cDNA Cloning of a Chick Homologue of Human ATPase Complex Subunit 4, Quantitative Tissue Distribution and Tertiary Structure Comparison of the ATPase Domain to RecA

Singh, Inderpal; Cai, Huicong; Wagner, B.J.

doi:10.1006/abbi.1996.0389

Cited by 4 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several of the cDNAs identified in the screen but not confirmed by QPCR to be differentially expressed, also belong to intriguing gene families with proven functions in the developing nervous system. These include another protein involved in selective protein degradation, ATPase complex subunit 4 (S4) one of several different subunits of the multimeric regulatory complex that combines with the 20S proteolytic core to form the 26S proteasome (Dubiel et al, 1992;Singh et al, 1996, Hutson et al, 1997. Regulation of cellular proliferation through ubiquitin-mediated proteolytic degradation of cell cycle components is a crucial function of the proteasome, and its levels are particularly high in immature and mitotically active cells (Ichihara and Tanaka, 1995).…”

Section: Discussionmentioning

confidence: 99%

Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia

Nelson

Sadhu

Kasemeier

et al. 2004

Developmental Dynamics

View full text Add to dashboard Cite

The dorsal root ganglia (DRG) derive from a population of migrating neural crest cells that coalesce laterally to the neural tube. As the DRG matures, discrete cell types emerge from a pool of differentiating progenitor cells. To identify genes that regulate sensory genesis and differentiation, we have designed screens to identify members from families of known regulatory molecules such as receptor tyrosine kinases, and generated full-length and subtractive cDNA libraries between immature and mature DRG for identifying novel genes not previously implicated in DRG development. Several genes were identified in these analyses that belong to important regulatory gene families. Quantitative PCR confirmed differential expression of candidate cDNAs identified from the subtraction/differential screening. In situ hybridization further validated dynamic expression of several cDNAs identified in our screens. Our results demonstrate the utility of combining specific and general screening approaches for isolating key regulatory genes involved in the genesis and differentiation of discrete cell types and tissues within the classic embryonic chick model system. Developmental Dynamics 229:618 -629, 2004.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia

Nelson

Sadhu

Kasemeier

et al. 2004

Developmental Dynamics

View full text Add to dashboard Cite

show abstract

“…The insert DNA was sequenced to confirm the deletion. The shortened insert then was transcribed in vitro to generate the internal standard RNA for the S7 fragment as described previously (Singh, Cai and Wagner, 1996). RNA was purified by phenol\chloroform extraction and precipitation.…”

Section: Molecular Cloning and Generating Internal Standard Rnas Of Smentioning

confidence: 99%

Gene Expression of the Proteasome in Rat Lens Development

Cai

Singh

Wagner

1998

Experimental Eye Research

Self Cite

View full text Add to dashboard Cite

Intracellular Proteinases of Invertebrates: Calcium-Dependent and Proteasome/Ubiquitin-Dependent Systems

Mykles

1998

International Review of Cytology

View full text Add to dashboard Cite

cDNA Cloning of a Chick Homologue of Human ATPase Complex Subunit 4, Quantitative Tissue Distribution and Tertiary Structure Comparison of the ATPase Domain to RecA

Cited by 4 publications

References 0 publications

Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia

Identification of genes regulating sensory neuron genesis and differentiation in the avian dorsal root ganglia

Gene Expression of the Proteasome in Rat Lens Development

Intracellular Proteinases of Invertebrates: Calcium-Dependent and Proteasome/Ubiquitin-Dependent Systems

Contact Info

Product

Resources

About