Cell Alignment Driven by Mechanically Induced Collagen Fiber Alignment in Collagen/Alginate Coatings

Chaubaroux, Christophe; Perrin-Schmitt, Fabienne; Senger, Bernard; Vidal, Loı̈c; Voegel, Jean‐Claude; Schaaf, Pierre; Haïkel, Youssef; Boulmedais, Fouzia; Lavalle, Philippe; Hemmerlé, Joseph

doi:10.1089/ten.tec.2014.0479

Cited by 46 publications

(42 citation statements)

References 48 publications

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“…Active cell traction forces play a crucial role in the alignment of cells to static uniaxial stretch. Using contact guidance, cells can adjust their orientation to the fibers which align with strain [12,13]. Then, by pulling on the matrix, cells can further align the fibers [14].…”

Section: Introductionmentioning

confidence: 99%

Cell Contractility Facilitates Alignment of Cells and Tissues to Static Uniaxial Stretch

Rens

Merks

2017

Biophysical Journal

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During animal development and homeostasis, the structure of tissues, including muscles, blood vessels, and connective tissues, adapts to mechanical strains in the extracellular matrix (ECM). These strains originate from the differential growth of tissues or forces due to muscle contraction or gravity. Here we show using a computational model that by amplifying local strain cues, active cell contractility can facilitate and accelerate the reorientation of single cells to static strains. At the collective cell level, the model simulations show that active cell contractility can facilitate the formation of strings along the orientation of stretch. The computational model is based on a hybrid cellular Potts and finite-element simulation framework describing a mechanical cell-substrate feedback, where: 1) cells apply forces on the ECM, such that 2) local strains are generated in the ECM and 3) cells preferentially extend protrusions along the strain orientation. In accordance with experimental observations, simulated cells align and form stringlike structures parallel to static uniaxial stretch. Our model simulations predict that the magnitude of the uniaxial stretch and the strength of the contractile forces regulate a gradual transition between stringlike patterns and vascular networklike patterns. Our simulations also suggest that at high population densities, less cell cohesion promotes string formation.

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Section: Introductionmentioning

confidence: 99%

Cell Contractility Facilitates Alignment of Cells and Tissues to Static Uniaxial Stretch

Rens

Merks

2017

Biophysical Journal

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“…Like early efforts developing biomaterials for nerve [3] and cardiac [4] tissue repair, the anisotropy of tendon motivated efforts to develop aligned biomaterials for tendon repair, to increase cell proliferation, enhance the maintenance of a tendon phenotype, and improve extracellular matrix production. Aligned biomaterials, with or without the application of tensile strain, have been shown to provide strong structural cues to direct tenocyte alignment and collagen synthesis, [5] increase MSC proliferation and alignment, [6] and even increased expression levels of tenogenic markers in MSCs and adipose derived stem cells. [7] Similarly, the increased stiffness and mineral content of bone have motivated development of a wide range of mineralized biomaterials with the goal of enhancing MSC osteogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Gradations in Mineral Content, Matrix Alignment, and Applied Strain on Human Mesenchymal Stem Cell Morphology within Collagen Biomaterials

Mozdzen

Thorpe

Screen

et al. 2016

Adv Healthcare Materials

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The tendon-bone junction (TBJ) is a unique, mechanically dynamic, structurally graded anatomical zone which transmits tensile loads between tendon and bone. Current surgical repair techniques rely on mechanical fixation and can result in high re-failure rates. We have recently described a new class of collagen biomaterial that contains discrete mineralized and structurally aligned regions linked by a continuous interface to mimic the graded osteotendinous insertion. Here we report the combined influence of graded biomaterial environment and increasing levels of applied strain (0 – 20%) on MSC orientation and alignment. In osteotendinous scaffolds, which contain opposing gradients of mineral content and structural alignment characteristic of the native osteotendinous interface, MSC nuclear and actin alignment was initially dictated by the local pore architecture, while applied tensile strain enhanced cell alignment in the direction of strain. Comparatively, in layered scaffolds that did not contain any structural alignment cues, MSCs were randomly oriented in the unstrained condition, then became oriented in a direction perpendicular to applied strain. These findings provide an initial understanding of how scaffold architecture can provide significant, potentially competitive, feedback influencing MSC orientation under applied strain, and forms the basis for future tissue engineering efforts to regenerate the osteotendinous enthesis.

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“…[24,26,49] On the other hand, stiffening of the mechanical properties or orientation of the ECM can drive cell phenotype without major changes in the cell integrin ligation profile. [50][51][52] The tensile properties of deposited ECM proteins exposed by our study have potential relevance in experiments focusing on mechanotransduction. For instance, in 2D traction force microscopy (TFM) cell-generated traction forces are inferred from the substrate deformations in combination with the estimated mechanical properties of the used compliant material.…”

Section: Discussionmentioning

confidence: 99%

The Protein Mat(ters) - Revealing Biologically Relevant Mechanical Contribution of Collagen and Fibronectin Coated Micropatterns

Horváth

Holenstein

Silván

et al. 2019

Preprint

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Understanding cell-material interactions requires accurate characterization of substrate mechanics, which are generally measured by indentation-type atomic force microscopy.Although model extracellular matrix coatings are used to facilitate cell-substrate adhesion, their tensile mechanical properties are generally unknown. In this study a novel tensile mechanical characterization of collagen and fibronectin micropatterned polyacrylamide hydrogels is performed. Our findings reveal that the protein coating itself has measurable and biologically relevant consequences, with ligand-specific tensile resistance of the patterned regions relative to the non-patterned surfaces. To our knowledge our study is the first to uncover a direction-dependent mechanical behavior of the protein coatings and to demonstrate that it affects cellular response relative to substrate mechanics.

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Cell Alignment Driven by Mechanically Induced Collagen Fiber Alignment in Collagen/Alginate Coatings

Cited by 46 publications

References 48 publications

Cell Contractility Facilitates Alignment of Cells and Tissues to Static Uniaxial Stretch

Cell Contractility Facilitates Alignment of Cells and Tissues to Static Uniaxial Stretch

The Effect of Gradations in Mineral Content, Matrix Alignment, and Applied Strain on Human Mesenchymal Stem Cell Morphology within Collagen Biomaterials

The Protein Mat(ters) - Revealing Biologically Relevant Mechanical Contribution of Collagen and Fibronectin Coated Micropatterns

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