Celecoxib inhibits the heterotopic ossification in the rat model with Achilles tenotomy

Abstract

Celecoxib, a selective cox-2 inhibitor, has been shown to prevent the heterotopic ossification following total hip arthroplasty. However, the effects of celecoxib on heterotopic ossification at other locations remain unclear. This study aimed to investigate the effect of celecoxib on heterotopic ossification in the rat model with Achilles tenotomy. Forty male Sprague-Dawley rats, which were randomly divided into 2 groups (n = 20), underwent midpoint Achilles tenotomy on left legs through a posterior approach under aseptic condition. Experimental group was treated with the saline solution of celecoxib (10 mg/kg) per day, while control group was treated by normal saline (0.9%). At 3, 5 and 10 postoperative weeks, all animals were examined by X-ray to assess new bone formation in the Achilles tendon. At 10 weeks after surgery, all animals were killed and Achilles tendons were taken for hematoxylin-eosin (HE) and immunohistochemical staining. Heterotopic ossification developed in 3 rats (15%) in experimental group and 20 rats (100%) in control group by postoperative 10 weeks. The incidence of heterotopic ossification was significantly lower in experimental group than in control group (P < 0.05). Our findings suggest that celecoxib inhibits HO development in rat model with Achilles tenotomy.

Stem cells and bFGF in tendon healing: Effects of lentiviral gene transfer and long-term follow-up in a rat Achilles tendon defect model

“…Ossification occurs as a known process in tendon remodelling [ 3 , 11 ]. Zhang et al [ 21 ] showed in a tenotomy rat achilles tendon model investigating the effect of a selective cox2 inhibitor that heterotopic ossification developed in 100 % of the non treated group after 10 weeks. Similar findings are described by Pietschmann et al [ 22 ] in a tendon defect model with the use of polyglycol acid and collagen-type-I scaffolds, seeded with mesenchymal stem cells or tenocytes.…”

Mechanical loading modulates heterotopic ossification in calcific tendinopathy through the mTORC1 signaling pathway

“…The Achilles tenotomy rat model has been used to investigate HO prophylaxis and treatment based on its relative simplicity and predictability ( 23 – 25 ). In the present study, the Achilles tenotomy rat model was employed to investigate how mechanical loading modulates HO in the tendon.…”

Acute revision hip arthroplasty: a previously unrecognized risk factor for heterotopic ossification

“…The cause may be similar to HO forming following blast injuries or burns. Although the molecular mechanisms of HO have not been fully elucidated in experimental models [35], inflammatory signaling pathways are speculated to play critical roles in HO formation. Forsberg et al [36] have found an association between a patient's systemic and local inflammatory responses and HO.…”