CdSe/ZnS Quantum Dots trigger DNA repair and antioxidant enzyme systems in Medicago sativacells in suspension culture

Santos, Ana R; Miguel, Ana Sofia; Macovei, Anca; Maycock, Christopher D.; Balestrazzi, Alma; Oliva, Abel; Fevereiro, Pedro

doi:10.1186/1472-6750-13-111

Cited by 27 publications

(13 citation statements)

References 51 publications

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“…Additional work provided further information on the possible involvement of the Tdp1β gene in the plant stress response. Santos et al [ 5 ] showed the upregulation of Tdp1β gene in Medicago sativa suspension cultures exposed to genotoxic doses of CdSe/ZnS quantum dots, highlighting a strong correlation with DNA damage accumulation/repair kinetics. An RNA-Seq analysis carried out in MtTdp1α -depleted M. truncatula plants revealed that the MtTdp1β gene does not play a redundant function since the β isoform was not able to compensate for the lack of MtTdp1α gene in planta [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

Sabatini¹,

Pagano²,

Araújo

et al. 2017

Genes

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Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is involved in DNA repair pathways as it mends the topoisomerase I—DNA covalent complexes. In plants, a small Tdp1 gene family, composed by Tdp1α and Tdp1β genes, was identified, but the roles of these genes in abiotic stress responses are not fully understood. To investigate their specific stress response patterns, the present study made use of bioinformatic and molecular tools to look into the Tdp1β gene function, so far described only in the plant kingdom, and compare it with Tdp1α gene coding for the canonical, highly conserved α isoform. The expression profiles of Tdp1α and Tdp1β genes were examined under abiotic stress conditions (cold, heat, high osmolarity, salt, and UV-B) in two model species, Arabidopsis thaliana and Medicago truncatula. The two isoforms of topoisomerase I (TOP1α and TOP1β) were also taken into consideration in view of their known roles in DNA metabolism and cell proliferation. Data relative to gene expression in Arabidopsis were retrieved from the AtGenExpress microarray dataset, while quantitative Real-Time PCR was carried out to evaluate the stress response in M. truncatula cell cultures. These analyses revealed that Tdp1β gene expression was enhanced during the first hour of treatment, whereas Tdp1α enhanced expression succeeded at subsequent timepoints. In agreement with the gene-specific responses to abiotic stress conditions, the promoter regions of Tdp1α and Tdp1β genes are well equipped with stress-related cis-elements. An in-depth bioinformatic characterization of the HIRAN motif, a distinctive feature of the Tdp1β protein, showed its wide distribution in chromatin remodeling and DNA repair proteins. The reported data suggests that Tdp1β functions in the early response to abiotic stresses.

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Section: Introductionmentioning

confidence: 99%

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

Sabatini¹,

Pagano²,

Araújo

et al. 2017

Genes

Self Cite

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“…Despite their increasing prevalence in the environment, the toxicity of quantum dots in plants is largely unknown. In a pioneer study, Santos et al ( 2013 ) used a battery of tests and gene expression related with DNA repair, and demonstrated that 10 nM 3-mercaptopropanoic coated-CdSe/ZnS quantum dots were cytotoxic and genotoxic to Medicago sativa cells. In this and other pioneer studies, the comet assay can play a pivotal role as a tool to assess environmental impacts of suspected emerging nanocontaminants.…”

Section: Nanocompoundsmentioning

confidence: 99%

“…Santos et al ( 2013 ) demonstrated in Medicago sativa that exposure to increasing concentrations of MPA-CdSe/ZnS quantum dots, led to an increase of DNA damages, and up-regulated the transcription of the DNA repair enzymes formamidopyrimidine DNA glycosylase, tyrosyl-DNA phosphodiesterase I and DNA topoisomerase I.…”

Section: Comet Assay and Putative Genetic Associationsmentioning

confidence: 99%

The use of comet assay in plant toxicology: recent advances

2015

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The systematic study of genotoxicity in plants induced by contaminants and other stress agents has been hindered to date by the lack of reliable and robust biomarkers. The comet assay is a versatile and sensitive method for the evaluation of DNA damages and DNA repair capacity at single-cell level. Due to its simplicity and sensitivity, and the small number of cells required to obtain robust results, the use of plant comet assay has drastically increased in the last decade. For years its use was restricted to a few model species, e.g., Allium cepa, Nicotiana tabacum, Vicia faba, or Arabidopsis thaliana but this number largely increased in the last years. Plant comet assay has been used to study the genotoxic impact of radiation, chemicals including pesticides, phytocompounds, heavy metals, nanoparticles or contaminated complex matrices. Here we will review the most recent data on the use of this technique as a standard approach for studying the genotoxic effects of different stress conditions on plants. Also, we will discuss the integration of information provided by the comet assay with other DNA-damage indicators, and with cellular responses including oxidative stress, cell division or cell death. Finally, we will focus on putative relations between transcripts related with DNA damage pathways, DNA replication and repair, oxidative stress and cell cycle progression that have been identified in plant cells with comet assays demonstrating DNA damage.

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“…Metal sulphide nanomaterials have gained more attention in the field of cancer therapeutics due to their reactive oxygen species (ROS) generating and apoptosis inducing ability. [ 20–22 ] Compared with other metal sulphide nanoparticles, ZnS NPs have a higher surface‐to‐volume ratio, quantum size effect, quantum tunnelling effect, optical absorption, chemical activity, catalysis, and thermal resistance properties, therefore rendering them with various chemical, optical and biological applications like biosensors, drug delivery, photocatalysis, and theranostics. [ 23 ] Recently, ZnS NPs have been shown to induce cytotoxic effects in leukaemic and retinal pigment epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

Molecular interaction studies of zinc sulphide nanoparticles with DNA and its consequence: a multitechnique approach

2020

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Molecular interaction studies between nanoparticles (NPs) and biomolecules are of great importance in the field of nanomedicine as they affect many physiological processes. Therefore, the interaction of zinc sulphide nanoparticles (ZnS NPs) with calf thymus deoxyribonucleic acid (CT DNA) and its significance was analyzed using ultraviolet (UV)–visible light, fluorescence, circular dichroism (CD), zeta potential, viscometry, electrochemical, and polymerase chain reaction methods. Fluorescence quenching analysis revealed that the fluorescence of ZnS NPs was quenched using CT DNA through a static quenching mechanism. The negative values of thermodynamic parameters (ΔG, ΔH, and ΔS) showed that the binding process was spontaneous, exothermic, and van der Waals or hydrogen bonding plays an important role in the interaction of ZnS NPs with CT DNA. Thermal melting (Tm) studies indicated a decrease in the Tm of CT DNA, suggesting the destabilization of CT DNA upon interaction with ZnS NPs. In addition, the results obtained from competitive binding, zeta potential, CD, and viscometry measurements showed that the interaction of ZnS NPs with CT DNA is through groove binding. Electrochemical analysis further confirmed the observed results from various spectroscopic and other related studies, in which decrease in the redox peak current along with changes in peak potential (CV) and increase in the electrical resistance (EIS) indicated the interaction between ZnS NPs and CT DNA. Furthermore, PCR analysis using DNA polymerase revealed the potential of ZnS NPs to inhibit DNA replication in vitro. ZnS NP–CT DNA interaction studies can be explored to define new horizons in biomedical applications of ZnS NPs.

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CdSe/ZnS Quantum Dots trigger DNA repair and antioxidant enzyme systems in Medicago sativacells in suspension culture

Cited by 27 publications

References 51 publications

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

The use of comet assay in plant toxicology: recent advances

Molecular interaction studies of zinc sulphide nanoparticles with DNA and its consequence: a multitechnique approach

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