cDNA Sequence and Chromosomal Localization of the Remaining Three Human Nuclear Encoded Iron Sulphur Protein (IP) Subunits of Complex I: The Human IP Fraction Is Completed

Loeffen, Jan; Heuvel, Lambert van den; Smeets, Roel; Triepels, R.H.; Sengers, R. C. A.; Trijbels, Frans J.M.; Smeitink, Jan A.M.

doi:10.1006/bbrc.1998.8882

Cited by 22 publications

(16 citation statements)

References 45 publications

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“…31 The NDUFS2 protein is highly conserved in many species (95% in Bos taurus, 62% in Neurospora crassa, and 38% in Escherichia coli) which indicates its overall functional importance. 15 The NDUFS2 subunit also has weak homology with several proteins involved in the transport of protons, such as HycE (subunit of formate hydrogenlyase) and HyfG (subunit of Ni-Fe hydrogenase), so there might be an evolutionary relationship between these proteins. This implicates that there might be a role for the NDUFS2 subunit in proton transport.…”

Section: Discussionmentioning

confidence: 99%

“…14 Wildtype and patient NDUFS2 cDNA was amplified and sequenced. 15 All patients were screened for major mtDNA rearrangements by a previously developed long template PCR technique. 16 Furthermore, 11 common pathogenic mtDNA mutations were excluded in fibroblasts in our patient group by a combination of PCR and restriction fragment length polymorphism (RFLP).…”

Section: Mutational Analysismentioning

confidence: 99%

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Mutations in the complex INDUFS2 gene of patients with cardiomyopathy and encephalomyopathy

Loeffen¹,

Elpeleg

Smeitink³

et al. 2001

Ann Neurol.

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173

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Section: Discussionmentioning

confidence: 99%

Section: Mutational Analysismentioning

confidence: 99%

Mutations in the complex INDUFS2 gene of patients with cardiomyopathy and encephalomyopathy

Loeffen¹,

Elpeleg

Smeitink³

et al. 2001

Ann Neurol.

Self Cite

173

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“…NDUFS3, first identified by Jan Loeffen et al, 19 is one of the human nuclear-encoded iron-sulfur protein subunits of complex I. It functions after being transported from the cytoplasm to the inner-mitochondrial membrane.…”

Section: Discussionmentioning

confidence: 99%

“…16,17 Of those, ubiquinone oxidoreductase (complex I) is the largest complex. 18,19 NADH dehydrogenase (ubiquinone) Fe-S protein 3 (NDUFS3) is one of the nuclear gene-encoded precursor subunits in the 45-subunit mitochondrial complex I, and it is a key subunit for complex I assembly and activity. 20Y22 It was reported that the protein level of NDUFS3 was diminished in kidney tumor tissues compared with paired normal tissues, 23,24 and in the comparison of mitochondrial OXPHOS expression levels between human mammary carcinoma and human mammary epithelial cell, NDUFS3 expression was decreased in human mammary carcinoma.…”

mentioning

confidence: 99%

Reduced Expression of NDUFS3 and Its Clinical Significance in Serous Ovarian Cancer

Wang¹,

Cheng

Fu³

et al. 2013

International Journal of Gynecological Cancer

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“…No. NP_704816), Arabidopsis thaliana (At) (Welchen et al 2002) (Loeffen et al 1998), C. elegans (Ce) (acc. No.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of the pea cytochrome c oxidase Vb gene

Kubo

Arimura

Tsutsumi

et al. 2006

Genome

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Three copies of the gene that encodes cytochrome c oxidase subunit Vb were isolated from the pea (PscoxVb-1, PscoxVb-2, and PscoxVb-3). Northern Blot and reverse transcriptase-PCR analyses suggest that all 3 genes are transcribed in the pea. Each pea coxVb gene has an N-terminal extended sequence that can encode a mitochondrial targeting signal, called a presequence. The localization of green fluorescent proteins fused with the presequence strongly suggests the targeting of pea COXVb proteins to mitochondria. Each pea coxVb gene has 5 intron sites within the coding region. These are similar to Arabidopsis and rice, although the intron lengths vary greatly. A phylogenetic analysis of coxVb suggests the occurrence of gene duplication events during angiosperm evolution. In particular, 2 duplication events might have occurred in legumes, grasses, and Solanaceae. A comparison of amino acid sequences in COXVb or its counterpart shows the conservation of several amino acids within a zinc finger motif. Interestingly, a homology search analysis showed that bacterial protein COG4391 and a mitochondrial complex I 13 kDa subunit also have similar amino acid compositions around this motif. Such similarity might reflect evolutionary relationships among the 3 proteins.

show abstract

cDNA Sequence and Chromosomal Localization of the Remaining Three Human Nuclear Encoded Iron Sulphur Protein (IP) Subunits of Complex I: The Human IP Fraction Is Completed

Cited by 22 publications

References 45 publications

Mutations in the complex INDUFS2 gene of patients with cardiomyopathy and encephalomyopathy

Mutations in the complex INDUFS2 gene of patients with cardiomyopathy and encephalomyopathy

Reduced Expression of NDUFS3 and Its Clinical Significance in Serous Ovarian Cancer

Isolation and characterization of the pea cytochrome c oxidase Vb gene

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