cDNA cloning and expression pattern of two enolase genes from the Chinese oak silkworm, <italic>Antheraea pernyi</italic>

Abstract

In this study, two enolase genes were isolated and characterized from the Chinese oak silkworm, Antheraea perny, which were designated as enolase I and II, respectively. The enolase I cDNA sequence was 1712 bp with an open reading frame (ORF) of 1302 bp encoding 433 amino acids. The enolase II cDNA sequence was 1549 bp with an ORF of 1296 bp encoding 431 amino acids. The amino acid sequences of the two genes share several conserved features/sites of enolase. Antheraea pernyi enolase I shows 93% -97% sequence identity to enolases of lepidopterans available to date, 75% -82% identity to enolases of other invertebrates, 60% -72% identity to enolases of other organisms including vertebrates, plants, and fungi. Antheraea pernyi enolase II shows 84% identity to Bombyx mori enolase II, but 60% identity to A. pernyi enolase I. In the phylogenetic tree, enolase II sequences from A. pernyi and B. mori were clearly separated from the majority of enolase sequences of higher organisms including A. pernyi and B. mori enolase I sequences. By sequence comparisons and phylogenetic analysis, we suggest that enolase II from A. pernyi and B. mori may be a new member of the enolase superfamily. Antheraea pernyi enolase I mRNA was found in all tested tissues whereas enolase II mRNA was expressed specifically in the spermaries and ovaries, suggesting that the product of enolase II gene may be related to reproduction. The transcript abundance of A. pernyi enolase I gene was significantly down-regulated after cold shock and significantly up-regulated after heat shock, suggesting that A. pernyi enolase I gene may be inducible by temperature stress.

Molecular Cloning, Expression Pattern, and Phylogenetic Analysis of a Selenophosphate synthetase Gene from Antheraea pernyi (Lepidoptera: Saturniidae)

“…The phylogenetic trees constructed in this study supported the monophyly of Lepidoptera, Diptera, Coleoptera, and Hymenoptera. These results are consistent with the traditional classiÞcation, and other molecular data, such as enolase gene (Liu et al 2010b), lysophospholipase gene (Liu et al 2010c), and will die slowly gene ).…”

“…In recent years, many housekeeping genes, such as ribosomal protein gene (Hou et al 2008), myosin light chain 2 gene (Yang et al 2008, Liu et al 2010d, enolase gene (Regier et al 2009, Liu et al 2010b, lysophospholipase gene (Liu et al 2010c), and will die slowly gene , have been reported to be a new molecular tool for phylogenetic inference of life organs. Our study showed that the phylogenetic analysis based on the Sps amino acid sequences clearly separated the known bacterial, fungal, invertebrate, and vertebrate Sps proteins.…”

Identification of functional enolase genes of the silkworm Bombyx mori from public databases with a combination of dry and wet bench processes

“…The Chinese oak moth ( Antheraea pernyi ) is a lepidopteran insect, and its enolaseI and enolaseII genes corresponded well with BmEno1 and BmEno2. A. pernyi enolaseII contains the same metal-binding and substrate-binding amino acid residues as BmEno2 and BmEnoC [17]. Our phylogenetic analysis showed that BmEno1 was close to the cluster containing insect enolases, such as those from D. melanogaster , T. castaneum and A. mellifera .…”

Fructose-bisphosphate aldolase and enolase from Echinococcus granulosus: Genes, expression patterns and protein interactions of two potential moonlighting proteins

“…In invertebrates, there are evidences for the presence of more than one enolase gene with differential regulation. For instance, two enolase genes have been reported for the silkworm Antheraea pernyi (Liu et al, 2010) and for Toxoplasma gondii (Dzierszinski et al, 2001). In S. japonicum, southern blot analysis suggested that this parasite also contains more than one enolase gene (Waine et al, 1993).…”