Cell adhesion and migration on nanopatterned substrates and their effects on cell-capture yield

Abstract

With scanning electron microscopy analysis, we investigated the role of nanoscale topography on cellular activities; e.g. cell adhesion and spreading by culturing A549 cells (human lung carcinoma cell line cells) for 1-48 h on three sets of nanostructures; quartz nanopillars (QNPs), silicon nanopillars and silicon nanowire (SiNW) arrays, along with planar glass substrates. We found that cells on QNP arrays developed a longer shape than those on SiNW arrays. In addition, we studied how cell morphologies influence the cell-capture yield on the three sets of nanostructures. This research showed that the filopodial formations were directing the cell-capture yield on nanostructured substrates. This finding implies the possibility of using nanoscale topography features to control the filopodial formation including extension and migration from the cells. Using streptavidin-functionalized SiNW substrate, we further demonstrated a substantially higher yield (~91.8 ± 5.9%) than the planar glass wafers (~24.1 ± 7.5%) in the range of 200-3000 cells.

Effects of nanopillar array diameter and spacing on cancer cell capture and cell behaviors

“…Bending compliance affects the adhesion, spreading and migration of attached cells, 32, 35 and was calculated as: $$C=\frac{64h^3}{3\pi {ED}^4}$$ where h and D are the height and diameter of NPs, respectively; E is Young's modulus of NPs with a value of 70 GPa. 36 Fig.…”

“…Captured cells showed either significant lamellipodia or filopodia on nanostructured substrates with certain aspect ratios. 32 …”

Nanowire array chips for molecular typing of rare trafficking leukocytes with application to neurodegenerative pathology

“…Finally, the Ag metal remaining on the Si substrates was completely removed by aqua regia (HCl : HNO 3 = 3 : 1) for 1 h and additional amorphous Si etching for 30 s in buffered oxide etchant (BOE, NH 4 F : HF = 6 : 1). The SiNWs were typically 60 – 100 nm in diameter and 5 – 10 μm in length, which are strongly dependent on the size of Ag nanoparticles and etching time, respectively 82,42 .…”

Enhanced cell growth on nanotextured GaN surface treated by UV illumination and fibronectin adsorption

“…Since UV irradiation can greatly increase both the wettability of GaN surface and the adsorption rate of FN on GaN surface, one can expect that it will provide a favorite environment for cell growth. There are several methods to promote cell adhesion on surfaces [40,41]. Most of them are through chemical modifications to attach the target biomolecules (e.g.…”