cDNA Cloning, Characterization, and Functional Expression of Four New Monoterpene Synthase Members of the Tpsd Gene Family from Grand Fir (Abies grandis)

Bohlmann, Jörg; Phillips, Michael A.; Ramachandiran, Vasanthi; Katoh, Sadanobu; Croteau, Rodney

doi:10.1006/abbi.1999.1332

Cited by 128 publications

(80 citation statements)

References 29 publications

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“…Since deletion of the plastid targeting signal sequence has been reported to improve protein expression without altering enzyme activity or product specificity, this sequence was deleted to produce a pseudo-mature form of the protein. 17) We found that BcLS mainly generated linalool (86.5%), along with smaller amounts of myrcene (9.5%) and limonene (4.0%), with GPP as substrate ( Figs. 1 and 2).…”

Section: Characterization and Reaction Mechanism Of Bclsmentioning

confidence: 73%

Molecular Cloning and Characterization of a Linalool Synthase from Lemon Myrtle

Sugiura

Ito

Saito

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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Section: Characterization and Reaction Mechanism Of Bclsmentioning

confidence: 73%

Molecular Cloning and Characterization of a Linalool Synthase from Lemon Myrtle

Sugiura

Ito

Saito

et al. 2011

Bioscience, Biotechnology, and Biochemistry

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“…Despite extensive efforts of TPS gene discovery in species of spruce (14,17) and in grand fir (52), there is no known gene in any plant species that is closely related (Ͼ70% protein identity) to AbCAS. It is therefore possible that a CAS-type bifunctional class I/II diTPS is unique to balsam fir, which is the first conifer species for which three types of functionally distinct diTPSs are now known.…”

Section: Discussionmentioning

confidence: 99%

Bifunctional cis-Abienol Synthase from Abies balsamea Discovered by Transcriptome Sequencing and Its Implications for Diterpenoid Fragrance Production

Zerbe

Chiang

Yuen

et al. 2012

Journal of Biological Chemistry

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Background: Balsam fir produces cis-abienol, a natural product of value to the fragrance industry. Results: We describe the genomics-based discovery of balsam fir cis-abienol synthase. Conclusion: cis-Abienol synthase is a bifunctional diterpene synthase that produces a bicyclic diterpenol in the class II active site. Significance: cis-Abienol synthase is a new enzyme for metabolic engineering of plants or microorganisms to produce high value fragrance compounds.

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“…Unlike the often functionalized monoterpenes in the Lamiaceae, such as ())-menthol in peppermint (see above), most of the monoterpenes that accumulate in conifer oleoresin are the direct products of TPS and are not typically modified by other enzymes. The many conifer monoterpenes are formed from GDP by families of single-and multiple-product monoterpene synthases (Bohlmann et al, 1997(Bohlmann et al, , 1999Phillips et al, 2003). Biosynthesis of conifer diterpene resin acids involves two major steps (Keeling and Bohlmann, 2006b) after formation of GGDP: (i) conversion of GGDP to various, mostly tricyclic, diterpene olefin structures, catalysed by diterpene synthases, and (ii) three-step oxidation of the diterpene olefins at C18 to the corresponding diterpene resin acids (Figure 6).…”

Section: Conifer Oleoresin: Trapping Pests and Tapping Trees For Biommentioning

confidence: 99%

Terpenoid biomaterials

Bohlmann¹,

2008

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SummaryTerpenoids (isoprenoids) encompass more than 40 000 structures and form the largest class of all known plant metabolites. Some terpenoids have well-characterized physiological functions that are common to most plant species. In addition, many of the structurally diverse plant terpenoids may function in taxonomically more discrete, specialized interactions with other organisms. Historically, specialized terpenoids, together with alkaloids and many of the phenolics, have been referred to as secondary metabolites. More recently, these compounds have become widely recognized, conceptually and/or empirically, for their essential ecological functions in plant biology. Owing to their diverse biological activities and their diverse physical and chemical properties, terpenoid plant chemicals have been exploited by humans as traditional biomaterials in the form of complex mixtures or in the form of more or less pure compounds since ancient times. Plant terpenoids are widely used as industrially relevant chemicals, including many pharmaceuticals, flavours, fragrances, pesticides and disinfectants, and as large-volume feedstocks for chemical industries. Recently, there has been a renaissance of awareness of plant terpenoids as a valuable biological resource for societies that will have to become less reliant on petrochemicals. Harnessing the powers of plant and microbial systems for production of economically valuable plant terpenoids requires interdisciplinary and often expensive research into their chemistry, biosynthesis and genomics, as well as metabolic and biochemical engineering. This paper provides an overview of the formation of hemi-, mono-, sesqui-and diterpenoids in plants, and highlights some well-established examples for these classes of terpenoids in the context of biomaterials and biofuels.

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cDNA Cloning, Characterization, and Functional Expression of Four New Monoterpene Synthase Members of the Tpsd Gene Family from Grand Fir (Abies grandis)

Cited by 128 publications

References 29 publications

Molecular Cloning and Characterization of a Linalool Synthase from Lemon Myrtle

Molecular Cloning and Characterization of a Linalool Synthase from Lemon Myrtle

Bifunctional cis-Abienol Synthase from Abies balsamea Discovered by Transcriptome Sequencing and Its Implications for Diterpenoid Fragrance Production

Terpenoid biomaterials

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