1989
DOI: 10.1007/bf00036969
|View full text |Cite
|
Sign up to set email alerts
|

cDNA sequence and differential expression of the gene encoding the glutamine synthetase ? polypeptide ofPhaseolus vulgaris L.

Abstract: We report the sequence of an essentially full-length glutamine synthetase (GS) cDNA clone (pcGS-γ1) isolated from a root nodule library ofPhaseolus vulgaris L. The polypeptide encoded by this cDNA has been producedin vitro by transcription/translation and shown to co-migrate on two-dimensional gels with the previously identified major cytosolic GS polypeptide (γ) of nodules. Two previously identified GS cDNA clones, pR-2 and pR-1 (see Gebhardtet al., EMBO J 5: 1429-1435, 1986) have similarly been shown to enco… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

3
77
0
1

Year Published

1990
1990
2004
2004

Publication Types

Select...
6
4

Relationship

0
10

Authors

Journals

citations
Cited by 79 publications
(81 citation statements)
references
References 36 publications
3
77
0
1
Order By: Relevance
“…Recently, this was demonstrated for the nodule-specific glutamine synthetase gene. More detailed analyses showed that this gene is expressed at low levels in, for example, the stem of Phaseolus plants (Bennett et al, 1989). Also, the expression of a globin gene in both roots and nodules from Parasponia suggests that leghemoglobin, the "archetype" of the nodulins, might be expressed in nonsymbiotic tissues (Bogusz et al, 1988).…”
Section: Enodl2 Gene Expression In Stem and Flower Tissuementioning
confidence: 99%
“…Recently, this was demonstrated for the nodule-specific glutamine synthetase gene. More detailed analyses showed that this gene is expressed at low levels in, for example, the stem of Phaseolus plants (Bennett et al, 1989). Also, the expression of a globin gene in both roots and nodules from Parasponia suggests that leghemoglobin, the "archetype" of the nodulins, might be expressed in nonsymbiotic tissues (Bogusz et al, 1988).…”
Section: Enodl2 Gene Expression In Stem and Flower Tissuementioning
confidence: 99%
“…The pattern of distribution of cytosolic isoforms of GS and the expression of their genes vary among tissues and organs of individual plant species in response to the source of nitrogen and with the stage of development. For example, Phaseolus vulgaris has at least three genes for cytosolic GS that encode, respectively, the ␣-subunit found in leaves and roots, the ␤-subunit found in leaves, roots, and nodules, and the ␥-subunit, which is specific to nodules (5). Multiple forms of GS and the differential distribution of the corresponding mRNAs have also been reported in other species, including species of nonleguminous plants (6 -8).…”
mentioning
confidence: 99%
“…It has been proposed that GS 2 plays an important role in the assimilation of ammonia from nitrate reduction, and it has also been demonstrated that GS 2 is indispensable for re-assimilation of photorespiratory ammonia (Blackwell et al 1987;Wallsgrove et al 1987;Kozaki and Takeba 1996). GS 1 is encoded by a small multigene family, and the different GS 1 isoforms are differentially regulated in a tissue-and developmental stage-specific manner (Bennett et al 1989;Peterman and Goodman 1991;Temple et al 1995;Dubois et al 1996;Morey et al 2002). GS 1 plays a major role in the assimilation of ammonia derived from N 2 fixation in the nodules and also has an important role in the remobilization of nitrogen during senescence, bacterial infection, herbicide treatment and water stress (Bauer et al 1997;Pérez-García et al 1998;Brugiere et al 2000;Masclaux et al 2000).…”
Section: Introductionmentioning
confidence: 99%