Key words:Rabbits; Venous drainage; Portal vein; gastrointestinal tract.The present work was carried out to investigate the course and distribution of the portal vein, which received all the blood draining from the gastrointestinal tract mainly, in addition to spleen, pancreas and gall bladder. Anatomical studies on the venous drainage of the gastrointestinal tract of the rabbit were necessary to know the pattern of its blood supply to gain information in benefit of experimental surgery, pharmacology and toxicology, which could be applied to domestic animals and human, also helped in the field of veterinary comparative anatomy. Six adult, apparently healthy rabbits of both sexes were carried out in this study the animals were slaughtered, cannulated and flushed with warm normal saline (0.9%). Then animals were injected with blue gum milk latex, the specimens were subjected to fine dissection. The main tributaries of the portal vein were; the gastroduodenal, splenic, left gastric, caudal pancreaticoduodenal and cranial pancreaticoduodenal veins.