cDNA Cloning of Tropomyosin from the Anterior Byssus Retractor Muscle of Mussel and Its Structural Integrity from the Deduced Amino Acid Sequence

Isolation and characterization of a novel 45 kDa calponin-like protein from anterior byssus retractor muscle of the mussel Mytilus galloprovincialis

“…Two‐dimensional electrophoresis using an agarose gel for isoelectric focusing was adopted to detect proteins that constitute ABRM myofibrils. It showed major spots of myofibrillar proteins including myosin heavy chain, 17 paramyosin, 18 catchin, 19 actin, 17 tropomyosin, 20 and myosin light chains, 21 together with aggregated paramyosin, 18 as shown in Fig. 1.…”

cDNA cloning and deduced amino acid sequence of tropomyosin from fast skeletal muscle of white croaker Pennahia argentata

“…The amino acid sequences of these peptides are as follows: AMKDEEKMELQEIQLKEAKH (Ala 134 – His 153 ), HIAEEADRKYEEVARKLVI (His 153 –Ile 171 ), and KLVIIEGDLERTEERAELSESKCSE (Lys 168 –Glu 192 ). The number of amino acid residues coincides with those from most tropomyosins, 4,9,10,20,23,24 although the existence of shorter or longer molecules has also been reported 25,26 . White croaker skeletal muscle is considered to have only α‐type tropomyosin as do many other fish species 18 .…”

“…The tropomyosin molecule is composed of two subunits (approximately 34 000 Da), forming a two‐stranded coiled‐coil structure 1 . Knowledge to date indicate that tropomyosins possess an α‐helical structure throughout the molecule, with fundamental amino acid residue structural motifs in hydrophobic and hydrophilic arrangements 3–10 . In terms of the striated muscle system, this protein is one of the regulatory proteins that consist of thin filaments 11 .…”

Complete nucleotide sequences of cDNA encoding for tropomyosin isoforms from the catch muscle of scallop Patinopecten yessoensis

“…Comparison with sequences of other molluscan tropomyosins revealed that TM‐1, TM‐2 and TM‐3 had an identity of 100%, 92% and 95% to s‐tropomyosin; a 95%, 92% and 93% to akazara scallop tropomyosin; 7 and 71%, 72% and 72% to mussel retractor muscle tropomyosin, 8 respectively, at amino acid level. The high homology suggests that TM‐1, TM‐2 and TM‐3 are tropomyosin isoforms.…”

“…5,6 cDNA encoding for tropomyosin have already been isolated from molluscan muscles, such as the striated muscle of akazara scallop 7 and the anterior byssus retractor muscle of the mussel Mytilus edulis. 8 However, there have not been any reports about cDNA clones encoding tropomyosin isoforms of molluscan tissues.…”