Celiac disease‐associated
            <i>Neisseria flavescens</i>
            decreases mitochondrial respiration in CaCo‐2 epithelial cells: Impact of
            <i>Lactobacillus paracasei</i>
            CBA L74 on bacterial‐induced cellular imbalance

Labruna, Giuseppe; Nanayakkara, Merlin; Pagliuca, Chiara; Nunziato, Marcella; Iaffaldano, Laura; D’Argenio, Valeria; Colicchio, Roberta; Budelli, Andrea; Nigro, Roberto; Salvatore, Paola; Barone, Maria Vittoria; Sacchetti, Lucia

doi:10.1111/cmi.13035

Cited by 26 publications

(24 citation statements)

References 41 publications

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“…Furthermore, CD-Nf induced inflammatory phenotypes in ex-vivo mucosal explants, and in both human and murine DCs [25] (Figure 2). We also observed statistically significant lower mitochondrial activity, accompanied by lower ATP production and by higher oxidative stress in CD-Nfinfected Caco-2 cells than in untreated Caco-2 cells [62]. Based on the aforementioned metabolic alterations, we hypothesized that the CD-Nf that we isolated from the duodenum of CD patients, could contribute to the pathogenesis of CD or to disease exacerbation.…”

Section: Host Immune-microbiota Interactionmentioning

confidence: 80%

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Gut microbiome investigation in celiac disease: from methods to its pathogenetic role

Sacchetti

Nardelli

2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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Our body is inhabited by a variety of microbes (microbiota), mainly bacteria, that outnumber our own cells. Until recently, most of what we knew about the human microbiota was based on culture methods, whereas a large part of the microbiota is uncultivable, and consequently previous information was limited. The advent of culture-independent methods and, particularly, of next-generation sequencing (NGS) methodology, marked a turning point in studies of the microbiota in terms of its composition and of the genes encoded by these microbes (microbiome). The microbiome is influenced predominantly by environmental factors that cause a large inter-individual variability (~20%) being its heritability only 1.9%. The gut microbiome plays a relevant role in human physiology, and its alteration (“dysbiosis”) has been linked to a variety of inflammatory gut diseases, including celiac disease (CD). CD is a chronic, immune-mediated disorder that is triggered by both genetic (mainly HLA-DQ2/DQ8 haplotypes) and environmental factors (gluten), but, in recent years, a large body of experimental evidence suggested that the gut microbiome is an additional contributing factor to the pathogenesis of CD. In this review, we summarize the literature that has investigated the gut microbiome associated with CD, the methods and biological samples usually employed in CD microbiome investigations and the putative pathogenetic role of specific microbial alterations in CD. In conclusion, both gluten-microbe and host-microbe interactions drive the gluten-mediated immune response. However, it remains to be established whether the CD-associated dysbiosis is the consequence of the disease, a simple concomitant association or a concurring causative factor.

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Section: Host Immune-microbiota Interactionmentioning

confidence: 80%

“…Numerous studies focused on the putative causal role of specific bacterial groups in the pathogenesis of CD [18,25,27,54,[60][61][62][63][64][65][66]. Below, we summarize results that suggest the putative pathogenic role of specific microbes in CD.…”

Section: The Putative Pathogenic Role Of Cd-associated Dysbiosismentioning

confidence: 99%

Gut microbiome investigation in celiac disease: from methods to its pathogenetic role

Sacchetti

Nardelli

2019

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…The CD-associated Neisseria flavescens was able in inducing inflammation in dendritic cells, in ex-vivo duodenal mucosa explants of healthy controls and in CaCo-2 cells [4]. In the latter cellular model, the CD-associated N. flavescens also induced significant metabolic imbalance by decreasing mitochondrial respiration [5]. Opposite to the dysbiotic profile that we found in a-CD patients, both oral and duodenal microbiome recovered in GFD patients.…”

Section: Resultsmentioning

confidence: 54%

“…The a-CD-associated N. flavescens showed pro-inflammatory activities in vitro, suggesting its potential involvement in the CD-related inflammation [4]. Moreover, we found that the a-CD-associated N. flavescens affected mitochondrial respiration in CaCo-2 epithelial cells [5]. Next, we investigated the oropharyngeal microbiome in CD patients and controls to evaluate whether this niche shared microbial composition with the duodenum [6].…”

Section: Introductionmentioning

confidence: 84%

Setup of Quantitative PCR for Oral Neisseria spp. Evaluation in Celiac Disease Diagnosis

et al. 2019

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Coeliac disease (CD) is a multifactorial autoimmune disorder and gut dysbiosis contributes to its pathogenesis. We previously profiled by 16S rRNA sequencing duodenal and oropharyngeal microbiomes in active CD (a-CD), gluten-free diet (GFD) patients, and controls (CO) and found significantly higher levels of Neisseria spp., with pro-inflammatory activities, in a-CD patients than in the other two groups. In this study, we developed a fast and simple qPCR-based method to evaluate the abundance of the oral Neisseria spp. and the diagnostic performances of the test in CD diagnosis. The Neisseria spp. abundances detected by quantitative PCR (qPCR) were: CO = 0.14, GFD = 0.15, a-CD = 2.08, showing a similar trend to those previously measured by next generation sequencing (NGS). In particular, Neisseria spp. values obtained by both methods were significantly higher (p < 0.001) in a-CD than in the other two groups GFD and CO—the latter almost overlapping. We calculated by ROC curve analysis the threshold of 1.12 ng/μL of Neisseria spp. to discriminate between CO+GFD and a-CD patients with 100% and 96.7% of diagnostic sensitivity and specificity, respectively. In conclusion, our data, if confirmed in other cohorts, suggest the q-PCR evaluation of oral Neisseria spp. could be a fast and simple method to assess CD-associated dysbiosis for diagnostic purposes.

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“…Most of the probiotics on the market are dairy based, although cereals are becoming a viable alternative, as they bypass problems such as lactose intolerance or impact on cholesterolaemia ( Prado et al., 2008 ). Several studies have demonstrated the feasibility of lactic fermentation on cereals and the probiotic effect that fermented cereals can exert ( Sarno et al., 2014 ; Gallo et al., 2018 , 2019a ; Salameh et al., 2019 ; Labruna et al., 2019 ). The advantages offered by cereals therefore represent a boost for the design of new cereals-based foods and ingredients.…”

Section: Introductionmentioning

confidence: 99%

Lactic fermentation of cereals aqueous mixture of oat and rice flours with and without glucose addition

Gallo

Passannanti²,

Cante

et al. 2020

Heliyon

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Studies of the ability of probiotics to ferment cereal flours are necessary to obtain products with enhanced nutritional value. In this study, Lactobacillus paracasei CBA-L74 was used to ferment cereal aqueous mixtures containing both oat (7.5% w/v) and rice flours (7.5% w/v), with and without glucose, to understand whether glucose addition could have any effect on growth and metabolism. Viability, pH, metabolites production during fermentation (24 h, 37 °C) and substrates reduction were analysed. The strain showed good growth in the cereal aqueous mixture both with and without glucose addition, but suspensions prepared with glucose showed the best results. A bacterial concentration of 7 log CFU mL −1 , a pH value of 4.70 and lactic acid production of 1250 mg L −1 were achieved when fermentation was performed without glucose addition, while in the presence of glucose, a t 24 bacterial growth of 8 log CFU mL −1 was reached, with a pH value of 3.11 and lactic acid production of 6050 mg L −1 .

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Celiac disease‐associated Neisseria flavescens decreases mitochondrial respiration in CaCo‐2 epithelial cells: Impact of Lactobacillus paracasei CBA L74 on bacterial‐induced cellular imbalance

Cited by 26 publications

References 41 publications

Gut microbiome investigation in celiac disease: from methods to its pathogenetic role

Gut microbiome investigation in celiac disease: from methods to its pathogenetic role

Setup of Quantitative PCR for Oral Neisseria spp. Evaluation in Celiac Disease Diagnosis

Lactic fermentation of cereals aqueous mixture of oat and rice flours with and without glucose addition

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