2004
DOI: 10.1074/jbc.m410098200
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cDNA Cloning and Functional Expression of a Ca2+-sensing Receptor with Truncated C-terminal Tail from the Mozambique Tilapia (Oreochromis mossambicus)

Abstract: The complete cDNA sequence of the tilapia extracellular Ca 2؉ -sensing receptor (CaR) was determined. The transcript length of tilapia CaR (tCaR) is 3.4 kbp and encodes a 940-amino acid, 7-transmembrane domain protein that is consistent in its structural features with known mammalian and piscine CaRs. The tCaR extracellular domain includes a characteristic hydrophobic segment, conserved cysteine residues that are implicated in receptor dimerization (Cys 129 and Cys 131 ) and in coupling to the transmembrane do… Show more

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Cited by 56 publications
(29 citation statements)
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“…In this study, we provided evidence that the treatment of cells with Ca 2C or Ca 2C /calcimimetics activated PLC-ERK and IP 3 -CaMK2 pathways. The data on PLC-ERK activation are consistent with reports on ectopic expression of tilapia or human CaSR in embryonic kidney cells (Loretz et al 2004, Davies et al 2006 or in bovine parathyroid cells respectively (Kifor et al 2001). Although the activation of CaSR-induced PLC-IP 3 signaling in fish tissues has not been reported previously, a recent study using isolated mouse juxtaglomerular cells described this post-receptor IP 3 pathway (Ortiz-Capisano et al 2013).…”
Section: Discussionsupporting
confidence: 79%
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“…In this study, we provided evidence that the treatment of cells with Ca 2C or Ca 2C /calcimimetics activated PLC-ERK and IP 3 -CaMK2 pathways. The data on PLC-ERK activation are consistent with reports on ectopic expression of tilapia or human CaSR in embryonic kidney cells (Loretz et al 2004, Davies et al 2006 or in bovine parathyroid cells respectively (Kifor et al 2001). Although the activation of CaSR-induced PLC-IP 3 signaling in fish tissues has not been reported previously, a recent study using isolated mouse juxtaglomerular cells described this post-receptor IP 3 pathway (Ortiz-Capisano et al 2013).…”
Section: Discussionsupporting
confidence: 79%
“…In the present study, our western blotting analysis data support the presence of Casr expression in gill tissues of Japanese eels. The molecular size of the eel Casr protein is similar to the expected molecular mass of non-glycosylated Casr core protein in tilapia (Loretz et al 2004). Moreover the treatment of isolated gill cells with Ca 2C or Ca 2C / calcimimetics activated the conserved CaSR-mediated pathways to increase PLC activity and ERK-and IP 3 / CaMK2-signaling.…”
Section: Discussionmentioning
confidence: 67%
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“…Not surprisingly, posttranslational modifications such as phosphorylation represent a prominent mechanism for osmosensory signal transduction. For instance, phospholipase C (PLC) and mitogen-activated protein kinase (MAPK) signaling pathways are involved in osmosensing in tilapia (Loretz et al, 2004). MAPK pathways also play a role in osmosensing in killifish and turbot (Kültz and Avila, 2001;Marshall et al, 2005).…”
Section: Evolution Of High Salinity Tolerance In Euryhaline Fishesmentioning
confidence: 99%
“…are euryhaline teleosts that inhabit environments ranging from freshwater to seawater of high salinity and can withstand temperatures as high as 42°C (31,32). Due to their tolerance of relatively extreme environments, tilapias have gained important roles as model organisms in the laboratory (31,33,34) and as a prominent fish in aquaculture (32,35). In this study, we have chosen the Mozambique tilapia (Oreochromis mossambicus) as a model system because the cardiac NCX from this species has likely adapted to function at temperatures similar to those in the mammalian heart, but lethal to trout.…”
mentioning
confidence: 99%