Cell- and region-specific expression of sugar chains in the mouse epididymal epithelium using lectin histochemistry combined with immunohistochemistry

Abstract

-I B lectin), respectively. These findings indicate that the selectivity in lectin reactivity for distinct cell types and segment-dependent staining in the epididymis may be related to cellular and regional differences in function. Furthermore, because some lectins stain particular cells or cellular compartments selectively, these lectins could be useful markers for histopathological evaluation of diseases or diagnosis of male infertility.

Changes in lectin-binding sites on epididymal cells during postnatal development of the mouse

“…Lectin histochemistry and control staining were performed as described in our previous paper (Tajiri et al, 2012). The 6 biotinylated lectins and their inhibitory sugars used in this study are shown in Table 1.…”

“…The method for double fluorescent staining was identical to the one described in our previous paper (Tajiri et al, 2012).…”

“…We showed that principal cells, clear cells, and basal cells specifically reacted with the lectins UEA-I, MAL-I, and GS-IB, respectively, indicating that these lectins could be useful histochemical markers for basic research in cell differentiation (Tajiri et al, 2012). Therefore, our next approach was to define the behavior of the lectins-binding sites on developing epididymal cells and characterize sugar chain expression in these cells.…”

Histochemical evaluation of postnatal lectin-binding sites in the mouse prostate

“…Their full names (natural sources), abbreviations, carbohydrate specificities, and binding inhibitors are listed in Table 1. Lectin histochemical staining was performed as previously described [26,30]. Briefly, deparaffinized sections were incubated in citrate buffer solution (pH 6.0), and their antigenicities were enhanced in an autoclave (121°C, 1 min).…”

Lectin-binding sites in epithelial cells of the mouse prostate gland

“…Lectin histochemical staining was performed as described in our previous paper 20) . Briefly, deparaffinized sections were incubated with citrate buffer solution (pH 6.0), and their antigenicities were enhanced in an autoclave (121°C, 1 min).…”