrosine kinases.

show abstract

Section: Cd9 Enhanced Motility and Tyrosine Phosphorylationsupporting

confidence: 56%

mentioning

confidence: 99%

Ectopic Expression of Human and Feline CD9 in a Human B Cell Line Confers β1 Integrin-dependent Motility on Fibronectin and Laminin Substrates and Enhanced Tyrosine Phosphorylation

Shaw

Domanska

Mak

et al. 1995

Journal of Biological Chemistry

124

View full text Add to dashboard Cite

show abstract

“…Primary antibodies were detected using fluorescein isothiocyanate conjugated F(abh) # fragment of sheep anti-mouse IgG whole molecule (Sigma). Cells were processed for flow cytometry as described previously (Willett & Neil, 1995). Analysis was performed on an EPICS Elite flow cytometer (Coulter Electronics).…”

Section: Introductionmentioning

confidence: 99%

“…encoding the feline homologues of CD9 and CD29 were generated from mRNA from the IL-2-dependent feline T cell line Mya-1, which is highly susceptible to infection with both primary and CrFK-adapted isolates of FIV. The cDNA cloning and eukaryotic expression of feline CD9 have been described (Willett & Neil, 1995). Poly(A) + RNA was prepared from the Mya-1 cell line using a FastTrack mRNA isolation kit (Invitrogen).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of feline immunodeficiency virus infection by CD9 antibody operates after virus entry and is independent of virus tropism.

Willett¹,

Hosie²,

Shaw³

et al. 1997

Journal of General Virology

Self Cite

View full text Add to dashboard Cite

A monoclonal antibody which blocks infection with feline immunodeficiency virus (FIV) was found previously to react with the cell surface molecule CD9, implicating CD9 in the process of virus entry. We report here that inhibition by anti-CD9 antibody does not operate at the level of virus entry but at a subsequent stage in the virus life-cycle. Moreover, inhibition of infection is independent of the passage history of the virus or the virus subtype. Inhibition of

show abstract

“…1992), mouse (Rubinstein et al. 1993), feline (Willett and Neil 1995), rat (Kaprielian et al. 1995), chicken (Kobayashi 2000), Atlantic salmon (Fujiki et al.…”

Section: Introductionmentioning

confidence: 99%

Molecular Cloning and Characterization of CD9 cDNA from Sheep and Cashmere Goat

Xing

et al. 2010

Reprod Domestic Animals

View full text Add to dashboard Cite

CD9 is a glycoprotein of the transmembrane 4 superfamily (TM4SF) and is involved in various cellular processes. Some CD9 cDNA have been cloned in mammals and certain fish genera in recent years, but goat and sheep counterparts of cattle, human and mouse have not been identified. To facilitate the studies, we cloned the cDNA encoding for CD9 of cashmere goat (Capra hircus) and sheep (Ovis aries), and expressed sheep CD9 in Escherichia coli cells. Structural analysis indicated for both goat and sheep that a 1123 bp cDNA spanned an open reading frame of 681 bp which predicted a protein of 226 amino acids with a typical TM4SF structure, including four highly conserved transmembrane domains, two extracellular domains and a CCG motif, which is a hallmark of the TM4SF. The predicted amino acid sequences were highly homologous to those of cattle, mouse and human CD9. Molecular phylogenetic analysis based on CD9 cDNA sequences indicated that goat and sheep CD9 were closely related to CD9 of cattle, which is in agreement with their morphological taxonomy.

show abstract

cDNA cloning and eukaryotic expression of feline CD9

Cited by 16 publications

References 23 publications

Ectopic Expression of Human and Feline CD9 in a Human B Cell Line Confers β1 Integrin-dependent Motility on Fibronectin and Laminin Substrates and Enhanced Tyrosine Phosphorylation

Ectopic Expression of Human and Feline CD9 in a Human B Cell Line Confers β1 Integrin-dependent Motility on Fibronectin and Laminin Substrates and Enhanced Tyrosine Phosphorylation

Inhibition of feline immunodeficiency virus infection by CD9 antibody operates after virus entry and is independent of virus tropism.

Molecular Cloning and Characterization of CD9 cDNA from Sheep and Cashmere Goat

Contact Info

Product

Resources

About