Celiac disease: role of intestinal compartments in the mucosal immune response

Iacomino, Giuseppe; Marano, Angela; Stillitano, Ilaria; Aufiero, Vera Rotondi; Iaquinto, Gaetano; Schettino, Maria Teresa; Masucci, Armando; Troncone, Riccardo; Auricchio, Salvatore; Mazzarella, Giuseppe

doi:10.1007/s11010-015-2596-7

Cited by 22 publications

(11 citation statements)

References 30 publications

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“…Rheumatoid arthritis (RA) is a kind of chronic connective tissue immune disease characterized by joint lesions. Its clinical symptoms are manifested as persistent swelling and pain in joints, often involving the interphalangeal joint, elbow joint and pedal joint ( 10 ). The joint swelling and pain in RA patients are severer in the morning, and they are slightly relieved at noon and afternoon.…”

Section: Discussionmentioning

confidence: 99%

Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model

et al. 2018

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The aim of the present study was to investigate the expression levels of interleukin-15 (IL-15) and interleukin-17 (IL-17) in synovial fluid of rheumatoid arthritis (RA) animal model, and to investigate their correlations with RA. A total of 100 Wistar rats were selected, among which 60 rats were used to establish the collagen II-induced arthritis (CIA) model as the model observation group, and the remaining 40 rats were used as blank control group. The levels of IL-15 and IL-17 in synovial fluid were detected via enzyme-linked immunosorbent assay (ELISA) at 1, 7, 14, 21 and 28 days after successful modeling. RA was evaluated by using arthritis index (AI) and pedal swelling volume. The expression levels of IL-15 and IL-17 in synovial fluid of rats in model observation group were higher than those in blank control group (P<0.05), and the levels of IL-15 and IL-17 in model observation group were gradually increased over time. In model observation group at 7 days after modeling, AI and pedal swelling volume began to be increased gradually reaching a peak at 28 days. The pedal swelling volume of CIA model rats was significantly higher than that of the blank control group (P<0.05). The increased expression levels of IL-15 and IL-17 in synovial fluid of rats in the CIA model observation group are correlated with the activity of disease, which can be used as reference indexes for the activity of RA.

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Section: Discussionmentioning

confidence: 99%

Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model

et al. 2018

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“…In this regard, a previous study reported an increase in tumor necrosis factor (TNF)-α-producing innate lymphoid cells in the intestinal mucosa of untreated CD patients in comparison with treated patients and healthy controls[32]. Moreover, an increase in TNF-α and interleukin (IL)-6 levels has been reported in the epithelium and the lamina propia of the intestinal mucosa of untreated CD patients[33,34]. Additionally, higher levels of IL-6 have been observed in the plasma of untreated CD patients compared to treated ones and healthy controls[35,36].…”

Section: Discussionmentioning

confidence: 99%

Markers of inflammation and cardiovascular disease in recently diagnosed celiac disease patients

Tetzlaff¹,

Meroño²,

Menafra³

et al. 2017

WJC

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AIMTo evaluate novel risk factors and biomarkers of cardiovascular disease in celiac disease (CD) patients compared with healthy controls.METHODSTwenty adult patients with recent diagnosis of CD and 20 sex, age and body mass index-matched healthy controls were recruited during a period of 12 mo. Indicators of carbohydrate metabolism, hematological parameters and high sensitive C reactive protein were determined. Moreover, lipoprotein metabolism was also explored through evaluation of the lipid profile and the activity of cholesteryl ester transfer protein and lipoprotein associated phospholipase A2, which is also considered a specific marker of vascular inflammation. The protocol was approved by the Ethic Committee from School of Pharmacy and Biochemistry, University of Buenos Aires and from Buenos Aires Italian Hospital, Buenos Aires, Argentina.RESULTSRegarding the indicators of insulin resistance, CD patients showed higher plasma insulin levels [7.2 (5.0-11.3) mU/L vs 4.6 (2.6-6.7) mU/L, P < 0.05], increased Homeostasis Model Assessment-Insulin Resistance [1.45 (1.04-2.24) vs 1.00 (0.51-1.45), P < 0.05] and lower Quantitative Sensitive Check index [0.33 (0.28-0.40) vs 0.42 (0.34-0.65), P < 0.05] indexes. Folic acid concentration [5.4 (4.4-7.9) ng/mL vs 12.2 (8.0-14.2) ng/mL, P < 0.01] resulted to be lower and High-sensitivity C reactive protein levels higher (4.21 ± 6.47 mg/L vs 0.98 ± 1.13 mg/L, P < 0.01) in the patient group. With respect to the lipoprotein profile, CD patients showed lower high density lipoprotein-cholesterol (HDL-C) (45 ± 15 mg/dL vs 57 ± 17 mg/dL, P < 0.05) and apo A-I (130 ± 31 mg/dL vs 155 ± 29 mg/dL, P < 0.05) levels, as well as higher total cholesterol/HDL-C [4.19 (3.11-5.00) vs 3.52 (2.84-4.08), P < 0.05] and apo B/apo A-I (0.75 ± 0.25 vs 0.55 ± 0.16, P < 0.05) ratios in comparison with control subjects. No statistically significant differences were detected in lipoprotein-associated lipid transfer protein and enzymes.CONCLUSIONThe presence and interaction of the detected alterations in patients with CD, would constitute a risk factor for the development of atherosclerotic cardiovascular disease.

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“…However, the entire small-intestinal cytokine profile of the mice did not resemble that observed in coeliac disease patients (Garrote et al 2008;Lahat et al 1999;Iacomino et al 2016). For instance, no differences in the levels of IL-15, the innate immunity cytokine overexpressed in coeliac patients' small-intestinal epithelia and lamina propria (Di Sabatino et al 2006;Iacomino et al 2016;Maiuri et al 2000;Mention et al 2003),…”

Section: Discussionmentioning

confidence: 91%

“…For instance, no differences in the levels of IL-15, the innate immunity cytokine overexpressed in coeliac patients' small-intestinal epithelia and lamina propria (Di Sabatino et al 2006;Iacomino et al 2016;Maiuri et al 2000;Mention et al 2003),…”

Section: Discussionmentioning

confidence: 99%

Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice

et al. 2016

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Coeliac disease is hallmarked by an abnormal immune reaction against ingested wheat-, rye-and barleyderived gluten and the presence of transglutaminase 2 (TG2)-targeted autoantibodies. The small-bowel mucosal damage characteristic of the disorder develops gradually from normal villus morphology to inflammation and finally to villus atrophy with crypt hyperplasia. Patients with early-stage coeliac disease have TG2-autoantibodies present in serum and small-intestinal mucosa and they may already suffer from abdominal symptoms before the development of villus atrophy. Previously we have shown that intraperitoneal injections of coeliac patient-derived sera or purified immunoglobulin fraction into mice induce a condition mimicking early-stage coeliac disease. In the current study, we sought to establish whether recombinantly produced patient-derived TG2-targeted autoantibodies are by themselves sufficient for the development of such an experimentally induced condition in immune-compromised mice. Interestingly, mice injected with coeliac patient TG2-antibodies had altered small-intestinal mucosal morphology, increased lamina propria cellular infiltration and disease-specific autoantibodies deposited in the small bowel, but did not evince clinical features of the disease. Thus, coeliac patient-derived TG2-specific autoantibodies seem to be sufficient for the induction of subtle small-bowel mucosal alterations in mice, but the development of clinical features probably requires additional factors such as other antibody populations relevant in coeliac disease.

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Celiac disease: role of intestinal compartments in the mucosal immune response

Cited by 22 publications

References 30 publications

Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model

Expression levels of IL-15 and IL-17 in synovial fluid of rheumatoid arthritis animal model

Markers of inflammation and cardiovascular disease in recently diagnosed celiac disease patients

Transglutaminase 2-specific coeliac disease autoantibodies induce morphological changes and signs of inflammation in the small-bowel mucosa of mice

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