Cell-Adhesion Patterning by using Carbon Negative-Ion Implantation into Albumin and Avidin Layers on Polystyrene

Abstract

Carbon negative-ion implantation into proteins of bovine serum albumin (BSA) and avidin to change protein-binding capability for patterning cell adhesion with positive-pattern (PP) and negative-pattern (NP) on polystyrene (PS) were investigated. Hydrophobic PS and hydrophilic one were coated with 5-mg/ml BSA and 500-μg/ml avidin, respectively. After ion implantation at 10 keV and fluences of 1×10 14 -3×10 15 ions/cm 2 , XPS analysis showed decrease of protein amounts on PS with an increase in the ion fluence. Fluorescent dye-conjugated biotin labeling also confirmed the degradation of protein-binding capability. Brightness patterns were found on the implanted region (PP) for C¯-implanted BSA-coated PS (C¯-BSA/PS) with 1-μg/ml avidin coating and on the unimplanted region (NP) for C¯-implantation into avidin-coated PS (C¯-avidin/PS). After culturing nerve-like cell, the clear cell-adhesion patterns of PP and NP were found on the implanted regions for the avidin coated C¯-BSA/PS and on the unimplanted regions for the C¯-avidin/PS at ion fluence of 1×10 14 ions/cm 2 .