cDNA sequence and tissue expression analysis of glucokinase from liver of grass carp (Ctenopharyngodon idella)

Cheng, Hanliang; Ji, Nan-jing; Peng, Yong-xing; Shen, Xin; Xu, Jianhong; Dong, Zhiguo; Chen-chen, WU

doi:10.5897/ajb11.3174

Cited by 1 publication

(1 citation statement)

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“…Even if unexpectedly downregulated by the nutritional status, the detection of pfkla and pklr transcripts in the telencephalon signed for a glycolytic potential of the brain in the surface A. mexicanus, as previously described in several teleost species regardless of their nutritional preferences [50][51][52]. Surprisingly, no gck mRNA was detected in the brain, although it has been demonstrated to exist in several fish species [53][54][55]. Moreover, by the detection of fbp1a and pck2 mRNA in the telencephalon, one cannot exclude the presence of gluconeogenesis pathway in the brain of the species, even though the present study may be in the future completed by enzymatic activity analysis.…”

Section: Brain: Central Glucose and Nutrient Sensingsupporting

confidence: 56%

Nutritional regulation of glucose metabolism-related genes in the emerging teleost model Mexican tetra surface fish: a first exploration

et al. 2020

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Astyanax mexicanus has gained importance as a laboratory model organism for evolutionary biology. However, little is known about its intermediary metabolism, and feeding regimes remain variable between laboratories holding this species. We thus aimed to evaluate the intermediary metabolism response to nutritional status and to low (NC) or high (HC) carbohydrate diets in various organs of the surface-dwelling form of the species. As expected, glycaemia increased after feeding. Fish fed the HC diet had higher glycaemia than fish fed the NC diet, but without displaying hyperglycaemia, suggesting that carbohydrates are efficiently used as an energy source. At molecular level, only fasn ( Fatty Acid Synthase ) transcripts increased in tissues after refeeding, suggesting an activation of lipogenesis. On the other hand, we monitored only moderate changes in glucose-related transcripts. Most changes observed were related to the nutritional status, but not to the NC versus HC diet. Such a metabolic pattern is suggestive of an omnivorous-related metabolism, and this species, at least at adult stage, may adapt to a fish meal-substituted diet with high carbohydrate content and low protein supply. Investigation to identify molecular actors explaining the efficient use of such a diet should be pursued to deepen our knowledge on this species.

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