CdTe quantum dots linked to Glutathione as a bridge for protein crosslinking

Beato-López, J.J.; Espinazo, M.L.; Fernández-Ponce, Cecilia; Blanco, E.; Ramírez‐del‐Solar, M.; Domínguez, M.; García-Cózar, Francisco; Litrán, R.

doi:10.1016/j.jlumin.2017.03.012

Cited by 12 publications

(12 citation statements)

References 30 publications

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“…After 30 additional minutes, the red wine color solution was stored at 8 °C protected from light. Based on our previous works (Beato-Lopez et al 2017 ; Beato-Lopez et al 2012 ), we have designed this rapid route to obtained small GSH capped gold NPs. Figure 1 c shows a schematic representation of the final NP obtained by this synthesis.…”

Section: Methodsmentioning

confidence: 99%

“…For the citrate-based method, we have used variations of the traditional Turkevich route as well as a “seeded-growth” method, introducing simplifications on the Piella et al route (Piella et al 2016 ). For the reduction with sodium borohydride, we used either cysteamine (C 2 H 7 NS (CYS)) (Niidome et al 2004 ) or glutathione (C 10 H 17 N 3 O 6 S (GSH)) (Beato-Lopez et al 2017 ; Beato-Lopez et al 2012 ) as capping agents. Both capping agents contain a thiol group to be linked to the NP surface, as well as an amino terminal group (in the case of CYS) or both amino and carboxylic groups (in the case of GSH), that remain free to be cross-linked with other biomolecules, as we have previously shown (Beato-Lopez et al 2017 ).…”

Section: Introductionmentioning

confidence: 99%

“…For the reduction with sodium borohydride, we used either cysteamine (C 2 H 7 NS (CYS)) (Niidome et al 2004 ) or glutathione (C 10 H 17 N 3 O 6 S (GSH)) (Beato-Lopez et al 2017 ; Beato-Lopez et al 2012 ) as capping agents. Both capping agents contain a thiol group to be linked to the NP surface, as well as an amino terminal group (in the case of CYS) or both amino and carboxylic groups (in the case of GSH), that remain free to be cross-linked with other biomolecules, as we have previously shown (Beato-Lopez et al 2017 ). Thus, starting from different synthetic routes, we obtain NPs with different capping, size, and homogeneity (Albanese et al 2012 ; Chithrani et al 2006 ; Li et al 2018 ; Silvestri et al 2017 ; Verma and Stellacci 2010 ).…”

Section: Introductionmentioning

confidence: 99%

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Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

et al. 2018

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Hydrophilic and homogeneous sub-10 nm blue light-emitting gold nanoparticles (NPs) functionalized with different capping agents have been prepared by simple chemical routes. Structure, average, size, and surface characteristics of these NPs have been widely studied, and the stability of colloidal NP solutions at different pH values has been evaluated. Au NPs show blue PL emission, particularly in the GSH capped NPs, in which the thiol-metal core transference transitions considerably enhance the fluorescent emission. The influence of capping agent and NP size on cytotoxicity and on the fluorescent emission are analyzed and discussed in order to obtain Au NPs with suitable features for biomedical applications. Cytotoxicity of different types of gold NPs has been determined using NPs at high concentrations in both tumor cell lines and primary cells. All NPs used show high biocompatibility with low cytotoxicity even at high concentration, while Au-GSH NPs decrease viability and proliferation of both a tumor cell line and primary lymphocytes.Electronic supplementary materialThe online version of this article (10.1007/s11051-018-4406-0) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

et al. 2018

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“…Even in the case of Quantum dots (QDs) with a potential toxic core, we concluded that there was no significative increase in cellular death when NP was present, due to the GSH entirely covers the surface of the NP. On the other hand, we have also reported the efficiency of GSH capping demonstrating that, after capping, the GSH molecule retains the capability of linking to biological species and also the ability for specifically links [35,36].…”

Section: Introductionmentioning

confidence: 94%

“…These TEM NPs sizes can be used for the estimation of the number of GSH molecules surrounding the magnetic core. With this purpose, a simple model described elsewhere [35] is employed. This model is based on the assumption of a spherical Fe 3 O 4 core covered by a single layer of GSH molecules linked through the thiol group and forming a spherical crown of variable density.…”

Section: Structural Characterizationmentioning

confidence: 99%

Glutathione-magnetite nanoparticles: synthesis and physical characterization for application as MRI contrast agent

et al. 2020

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In this work a one-pot synthesis of water soluble glutathione capped magnetite nanoparticles is reported. The magnetic characterization of the samples shows the expected superparamagnetic behavior, but a wide range of blocking temperatures is found, since the size and interparticle interactions are very sensitive to preparation conditions. These properties are correlated with the glutathione-iron ratio and oxidant dose, in order to optimize the aqueous colloidal stability and magnetic properties of the superparamagnetic iron oxide nanoparticles for Magnetic Resonance Imaging applications. The efficiency of the glutathione coated nanoparticles as contrast agent is then evaluated by means of the determination of the relaxation times T1 and T2 in 1 H Nuclear Magnetic Resonance experiments. Moreover, the influence of the thickness of the glutathione capping layer on the colloidal stability and, thus, on relaxation times has been studied. Finally, the relaxitivity of the sample that shows the best performance has been determined. Graphic abstract A novel one-pot synthesis for "in situ" functionalized hydrophilic magnetite nanoparticles at atmospheric pressure and a temperature lower than 200 °C for Magnetic Resonance Imaging applications is reported. Their properties are analyzed in terms of the synthesis process: glutathione-iron ratio and oxidant dose.

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(S)TEM structural and compositional nanoanalyses of chemically synthesized glutathione-shelled nanoparticles

et al. 2020

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This work is focused on the characterization by transmission and scanning-transmission electron microscopy-related techniques of core-shell nanoparticles synthesized via chemical methods. Diferent semiconducting, pure metallic or oxide materials have been utilized as the core (cadmium telluride, gold, magnetite, or magnetite covered with gold) of the nanoparticle, while they have been, in all cases, functionalized by a thin amorphous glutathione layer, with the goal of using the nanoparticles in biomedical applications such as biomarkers, and computerized tomography and image magnetic resonance contrast agents. The results show that it is possible to visualize the glutathione layer using spectroscopic and imaging techniques, associated with electron microscopy (such as energy-dispersive X-ray spectroscopy and high-resolution transmission electron microscopy images), that this layer is present at the surfaces of all observed nanoparticles, and that it is no thicker than a few nanometers. Electron microscopy also revealed that the nanoparticles core is crystalline and, in average, around 5-nm size.

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CdTe quantum dots linked to Glutathione as a bridge for protein crosslinking

Cited by 12 publications

References 30 publications

Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

Influence of size and surface capping on photoluminescence and cytotoxicity of gold nanoparticles

Glutathione-magnetite nanoparticles: synthesis and physical characterization for application as MRI contrast agent

(S)TEM structural and compositional nanoanalyses of chemically synthesized glutathione-shelled nanoparticles

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