cDNA cloning and in situ hybridization of Δ11-desaturase, a key enzyme of pheromone biosynthesis in Ostrinia scapulalis (Lepidoptera: Crambidae)

Fukuzawa, Mai; Fu, Xin; Tatsuki, Sadahiro; Ishikawa, Yukio

doi:10.1016/j.jinsphys.2005.11.004

Cited by 13 publications

(15 citation statements)

References 22 publications

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“…In situ hybridizations using the Δ11-desaturase as probe were performed in the sister species O. scapulalis, and revealed that the enzyme producing the fatty acyl precursors is expressed only in the dorsal part of the terminal abdominal segments. The molecular observations thus corroborate the biochemical results (Fukuzawa et al, 2006). Similar results are to be expected for O. nubilalis.…”

Section: Location Of Production Sitesupporting

confidence: 90%

Journey in the Ostrinia World: From Pest to Model in Chemical Ecology

Lassance

2010

J Chem Ecol

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The European corn borer Ostrinia nubilalis (ECB; Lepidoptera: Crambidae) is a widely recognized pest of agricultural significance over much of the northern hemisphere. Because of the potential value of pheromone-based control, there has been considerable effort devoted to elucidation of the ECB chemical ecology. The species is polymorphic regarding its female-produced pheromone. Partly because of this feature, over the years the ECB has become a model to study pheromone evolution. This review should assist in identifying new areas of pheromone research by providing an overview of the literature produced on this subject for the ECB since the late 1960's.

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Section: Location Of Production Sitesupporting

confidence: 90%

Journey in the Ostrinia World: From Pest to Model in Chemical Ecology

Lassance

2010

J Chem Ecol

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“…5. Possible biosynthetic pathways and enzymes (genes) involved in the production of pheromone gland components in O. furnacalis, O. scapulalis, and their hybrid females inferred from the findings of the present study and information available on pheromone biosynthesis in a congener, O. nubilalis (Fukuzawa et al, 2006;Roelofs et al, 2002;Tillman et al, 1999;Zhao and Wang, 1990;Zhao et al, 1995;Zhu et al, 1996). The symbol resembling a rotated ''T'' indicates inhibition.…”

Section: Unexpected Problemsmentioning

confidence: 79%

“…Cultures of O. scapulalis that are monomorphic in terms of the production of E-type pheromone (>99% E11-14:OAc) were established from females collected at Matsudo (35.8 N,139.9 E), as described by Fukuzawa et al (2006). Cultures of O. furnacalis were established from females collected at Akigawa (35.4 N,139.2 E) in May 2005.…”

Section: Insectsmentioning

confidence: 99%

Alternative suppression of transcription from two desaturase genes is the key for species-specific sex pheromone biosynthesis in two Ostrinia moths

Sakai

Fukuzawa

Nakano

et al. 2009

Insect Biochemistry and Molecular Biology

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“…After Proteinase K treatment, SfiI digestion, and purification with a chroma spin column (Clontech), the normalized cDNA was ethanol precipitated and dissolved in PCR water (Advantage 2 PCR kit, Clontech) to a concentration of 100 ng/ml. The normalized cDNA was checked for the presence of the D11-desaturase gene by PCR using desL1/desR1 primers (Fukuzawa et al, 2006).…”

Section: Cdna Normalizationmentioning

confidence: 99%