Ceftiofur Derivatives in Serum, Uterine Tissues, Cotyledons, and Lochia after Fetal Membrane Retention

Abstract

The objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. Concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and lochia during 72 h. After induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12 h were studied. All cows received 3 consecutive injections (C1 to C3; 24 h apart) of 1-mg ceftiofur equivalents per kilogram of body weight as ceftiofur hydrochloride sterile suspension. Samples of blood, endometrium, caruncles, cotyledons, and lochia were collected immediately before each injection (0 h) and again at 4, 12, and 24 h after C1, C2, and C3. Blood samples were collected from coccygeal vessels. Caruncles were removed from the uterine lumen by manual extirpation and separated from cotyledons. Endometrial tissue (0.5 g) was collected by using Kenny's biopsy apparatus. For all samples, concentrations of potentially active ceftiofur derivatives were quantified using an HPLC assay. Within 2 h (serum), 4 h (endometrium), and 12 h (caruncles, cotyledons, lochia) after C1 and during the entire study period, mean concentration of ceftiofur derivatives exceeded the reported minimum drug concentrations required to inhibit the growth of 90% of isolates for relevant bacteria such as Escherichia coli, Fusobacterium necrophorum, and Arcanobacterium pyogenes. Only in single samples did concentrations decrease temporarily below the reported minimum drug concentrations required to inhibit the growth of 90% of isolates.

Assessment of liquid chromatography–tandem mass spectrometry approaches for the analysis of ceftiofur metabolites in poultry muscle

“…Method A resulted in an LOD of 1 mg kg À1 for ceftiofur, 2 mg kg À1 for DFC and 3 mg kg À1 for DCCD. Method B resulted in an LOD of 1 mg kg À1 for the total ceftiofur content, which is superior to previously reported LODs for this approach (10 mg kg À1 for swine muscle by Beconi-Barker et al (1995), 100 mg kg À1 for endometrial tissue by Witte et al (2010) and uterine tissue by Drillich et al (2006)). Method C resulted in an LOD of 0.5 mg kg À1 for the total ceftiofur content, indicating that this method is very suitable for detecting residues of ceftiofur metabolites.…”

“…However, these methods are very laborious, involving several solidphase extraction (SPE) steps. Simplified methods applying the same approach were reported by De Baere et al (2004) for the analysis of plasma and synovial fluid, and by Drillich et al (2006) and Witte et al (2010) for the analysis of several matrices, including serum and endometrial tissue.…”

Efficacy of ampicillin trihydrate or ceftiofur hydrochloride for treatment of metritis and subsequent fertility in dairy cows

“…Administration of ceftiofur at the dose of 1.0 mg/ kg, which is less than the 2.2 mg/kg prescribed dose in United States, resulted in concentrations of ceftiofur derivatives above those capable of inhibiting the growth of utero-pathogenic bacteria (Sheldon et al, 2004;Drillich et al, 2006). The concentration of ampicillin in uterine tissue after systemic administration is undefined, thus it remains unknown if the dose used would lead to optimum to concentrations of ampicillin in the uterine tissues.…”

Inhibitory effects of some drugs on carbonic anhydrase enzyme purified from Kangal Akkaraman sheep in Sivas, Turkey

“…Ceftiofur is a third‐generation line of cephalosporin antibiotics, which is semisynthetic and beta‐lactamase resistant . Ceftiofur also has a very wide range of spectrum.…”

“…[73] Ceftiofur is a third-generation line of cephalosporin antibiotics, which is semisynthetic and beta-lactamase resistant. [74] Ceftiofur also has a very wide range of spectrum. Its main mechanism of activity is by inactivation of penicillin binding proteins (PBPs) located on the inner membrane of bacterial cell walls.…”