cDNA microarray analysis reveals novel candidate genes expressed in human peripheral blood following exhaustive exercise

Zieker, Derek; Fehrenbach, Elvira; Dietzsch, Janko; Fliegner, Judith; Waidmann, Marc; Nieselt, Kay; Gebicke‐Haerter, Peter J.; Spanagel, Rainer; Simon, Perikles; Nieß, Andreas M.; Northoff, Hinnak

doi:10.1152/physiolgenomics.00096.2005

Cited by 67 publications

(71 citation statements)

References 54 publications

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“…These investigations (the FUNGENUT study) profiled gene expression in subcutaneous adipose tissue and found downregulation of IGF pathway genes and genes related to fat metabolism. Our results in prostate tissue were very similar; we also found down Recent studies also indicate that exercise may influence gene expression (42)(43)(44). These studies reported changes in gene expression in leukocytes after moderate or exhaustive exercise, with modulation of genes related to oxidative stress and inflammation.…”

Section: Discussionsupporting

confidence: 88%

Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention

Ornish

Magbanua

Weidner

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

267

152

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Epidemiological and prospective studies indicate that comprehensive lifestyle changes may modify the progression of prostate cancer. However, the molecular mechanisms by which improvements in diet and lifestyle might affect the prostate microenvironment are poorly understood. We conducted a pilot study to examine changes in prostate gene expression in a unique population of men with low-risk prostate cancer who declined immediate surgery, hormonal therapy, or radiation and participated in an intensive nutrition and lifestyle intervention while undergoing careful surveillance for tumor progression. Consistent with previous studies, significant improvements in weight, abdominal obesity, blood pressure, and lipid profile were observed (all P < 0.05), and surveillance of low-risk patients was safe. Gene expression profiles were obtained from 30 participants, pairing RNA samples from control prostate needle biopsy taken before intervention to RNA from the same patient's 3-month postintervention biopsy. Quantitative real-time PCR was used to validate array observations for selected transcripts. Two-class paired analysis of global gene expression using significance analysis of microarrays detected 48 up-regulated and 453 down-regulated transcripts after the intervention. Pathway analysis identified significant modulation of biological processes that have critical roles in tumorigenesis, including protein metabolism and modification, intracellular protein traffic, and protein phosphorylation (all P < 0.05). Intensive nutrition and lifestyle changes may modulate gene expression in the prostate. Understanding the prostate molecular response to comprehensive lifestyle changes may strengthen efforts to develop effective prevention and treatment. Larger clinical trials are warranted to confirm the results of this pilot study.exercise ͉ lifestyle changes ͉ prostate cancer ͉ SHOC2 ͉ stress management E pidemiological evidence (1, 2) and migrant studies (3) indicate that the incidence of clinically significant prostate cancer is much lower in parts of the world where people eat a predominantly low-fat, plant-based diet. We (4, 5) and others (6) have shown previously that diet and lifestyle interventions in men with earlystage prostate cancer decrease prostate-specific antigen (PSA) and decrease the rate of PSA increase. These studies provided some evidence that comprehensive lifestyle changes may have therapeutic potential in early prostate cancers. However, although these interventions are associated with decreased circulating insulin-like growth factor 1 (IGF1) (7), and although serum from men after intervention has reduced the ability to stimulate prostate cell-line growth in vitro (4), the actual molecular effects of these interventions in prostate tissue have not been previously examined.Many men with indolent prostate cancers detected by PSA screening will not exhibit disease progression during their lifetime; their treatment and associated side effects are unnecessary (8). We report here the results of the Gene Expressio...

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Section: Discussionsupporting

confidence: 88%

Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention

Ornish

Magbanua

Weidner

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

267

152

View full text Add to dashboard Cite

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“…In healthy subjects, aerobic conditioning increases the Th 1 response (8,26,42,65,68); however, its effect on the Th 2 response is poorly understood. Postulated immune mechanisms underlying allergic disease suggest that, in the general population, allergy should be less common in individuals with improved Th 1 response, since T lymphocytes would respond to antigen-presenting cells tending toward a Th 1 subtype.…”

Section: Discussionmentioning

confidence: 99%

“…Postulated immune mechanisms underlying allergic disease suggest that, in the general population, allergy should be less common in individuals with improved Th 1 response, since T lymphocytes would respond to antigen-presenting cells tending toward a Th 1 subtype. We then hypothesized that anti-inflammatory effect due to aerobic training in chronic allergic airway inflammation could be mediated by improved Th 1 response and consequent reduced Th 2 response (8,26,32,42,65,68). However, in our experimental animal model, the OVA-induced increase in the expression of Th 2 cytokines (IL-4 and IL-5) was reduced by aerobic training, although the expression of Th 1 cytokines (IL-2 and IFN-␥) remained unchanged, suggesting that the anti-inflammatory effects of aerobic training in a murine model of lung allergic inflammation may occur due to a direct effect on the Th 2 response.…”

Section: Discussionmentioning

confidence: 99%

“…Aerobic training has been shown to modulate the immune response in healthy individuals; however, its effect depends on training intensity (42). In general, moderate training intensity improves the immune system, observed by the increased neutrophil and macrophage phagocytosis capacity and a reduction in reactive oxygen species production, whereas high-intensity exercise leads to opposite effects (8,26,65,68). Aerobic exercise also has an important role in angiogenesis and reducing the vascular oxidative and remodeling process induced by cardiovascular disorders (5,23,64).…”

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confidence: 99%

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Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

Vieira¹,

Andrade²,

Duarte³

et al. 2008

American Journal of Physiology-Lung Cellular and Molecular Phys

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In addition, some studies demonstrated that aerobic training decreases chronic allergic inflammation in the airways; however, its effects on the pulmonary vessels and parenchyma have not been previously evaluated. Our objective was to test the hypothesis that aerobic conditioning reduces inflammation and remodeling in pulmonary vessels and parenchyma in a model of chronic allergic lung inflammation. Balb/c mice were sensitized at days 0, 14, 28, and 42 and challenged with ovalbumin (OVA) from day 21 to day 50. Aerobic training started on day 21 and continued until day 50. Pulmonary vessel and parenchyma inflammation and remodeling were evaluated by quantitative analysis of eosinophils and mononuclear cells and by collagen and elastin contents and smooth muscle thickness. Immunohistochemistry was performed to quantify the density of positive cells to interleukin (IL)-2, IL-4, IL-5, interferon-␥, IL-10, monocyte chemotatic protein (MCP)-1, nuclear factor (NF)-B p65, and insulin-like growth factor (IGF)-I. OVA exposure induced pulmonary blood vessels and parenchyma inflammation as well as increased expression of IL-4, IL-5, MCP-1, NF-B p65, and IGF-I by inflammatory cells were reduced by aerobic conditioning. OVA exposure also induced an increase in smooth muscle thickness and elastic and collagen contents in pulmonary vessels, which were reduced by aerobic conditioning. Aerobic conditioning increased the expression of IL-10 in sensitized mice. We conclude that aerobic conditioning decreases pulmonary vascular and parenchymal inflammation and remodeling in this experimental model of chronic allergic lung inflammation in mice.

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“…ABCA1 expression and abundance: The lymphocyte was powdered with cold mortar and pestle, and approximately 50 mg was used for the isolation of RNA. Total RNA was extracted by the guanidine thiocyanate method [25] and mRNA purified using an mRNA Isolation Kit (Roche, Germany) according to the manufacturer's instructions. Two-hundred nanograms of mRNA was used for synthesis of first strand cDNA by using oligo (dT) primer in the first-strand synthesis kit (Fermentase, Germany).…”

Section: Methodsmentioning

confidence: 99%

Effect of Eight Weeks of Wrestling and Circuit Fitness Training on APO Lipoprotein A-I and Lymphocyte ABCA1 Gene Expression in Well-Trained Wrestlers

Rashidlamir

Saadatnia

Ebrahimi-Atri

et al. 2011

International Journal of Wrestling Science

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ABSTRACT:Introduction: Atherosclerotic cardiovascular disease is now the leading cause of death in most countries. The ABCA1 gene provides instructions for the production of proteins (ATP-binding transporter protein) whose function is to export phospholipid and cholesterol out of the cells where they are bound to circulating apolipoprotein A1 (apoA1) and removed in the liver. Increased ABCA1 activity could inhibit atherosclerosis. In the present study, ABCA1 gene expression in lymphocytes and the associated effect of exercise were studied. Method: Subjects were 16 well-trained wrestlers randomly assigned into experimental and control groups. The experimental group performed 8 weeks of wrestling and circuit fitness training and the control group remained sedentary. Blood samples were collected 48 hours before the first session and 48 hours after the last session (subjects were fasting). After isolating lymphocytes by centrifugation, ABCA1 gene expression in lymphocytes was measured using semi-quantitative-RT-PCR. Data analyzed by SPSS software (version 16). Results: a significant increase in lymphocyte ABCA1 gene expression was shown following the 8 weeks of training (experimental p<0.001, t=-9.954). Plasma HDL-C concentrations and Apo A-I increased (P<0.001, t=4.97 P<0.05, t=2.67 respectively) and plasma LDL-C concentration decreased (P<0.001, t=4.35) in experimental group when compared with the control group. Discussion and Conclusion: Anaerobic exercises like wrestling and circuit fitness training can increase ABCA1 gene expression, an effective factor in the prevention of cardiovascular disease.

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cDNA microarray analysis reveals novel candidate genes expressed in human peripheral blood following exhaustive exercise

Abstract: . cDNA microarray analysis reveals novel candidate genes expressed in human peripheral blood following exhaustive exercise.

Cited by 67 publications

References 54 publications

Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention

Changes in prostate gene expression in men undergoing an intensive nutrition and lifestyle intervention

Aerobic conditioning and allergic pulmonary inflammation in mice. II. Effects on lung vascular and parenchymal inflammation and remodeling

Effect of Eight Weeks of Wrestling and Circuit Fitness Training on APO Lipoprotein A-I and Lymphocyte ABCA1 Gene Expression in Well-Trained Wrestlers

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