Cefoselis enhances breast cancer chemosensitivity by directly targeting GRP78/LRP5 signalling of cancer stem cells

Multidimensional pan-cancer analysis of HSPA5 and its validation in the prognostic value of bladder cancer

“…At the same time, Cancer development is promoted by a coordinated set of immune regulatory systems [ 24 ]. HSPA5, as an ER stress-related gene, is highly expressed in various malignant tumor tissues such as colorectal [ 25 ], liver [ 26 ], and breast cancers [ 27 ], which may lead to malignant biological behaviors such as proliferation, invasion, induction of chemotherapy resistance and immune escape of tumor cells. However, there is a lack of thorough investigations of the HSPA5 functional role to highlight the similarities and variations between various malignancies that would provide key insights to bolster cancer prevention and develop individualized treatment plans.…”

“…Cell surface GRP78-targeted CAR-T cells have also been shown to be effective in treating human pancreatic cancer [ 14 ]. In addition, HSPA5 has been shown to affect the stemness profile of cancer cells in esophageal, glioblastoma, gastric and breast cancers [ [15] , [16] , [17] , [18] ].…”

Baohuoside I chemosensitises breast cancer to paclitaxel by suppressing extracellular vesicle/CXCL1 signal released from apoptotic cells

“…CETSA assay was performed as previously described (Zheng et al., 2023 ) to investigate the thermal stability change of FLOT2 in 4T1 cell lysate after BHS treatment. Briefly, 4T1 cells were treated with 5 µM BHS for 3 h. Then, the BHS‐treated and untreated 4T1 cells were harvested, freeze‐thawed and lysed.…”

“…The 3D structure of FLOT2 was acquired from an AlphaFold structure prediction (Jumper et al., 2021 ; Varadi et al., 2022 ) and the binding site was set on the SPFH domain of FLOT2 since the 104 LQTL 107 motif in the SPFH domain of FLOT2 is responsible for FLOT2 interactions (Wei et al., 2021 ). Flexible docking of BHS to FLOT2 and the subsequent virtual alanine scan protocols were performed as previously described (Zheng et al., 2023 ). Briefly, the BHS molecule structure was prepared and minimized before the flexible docking protocol was performed.…”