Cecal Ligation and Incision: An Acute Onset Model of Severe Sepsis in Rats

Scheiermann, Patrick; Hoegl, Sandra; Revermann, Marc; Ahluwalia, Devan; Zander, Johannes; Boost, Kim Alexander; Nguyen, T.H.; Zwißler, Bernhard; Mühl, Heiko; Hofstetter, Christian

doi:10.1016/j.jss.2008.02.032

Cited by 35 publications

(33 citation statements)

References 38 publications

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“…In contrast, 300 min after induction of the CLI sepsis, only plasma concentrations of IL-1β and IL-6 are elevated but are not as high as in endotoxemia. Plasma levels of IL-1β and IL-6 have been shown to be significantly induced in rats 390 min after sepsis induction [11,12]. In the present study, the observation time was limited to 300 min after the CLI procedure.…”

Section: Discussionmentioning

confidence: 89%

“…In contrast, proinflammatory cytokine levels (including TNF-α) are markedly higher 300 min after LPS injection than after CLI sepsis. This immediate induction of a robust proinflammatory response is an unquestionable advantage of the LPS-based approach and will limit the use of the CLI sepsis model to an observation time of at least 390 min [11][12][13][14]. Nevertheless, only the CLI procedure provides a polymicrobial insult, which is also an essential characteristic of severe sepsis.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, the ligated cecum was opened through a 1.5 cm blade incision (No. 10, Feather, Osaka, Japan) on the anti-mesenteric side as described before [11]. The cecum was then carefully replaced into the abdomen.…”

Section: Experimental Protocol and Surgical Proceduresmentioning

confidence: 99%

“…In order to fill this gap, we have recently published an acute sepsis model induced by cecal ligation and incision (CLI) [11], which has …”

Section: Introductionmentioning

confidence: 99%

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Comparing hemodynamics, blood gas analyses and proinflammatory cytokines in endotoxemic and severely septic rats

Scheiermann

Hoegl

Hofstetter

et al. 2011

International Immunopharmacology

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Experimental Protocol and Surgical Proceduresmentioning

confidence: 99%

“…In order to fill this gap, we have recently published an acute sepsis model induced by cecal ligation and incision (CLI) [11], which has …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparing hemodynamics, blood gas analyses and proinflammatory cytokines in endotoxemic and severely septic rats

Scheiermann

Hoegl

Hofstetter

et al. 2011

International Immunopharmacology

Self Cite

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“…Impaired absorption of inflammatory mediators from the peritoneal cavity warrants further investigation in a Gram-positive, Gram-negative and mixed bacterial peritonitis [2,28]. …”

Section: Discussionmentioning

confidence: 99%

Peritoneal Resorption Capacity for Lipopolysaccharide and Interleukin-6 in Acute Zymosan-Induced Chemical Peritonitis

Torab¹,

Abu‐Zidan²,

Al-Salam³

et al. 2011

Eur Surg Res

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Aim: To evaluate peritoneal resorption capacity for lipopolysaccharide (LPS) and interleukin-6 (IL-6) in a model of chemical peritonitis. Methods: Zymosan peritonitis was induced in anesthetized rats. LPS was injected intraperitoneally to different groups at 4 h (n = 10), 8 h (n = 9), 12 h (n = 9), and 24 h (n = 9) after peritonitis and to a control group (n = 8). Similarly, IL-6 was injected intraperitoneally to different groups at 4 h (n = 9), 8 h (n = 10), 12 h (n = 10), and 24 h (n = 10) after peritonitis, and to a control group (n = 10). Plasma levels of LPS or IL-6 were measured immediately after intraperitoneal injections of LPS or IL-6, respectively, and at 5, 15, 30, 45, and 60 min later. Results: There was no change over time in plasma LPS levels in the groups receiving LPS intraperitoneally (p = 0.4). There was highly significant change over time in the IL-6 level in the studied time periods in the groups receiving IL-6 intraperitoneally (p < 0.0001). There was an increase in the plasma IL-6 level when sampled at 4 h after peritonitis. Conclusion: There was a reduction of resorption capacity of inflamed peritoneum for inflammatory mediators in acute chemical peritonitis.

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Interleukin-6 Contributes to the Paracrine Effects of Cardiospheres Cultured from Human, Murine and Rat Hearts

et al. 2014

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Cardiosphere-derived cells (CDCs) were cultured from human, murine, and rat hearts. Diluted supernatant (conditioned-medium) of the cultures improved the contractile behavior of isolated rat cardiomyocytes (CMCs). This effect is mediated by the paracrine release of cytokines. The present study tested the hypothesis, that the cardiovascular state of the donor's heart influences this effect on CMCs and tries to identify the responsible factors. CDCs were cultured from human tissue samples of cardiac surgery and from murine and rat hearts. The supernatants of cultured CDCs from hypertensive humans and rats showed a higher improvement of the contractile behavior of CMCs compared to CDCs of normotensive origin. Subsequently, the cytokine profile of the supernatants was analyzed. Among the cytokines elevated in supernatants originating from hypertensive humans or rats was Interleukin-6. CDCs were also generated from Interleukin-6(-/-) -mice and their wildtype littermates. The supernatant of the cultured Interleukin-6(-/-) -CDCs had no effect on the contractile behavior, whereas the supernatant of the Interleukin-6(+/+) -CDCs showed a positive effect. To confirm the hypothesis that Interleukin-6 contributes to the paracrine effects, CMCs were incubated with Interleukin-6. It improved the contractile function in a concentration dependent way. Finally, the effect of the supernatant of cultured CDCs derived from a hypertensive human sample could be abolished by simultaneous incubation with a specific Interleukin-6 antibody. CDCs release cytokines that improve the contractile behavior of CMCs. This effect is more intense in CDCs from hypertensive donors. Interleukin-6 is involved in this phenomenon.

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Cecal Ligation and Incision: An Acute Onset Model of Severe Sepsis in Rats

Cited by 35 publications

References 38 publications

Comparing hemodynamics, blood gas analyses and proinflammatory cytokines in endotoxemic and severely septic rats

Comparing hemodynamics, blood gas analyses and proinflammatory cytokines in endotoxemic and severely septic rats

Peritoneal Resorption Capacity for Lipopolysaccharide and Interleukin-6 in Acute Zymosan-Induced Chemical Peritonitis

Interleukin-6 Contributes to the Paracrine Effects of Cardiospheres Cultured from Human, Murine and Rat Hearts

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