cDNA cloning and molecular analysis of two self-incompatibility alleles from apple

Broothaerts, W.; Janssens, Gustaaf; Proost, Paul; Broekaert, Willem F.

doi:10.1007/bf00019317

Cited by 187 publications

(83 citation statements)

References 42 publications

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“…The molecular identification of the pistil components of SI has revealed three distinct mechanisms in the sporophytic SI system of Brassica and in the gametophytic SI systems of the Solanaceae/Rosaceae/ Scrophulariaceae and of Papaver (De Nettancourt, 2001). In the Solanaceae, the Rosaceae, and the Scrophulariaceae, the stylar part codes for a ribonuclease (S-RNase) (McClure et al, 1989;Broothaerts et al, 1995;Boš ković and Tobutt, 1996;Xue et al, 1996). Ribonuclease activity of S-RNases is needed to inhibit the growth of pollen tubes carrying an S allele that matches an S allele present in the style (Huang et al, 1994;Royo et al, 1994), supporting a model for this type of gametophytic SI in which pollen RNA is degraded in an incompatible interaction.…”

Section: Introductionmentioning

confidence: 90%

Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype–Specific F-Box Gene

et al. 2004

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Recently, an S haplotype-specific F-box (SFB) gene has been proposed as a candidate for the pollen-S specificity gene of RNase-mediated gametophytic self-incompatibility in Prunus (Rosaceae). We have examined two pollen-part mutant haplotypes of sweet cherry (Prunus avium). Both were found to retain the S-RNase, which determines stylar specificity, but one (S 3 9 in JI 2434) has a deletion including the haplotype-specific SFB gene, and the other (S 4 9 in JI 2420) has a frame-shift mutation of the haplotype-specific SFB gene, causing amino acid substitutions and premature termination of the protein.The loss or significant alteration of this highly polymorphic gene and the concomitant loss of pollen self-incompatibility function provides compelling evidence that the SFB gene encodes the pollen specificity component of self-incompatibility in Prunus. These loss-of-function mutations are inconsistent with SFB being the inactivator of non-self S-RNases and indicate the presence of a general inactivation mechanism, with SFB conferring specificity by protecting self S-RNases from inactivation.

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Section: Introductionmentioning

confidence: 90%

Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype–Specific F-Box Gene

et al. 2004

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“…At least one intron is expected, since the S-alleles of SRNases of Solanaceae and Rosaceae usually have a single intron (in the HVa region, from 87 bp to 120 bp, and 138 bp to 1100 bp, respectively, in sequences from these two families; Saba-El- Leil et al, 1994;Matton et al, 1995;Broothaerts et al, 1995). Prunus (Rosaceae) S-RNases have at least two introns (in P. avium, one 5′ to the C1 region, and one in HVa; Tao et al, 1999), and a sequence from P. dulcis (GenBank accession number AF157008) is similar (Figure 1).…”

Section: Analysis Of a Graniticum Crossesmentioning

confidence: 99%

“…The two HV regions are also found in S-RNases of Solanaceae, but only HVa is recognised in the S-RNases so far studied in the Rosaceae (Norioka et al, 1996;Sassa et al, 1996;Ushijima et al, 1998). The HV regions are the most hydrophilic regions of the S-proteins (Kao and McCubbin, 1996;Broothaerts et al, 1995;Xue et al, 1996), and may be on the external surface of the protein. Given their high degree of variability, they are candidates for the regions determining S-allele specificity.…”

Section: Introductionmentioning

confidence: 99%

Molecular variation at the self-incompatibility locus in natural populations of the genera Antirrhinum and Misopates

Vieira

Charlesworth

2002

Heredity

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The self-incompatibility system of flowering plants is a classic example of extreme allelic polymorphism maintained by frequency-dependent selection. We used primers designed from three published Antirrhinum hispanicum Sallele sequences in PCR reactions with genomic DNA of plants sampled from natural populations of Antirrhinum and Misopates species. Not surprisingly, given the polymorphism of S-alleles, only a minority of individuals yielded PCR products of the expected size. These yielded 35 genomic sequences, of nine different sequence types of which eight are highly similar to the A. hispanicum S-allele sequences, and one to a very similar unpublished Antirrhinum S-like

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“…Esse sistema apresenta três tipos de interação de compatibilidade: totalmente incompatível, quando ambos os alelos são comuns; parcialmente compatível em que apenas um alelo é diferente; e totalmente compatível, quando os quatro alelos são diferentes e a fecundação ocorre plenamente (KAUFMANN et al, 1992;NEWBI-GIN et al, 1993;LEWIS, 1994;SAKURAI, 2000;BRUCKNER,1994), Métodos que permitam avaliar antecipadamente a compatibilidade genética de alelos-S entre cultivares são de grande interesse, na medida em que permitem selecionar precocemente cultivares totalmente compatíveis, do ponto de vista genético-reprodutivo (MOTA;OLIVEIRA, 2005). Muito embora não permita substituir por completo os ensaios de polinização controlada a campo, a avaliação molecular dos alelos-S torna o processo de seleção de uma polinizadora mais eficiente (BROOTHAERTS et al, 1995;ISHIMIZU et al, 1999;YAEGAKI et al, 2001).…”

Section: Introductionunclassified

Caracterização molecular dos alelos-S de incompatibilidade gametofítica em Prunus salicina Lindl.

et al. 2010

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RESUMO-Pomares comerciais de ameixeira-japonesa devem conter pelo menos duas cultivares para obter boa fertilização, devido à presença do sistema de incompatibilidade gametofítica, que inibe a autofecundação da grande maioria das cultivares. No presente trabalho, buscou-se identificar e caracterizar molecularmente os alelos-S de 11 cultivares de ameixeira-japonesa (Prunus salicina Lindl.) e verificar a compatibilidade entre os genótipos avaliados. As cultivares Santa Rosa, Santa Rita, Reubennel, Pluma 7, América, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone São Paulo), Gulfblaze (Clone Guaiba) e Harry Pickstone foram analisadas por meio de Reação em Cadeia da Polimerase (PCR) com três pares de iniciadores específicos para alelos-S. As condições da PCR utilizadas, bem como as combinações de iniciadores, permitiram a identificação de alelos-S nas cultivares de P. salicina estudadas, bem como a indicação dos polinizadores mais compatíveis com algumas das principais cultivares utilizadas na produção de frutas. O sequenciamento de alguns dos alelos-S amplificados revelou elevada similaridade com sequências de nucleotídeos já identificados em outros trabalhos com Prunus spp.. Entretanto, a obtenção de sequências completas de maior número de alelos-S faz-se necessária para o estabelecimento de uma relação de identidade precisa entre os mesmos. Termos para Indexação: Ameixeira-japonesa, autoincompatibilidade gametofítica, PCR, alelos-S. MOLECULAR IDENTIFICATION OF S-ALLELES OF GAMETOPHYTIC INCOMPATIBILITY IN Prunus salicina Lindl.ABSTRACT -Commercial plum orchards must contain at least two cultivars, in order to ensure good fruit set and thus high economic yields, because its carries the gametophytic incompatibility system, that inhibits the self-pollination in a great number of cultivars. The aim of this work was to identify by PCR (Polymerase Chain Reaction) the S-alleles related to gametophytic incompatibility in eleven Prunus salicina (Lindl.) cultivars. The Santa Rosa, Santa Rita, Reubennel, Pluma 7, America, Rosa Mineira, Amarelinha, The First, Gulfblaze (Clone São Paulo), Gulfblaze (Clone Guaíba) e Harry Pickstone cultivars were analysed using three pairs of specific primers for amplifying the S-alleles. The PCR conditions and primers set allowed a characterization of S-alleles in the japonese plum and the identification of the most compatible pollinators to some commercial cultivars. Sequencing analysis of some amplified S-alleles showed high similarity to nucleotides sequences already identified in other studies with Prunus spp.. However, complete sequences of a large number of S-alleles it is necessary to get a more precisely identity of relationship among this S-alleles.

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cDNA cloning and molecular analysis of two self-incompatibility alleles from apple

Cited by 187 publications

References 42 publications

Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype–Specific F-Box Gene

Loss of Pollen-S Function in Two Self-Compatible Selections of Prunus avium Is Associated with Deletion/Mutation of an S Haplotype–Specific F-Box Gene

Molecular variation at the self-incompatibility locus in natural populations of the genera Antirrhinum and Misopates

Caracterização molecular dos alelos-S de incompatibilidade gametofítica em Prunus salicina Lindl.

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