CdS nanocrystals/graphene oxide-AuNPs based electrochemiluminescence immunosensor in sensitive quantification of a cancer biomarker: p53

Heidari, Reza; Rashidiani, Jamal; Abkar, Morteza; Taheri, Ramezan Ali; Moghaddam, Mehrdad Moosazadeh; Mirhosseini, Seyed Ali; Seidmoradi, Rezvan; Nourani, Mohammad Reza; Mahboobi, Mahdieh; Keihan, Amir Homayoun; Kooshki, Hamid

doi:10.1016/j.bios.2018.10.031

Cited by 67 publications

(29 citation statements)

References 24 publications

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“…These peaks are unique to CdS nanoparticles synthesized in our paper. Also, these data are consistent with other literatures [25,32]. AuNPs, str-AuNPs and str-Lum-AuNPs authenticity were evaluated by different methods.…”

Section: Characterization Of the Materialssupporting

confidence: 91%

“…Consequently, 8.5 was considered as the optimized pH. These data and conclusions here are consistent with other literatures [3,25,37].…”

Section: Finding the Optimum Ph Valuesupporting

confidence: 89%

“…Since the ECL responses of the CdS and luminol are dependent on the H 2 O 2 concentration in PBS, we carried out the experiments to evaluate and obtain the optimal H 2 O 2 concentration. To do this, in QM and AM, the electrode was prepared using 1000 pg.mL −1 thrombin and used as the working electrode in a PBS (0.1 M, pH = 7.5) with different concentrations of H 2 O 2 (5,10,15,20,25,30,35,40 and 50 mM) at 0.1 V. s −1 . The resulted ECL intensities and peaks are presented in Supplementary material, Fig.…”

Section: H 2 O 2 Concentration Optimizationmentioning

confidence: 99%

“…ECL of the CdS induces the SPR of AuNPs, which in return, amplifies the ECL response of CdS [13]. In this CdS-AuNPs group, CdS is ECL donor and AuNPs are ECL acceptors [25,26].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study

et al. 2019

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The detection of thrombin by using CdS nanocrystals (CdS NCs), gold nanoparticles (AuNPs) and luminol is investigated in this work. Thrombin is detected by three methods. One is called the quenching method. It is based on the quenching effect of AuNPs on the yellow fluorescence of CdS NCs (with excitation/emission wavelengths of 355/550 nm) when placed adjacent to CdS NCs. The second method (called amplification method) is based on an amplification mechanism in which the plasmonics on the AuNPs enhance the emission of CdS NCs through distance related Förster resonance energy transfer (FRET). The third method is ratiometric and based on the emission by two luminophores, viz. CdS NCs and luminol. In this method, by increasing the concentration of thrombin, the intensity of CdS NCs decreases, while that of luminol increases. The results showed that ratiometric method was most sensitive (with an LOD of 500 fg.mL-1), followed by the amplification method (6.5 pg.mL-1) and the quenching method (92 pg.mL-1). Hence, the latter is less useful.

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Section: Characterization Of the Materialssupporting

confidence: 91%

“…Consequently, 8.5 was considered as the optimized pH. These data and conclusions here are consistent with other literatures [3,25,37].…”

Section: Finding the Optimum Ph Valuesupporting

confidence: 89%

Section: H 2 O 2 Concentration Optimizationmentioning

confidence: 99%

“…ECL of the CdS induces the SPR of AuNPs, which in return, amplifies the ECL response of CdS [13]. In this CdS-AuNPs group, CdS is ECL donor and AuNPs are ECL acceptors [25,26].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study

et al. 2019

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“…Measurement of electrochemiluminescence (ECL) is an outstanding method for sensing applications [14,15], which has been widely used in drug analysis [16], amino acid analysis [17], DNA probe analysis [18] and the field of enzyme biosensors [19]. Ru(bpy) 3 2+ is a common luminophore in ECL measurements [20,21]. Amines can be employed as reducing agent for Ru(bpy) 3 2+ , such as tripropylamine [22,23] and melamine [24].…”

Section: Introductionmentioning

confidence: 99%

Novel Detection of Acrylamide by Electrochemiluminescence Sensor and Optical Imaging Analysis

Yang¹

2019

International Journal of Electrochemical Science

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Acrylamide, a carcinogen with debilitating effects on human health, was detected by electrochemiluminescence (ECL) measurement using Ru(bpy)3 2+ as the luminophore. Under the optimum conditions, Pt electrode as the working electrode showed a superior linear detection in the range 5 μM-10 mM, with a detection limit of 1.2 µM (S/N = 3). Moreover, a remote wireless camera was employed to collect the digital images of the ECL emission. These images were converted from RGB (Red, Green, Blue) model to HSV (Hue, Saturation, Value) model using the MATLAB software. It was found that Value (V) in the HSV model had a linear relationship with the logarithm of the acrylamide concentration, suggesting that acrylamide could be detected in levels of 5 μM using optical imaging. The ECL method for detecting acrylamide was proved to be simple and convenient. This is expected to lead to the development of a novel acrylamide detection method for food safety and other fields.

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Antibody‐Based Biosensors

2024

Biosensors

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CdS nanocrystals/graphene oxide-AuNPs based electrochemiluminescence immunosensor in sensitive quantification of a cancer biomarker: p53

Cited by 67 publications

References 24 publications

Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study

Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study

Novel Detection of Acrylamide by Electrochemiluminescence Sensor and Optical Imaging Analysis

Antibody‐Based Biosensors

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