Cell age-dependent changes in deformability and calcium accumulation of human erythrocytes

Shiga, Tohru; Sekiya, Misuzu; Maeda, Nobuji; Kon, Kazunori; Okazaki, Masaharu

doi:10.1016/0005-2736(85)90447-x

Cited by 94 publications

(39 citation statements)

References 44 publications

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“…Erythrocytes separated by this method had characteristics of aged cells as demonstrated by measurement of mean corpuscular hemoglobin concentration (MCHC) and mean corpuscular hemoglobin (MCH) from 5 healthy subjects, using the cyanmethemoglobin method (Sigma Chemical Co). We observed a significant (PϽ0.05) and progressive increase in MCHC from the top to the bottom of the cell column (top, 33 …”

Section: Cell Fractionationmentioning

confidence: 83%

“…32 Furthermore, it has been reported that an accumulation of calcium inside the erythrocyte may lead to changes in cytoskeletal conformation and viscoelasticity of the membrane, so the cells would become less deformable. 33,34 Another original finding of our study was the lack of difference in erythrocyte sialic acid content between normotensive and hypertensive subjects, whereas hypercholesterolemia was associated with decreased erythrocyte sialic acid content in both normotensive and hypertensive groups compared with subjects with low cholesterol. The observation of similar levels of membrane sialic acid content in both hypertensive and normotensive groups was in good agreement with the literature data reporting that total sialic acid content in erythrocyte membrane did not differ between normotensive patients and those with essential or renal hypertension.…”

Section: Hadengue Et Al August 1998mentioning

confidence: 91%

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Erythrocyte Disaggregation Shear Stress, Sialic Acid, and Cell Aging in Humans

et al. 1998

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Abstract-Erythrocyte aggregation, which plays an important role in the physiological behavior of blood fluidity, was found to be enhanced in hypertension and hypercholesterolemia. While the role of macromolecule bridging force has been widely described, cellular factors related to membrane sialic acid content, which might contribute to the negative charge of cell surface causing the repulsion of erythrocytes, have been less studied. Cell age-dependent changes in membrane sialic acid content (in micromoles per gram of integral membrane protein) were investigated in 24 normotensive and 24 hypertensive matched subjects, each divided into 2 identical subgroups according to a cutoff of 6.2 mmol/L serum cholesterol. A progressive and significant (PϽ0.001) decrease in membrane sialic acid content associated with an increase (PϽ0.001) of disaggregation shear rate threshold (laser reflectometry in the presence of dextran) were observed with increased erythrocyte density (erythrocytes fractionated by density using ultracentrifugation) in both normotensive and hypertensive groups regardless of the cholesterol level. However, disaggregation shear rate threshold was significantly higher and sialic acid content was lower (PϽ0.001) in both hypertensive and normotensive subjects with hypercholesterolemia compared with either normotensive or hypertensive subjects with low cholesterol, respectively. A high membrane sialic acid content variance, beginning in the younger erythrocytes, was due mainly to triglyceride and LDL cholesterol levels (R 2 ϭ0.49 for low, R 2 ϭ0.43 for middle, and R 2 ϭ0.54 for high densities, ie, young, mean, and senescent erythrocytes, respectively). We conclude that an early decrease in erythrocyte sialic acid content may influence the rheological properties of blood by increasing the adhesive energy of erythrocyte aggregates. (Hypertension. 1998;32:324-330.) Key Words: erythrocyte aggregation Ⅲ rheology Ⅲ sialic acid Ⅲ hypertension, essential Ⅲ hypercholesterolemia E rythrocyte aggregation is one of the main determinants influencing blood circulation at low shear rates by increasing blood viscosity and inducing "sludging" in the capillary.1 Aggregation of red blood cells is a reversible process that occurs when the bridging force due to the adsorption of macromolecules onto adjacent cell surfaces exceeds the disaggregation forces caused by electrostatic repulsion, membrane strain, and mechanical shearing. [1][2][3] An increase in erythrocyte aggregation was found to be associated with cardiovascular risk factors such as hypertension, 4 hyperlipoproteinemia, 5,6 and smoking 7 and in clinical situations such as myocardial ischemia, 8 thromboembolic states, 9 and retinal venous occlusion. 10 In general, most studies have focused on the ability of plasma proteins and various polymers to induce erythrocyte aggregation. We showed that in hypertension and hypercholesterolemia the increase in erythrocyte aggregation could be attributed to an increase in the concentration of plasma fibrinogen. 4,6 In addition, in ...

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Section: Cell Fractionationmentioning

confidence: 83%

Section: Hadengue Et Al August 1998mentioning

confidence: 91%

Erythrocyte Disaggregation Shear Stress, Sialic Acid, and Cell Aging in Humans

et al. 1998

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“…The report by Weed et al [13] was the first to show increased [Ca 2+ ]i to be associated with decreased erythrocyte membrane deformability. Since then, a number of reports have appeared establishing a critical role for maintaining low [Ca 2+ ]i levels in normal erythrocyte mechanical properties [14][15][16]. It has also been reported that high [Ca 2+ ]i values were associated with reduced RBC-df in hypertensive patients [19].…”

Section: Subjectsmentioning

confidence: 99%

Nisoldipine improves the impaired erythrocyte deformability correlating with elevated intracellular free calcium‐ion concentration and poor glycaemic control in NIDDM

Fujita

Tsuda

Takeda

et al. 1999

Brit J Clinical Pharma

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“…Similarly, in a preliminary experiment, the decreased deformability of slightly Ca-loaded erythrocytes was also detected, which could not be observed by the conventional rheoscope (SHIGA et al, 1985;MURAKAMI et al, 1986).…”

mentioning

confidence: 79%

A novel method for measuring the erythrocyte deformability, under oscillatory shear stress.

et al. 1987

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Summary A novel apparatus for detecting a subtle decrease in erythrocyte deformability was developed. The oscillatory shape change of erythrocytes was monitored under an oscillatory shear stress (modulating the oscillation frequency in the range of 9-90 dyn/cm2). The ellipsoidal deformation of intact erythrocytes perfectly followed the oscillatory force of up to about 2 Hz, but the diamide-treated cells showed a phase difference of the oscillatory deformation at 1.7 Hz.Key words erythrocytes, deformability, oscillatory flow.The circulating erythrocytes are repeatedly exposed to the physical stresses which induce cellular deformation. Therefore, it is important to clarify the timedependent process of deformation, with respect to the biochemical properties. Although several quantitative methods have been developed for the measurement of erythrocyte deformability (OKA, 1981), none can be used to observe the oscillatory shape change in the flowing condition.Modifying the "cone-plate rheoscope" (SCHMID-SCHONBEIN and WELLS, 1969;SuDA et al., 1982), we constructed a novel apparatus to apply the oscillatory shear stress to the erythrocyte suspension. The flowing cells exposed to the oscillatory shear stress change their shape alternately, as a function of both the frequency and the amplitude of the oscillatory force. In its sensitivity to a subtle change in deformability, this method is superior to the conventional methods with uniform shear stress.In this note, we describe the design of the apparatus and show a typical example demonstrating its usefulness.Apparatus: As shown in Fig. 1, a transparent 0.8° cone was mounted on an inverted microscope (Olympus Optics, model IMT, Tokyo) and rotated by a stepping motor. The rotation rate of the cone can be modified by modulating the frequency of the driving pulses. The human erythrocytes were immersed in an

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Cell age-dependent changes in deformability and calcium accumulation of human erythrocytes

Cited by 94 publications

References 44 publications

Erythrocyte Disaggregation Shear Stress, Sialic Acid, and Cell Aging in Humans

Erythrocyte Disaggregation Shear Stress, Sialic Acid, and Cell Aging in Humans

Nisoldipine improves the impaired erythrocyte deformability correlating with elevated intracellular free calcium‐ion concentration and poor glycaemic control in NIDDM

A novel method for measuring the erythrocyte deformability, under oscillatory shear stress.

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