Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant Escherichia coli O157:H7 and O55:H7 in Camel Meat

Sallam, Khalid Ibrahim; Abd-Elrazik, Yasmine; Raslan, Mona Talaat; Imre, K.; Morar, Adriana; Herman, V.; Zaher, Hanan A.

doi:10.3390/foods12071443

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…In Egypt, E. coli O26, O86, O111, O124, and O128 were isolated from camel meat slaughtered in Elbagour, Menouf, and Shibin Elkom slaughter houses at a prevalence rate of 30%, 20%, and 15%, respectively ( Edris et al ., 2013 ). Multidrug-resistant E. coli was also isolated from camel meat in Mansoura city ( Sallam et al ., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence and antibiogram of shigatoxin-producing E. coli in camel meat and offal

Elkady,

Darwish,

Tharwat

et al. 2024

Open Vet J

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Background: Camels are important animals in Egypt and other Arab countries on the basis of the economic value and ethnic culture. E. coli is implicated in several gastrointestinal infections and outbreaks worldwide, especially in developing counties. It causes infections that might lead to death. Numerous biological activities, such as antioxidative, antibacterial, anti-diabetic, anti-mutagenic, anti-inflammatory, neuroprotective, and diuretic, are associated with coriander and coriander essential oils. Aim: This work targeted investigation of the prevalence, antibiogram, and occurrence of virulence genes of E. coli in camel meat liver, and kidney. Besides, the anti-E. coli activity of coriander oil was further examined. Methods: Camel meat, liver and kidneys were collected from local markets of Egypt. Isolation and identification of E. coli were performed. Antimicrobial susceptibility of the obtained E. coli isolates was screened using the disk diffusion assay. To detect presence of virulence-associated genes (stx1, stx2, eaeA and hylA gens), polymerase chain reaction (PCR) was used. Experimental trial was done to investigate the anti-E. coli activity of coriander oil. Results: The obtained results revealed isolation of the following E. coli pathotypes: O17:H18, O128:H2, O119:H6, O103:H4, O145:H-, and O121:H7. The recovered E. coli isolates practiced multidrug resistance profiling with higher resistance towards Erythromycin, Nalidixic Acid, Clindamycin, and Ampicillin. But the isolates were sensitive to Meropenem, Cefoxitin. The recovered isolates had expressed different virulence attributes. Coriander oil 2% could significantly reduce E. coli O128 count in camel meat by 65%. Conclusion: Therefore, strict hygienic measures are highly recommended during processing of camel meat. The use of coriander oil during camel meat processing is highly recommended to reduce E. coli count.

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Section: Discussionmentioning

confidence: 99%

Prevalence and antibiogram of shigatoxin-producing E. coli in camel meat and offal

Elkady,

Darwish,

Tharwat

et al. 2024

Open Vet J

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“…Khalid et al 20 study in Egypt from the period of November 2021 to March 2022 reported that meat marketed in Beheira Governorate, Egypt, was contaminated with multi and extensively drug resistant Shiga toxigenic E. coli O157:H7 with a prevalence rate 9.1%; 10 out of 110 testes samples.…”

Section: Discussionmentioning

confidence: 99%

Detection of Escherichia coli O157: H7 from patients with Gastroenteritis

Abdel-Aziz,

Eid

2024

Egyptian Journal of Medical Microbiology

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Background: Acute gastroenteritis is an important public health problem all over the world representing a great economic burden especially in the developing countries. Escherichia coli (E. coli) represents about 30% of bacterial causes especially serotype O157:H7. Infected patients may undergo severe complications in the form of dehydration and hemolytic uremic syndrome (HUS). WHO Global Priority Pathogens (GPP) List reported E. coli O157:H7 as a critical pathogen requiring regular monitoring and surveillance. Objectives: The purpose of this cross sectional study is to highlight the occurrence and determinants factors of this serotype isolated from diarrheal cases in Beni-Suef Governate. Frequent monitoring of foodborne bacteria is essential as they may act as a reservoir carrying and transferring resistance genes to humans. Multidrug resistance (MDR) transmission in this manner would make human bacterial infection is very serious and difficult to be treated. Methodology: The study was carried out on a total 457 cases randomly attended to different hospitals of Beni-Suef Governate (Internal Medicine Outpatient Clinic and Emergency Department) over a period of 2 months. Clinically diagnosed patients with gastroenteritis were included. E. coli O157:H7 was identified by API 20E test kit (bioMérieux, France) and serotyped using latex agglutination test (Oxoid, UK). Sociodemographic factors for cases were also assessed. Results: E. coli O157:H7 was isolated with a prevalence rate 11% (41 out of 457) ; aged from 4-33 years old; (68.3%) males, (51.2%) urban residence and most patients had a habit of outdoor food consumption (70.7%) and a direct contact with animals (82.9%). About 25 cases (61%) needed admission, 10 cases (40%) had moderate dehydration, (13 cases, 52%) had HUS ended with a surviving fate and 2 cases (8%) had HUS ended with death representing the fatality rate. Self-limited gastroenteritis was associated with watery diarrhea with a statistically significant difference; p. value= 0.014. Age among cases who died was lower than survived cases with a significant difference; p. value= 0.006 and all dead patients were children with a statistically significant difference p. value= 0.036. There was no a statistically significant difference regarding sex and residence compared to the fate of admission (p-value> 0.05). Conclusion: E. coli O157:H7 serotype was isolated from Beni-Suef Governate with a relatively high prevalence during the period of the study. Shortage of laboratory facilities to diagnose such pathogen interferes with its definitive diagnosis with subsequent complicated fate for infected cases. Proper handling practices as well as public awareness about the epidemiology of the pathogen should be settled. Vehicles of transmission such as food products and water should be decontaminated to prevent the infection.

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“…The inconvenience of E. coli has been widely reported. Sallam and collaborators [40] highlighted that camel meat may be a vehicle for multi-and extensively drug-resistant E. coli. No Listeria monocytogenes were detected, as Bader and collaborators deduced in dry camel meat [5].…”

Section: Microbiological Changementioning

confidence: 99%

Reformulation of Tunisian Sun-Dried Merguez with Camel Meat: Characterization of Physicochemical and Compositional Changes in Organic Acids, Fatty Acids, Volatile Compounds, and Minerals

Belguith,

Jrad,

Oussaief

et al. 2024

Foods

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Traditional sun-dried merguez is an authentic Tunisian dried sausage made with a large number of spices and herbs, which was reformulated in this study with camel meat and hump fat and dried as in the artisanal process. This research studied the physicochemical, microbiological, and chemical compositional changes that occurred in fresh camel merguez (FCM) after 12 days of drying to achieve traditional dried camel merguez (DCM). The results showed significant weight loss (54.1%), as well as significant decreases in pH (5.20–4.97), moisture (60.5–12.3%), and water activity (0.986–0.673). These results and the acceptable microbiological quality of DCM can explain the safety of traditionally practiced long-term storage at room temperature. All chemical compositions increased upon drying. The composition of DCM included several organic acids, mainly lactate (2820 mg.kg−1); diverse unsaturated fatty acids, in particular oleic acid (33.2%); and various minerals, specifically iron (8 mg per 100 g), in addition to volatile compounds impacted by herbs and spices rich in terpenes (56.3%). These results can be useful for investing in indigenous products and promoting the exploitation of camel meat.

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Cefotaxime-, Ciprofloxacin-, and Extensively Drug-Resistant Escherichia coli O157:H7 and O55:H7 in Camel Meat

Cited by 4 publications

References 49 publications

Prevalence and antibiogram of shigatoxin-producing E. coli in camel meat and offal

Prevalence and antibiogram of shigatoxin-producing E. coli in camel meat and offal

Detection of Escherichia coli O157: H7 from patients with Gastroenteritis

Reformulation of Tunisian Sun-Dried Merguez with Camel Meat: Characterization of Physicochemical and Compositional Changes in Organic Acids, Fatty Acids, Volatile Compounds, and Minerals

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