Cell and Tissue Imaging with Molecularly Imprinted Polymers as Plastic Antibody Mimics

Kunath, Stephanie; Panagiotopoulou, Maria; Maximilien, Jacqueline; Marchyk, Nataliya; Sänger, Jörg; Haupt, Karsten

doi:10.1002/adhm.201500145

Cited by 119 publications

(88 citation statements)

References 31 publications

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“…Recently, different monosaccharide imprinting procedures were used to produce fluorescently labeled nanoparticle probes displaying an unprecedented affinity for the targeted terminal monosaccharides in cell staining experiments [16]. Based on a ternary complex imprinting approach, we developed imprinted core-shell nanoparticles showing an exceptional affinity for cell surface SA-glycans.…”

Section: Introductionmentioning

confidence: 99%

Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

et al. 2016

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Sialic acid (SA) is normally expressed on the cell membranes and is located at the terminal position of the sugar chains. SA plays an important role for regulation of the innate immunity, function as markers of the cells and can be recognized by a variety of receptors. Interestingly, the level of SA expression is increased on metastatic cancer cells. The availability of specific antibodies against SA is limited and, therefore, biomarker tools for detection of SA are lacking. We have recently presented a novel method for specific fluorescence labeling of SA molecular imprinted polymers (MIP). Here, we have performed an extended screening of SA expression by using SA-MIP and included four different chronic lymphocytic leukemia (CLL) cell lines, conveniently analyzed by flow cytometry and fluorescence microscopy. SA expression was detected in four cell lines at different levels, and the SA expression were verified with lectin-FITC. These results show that SA-MIP can be used as a plastic antibody for detection of SA using both flow cytometry and fluorescence microscopy. We suggest that SA-MIP can be used for screening of different tumor cells of various stages, including CLL cells.

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Section: Introductionmentioning

confidence: 99%

Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

et al. 2016

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“…[11] AM IP shell specific for glucuronic acid (GlcA;g reen-QDs) or N-acetylneuraminic acid (NANA; red-QDs) was grafted on top of the hydrophilic first shell ( Figure 1B)t o target glycosylation sites on cells,s ince altered glycosylation levels or distributions are indicators of infection or malignancy.R ecent advances in glycobiology and cancer research have defined the key processes underlying aberrant glycosylation with sialic acids or hyaluronan in cancer and its consequences. [23][24][25] In this study,M IP-coated QDs were applied for the first time for the simultaneous multiplexed pseudoimmunolabeling and imaging of human keratinocytes.C ore-shell MIP nanoparticles for GlcA and NANA,( 125 AE 17) nm in size, were obtained, thus enabling the specific targeting of both intracellular and pericellular terminal glycosylations.W e previously reported 400 nm rhodamine-labeled MIP particles specific for GlcA that could only target the extracellular hyaluronan of the cell glycocalix. [17,18] MIPs are tailormade synthetic antibody mimics that can recognize and bind target molecules specifically.They are synthesized by copolymerizing functional and cross-linking monomers in the presence of am olecular template,t hus resulting in the formation of binding sites with affinities and specificities comparable to those of natural antibodies.T heir molecularrecognition properties,c ombined with ah igh chemical and physical stability,m ake them interesting substitutes for antibodies in immunoassays, [19] biosensors, [20] bioseparation, [18,21] controlled drug release, [22] and bioimaging.…”

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confidence: 99%

Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

et al. 2016

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Advanced tools for cell imaging are of great interest for the detection, localization, and quantification of molecular biomarkers of cancer or infection. We describe a novel photopolymerization method to coat quantum dots (QDs) with polymer shells, in particular, molecularly imprinted polymers (MIPs), by using the visible light emitted from QDs excited by UV light. Fluorescent core-shell particles specifically recognizing glucuronic acid (GlcA) or N-acetylneuraminic acid (NANA) were prepared. Simultaneous multiplexed labeling of human keratinocytes with green QDs conjugated with MIP-GlcA and red QDs conjugated with MIP-NANA was demonstrated by fluorescence imaging. The specificity of binding was verified with a non-imprinted control polymer and by enzymatic cleavage of the terminal GlcA and NANA moieties. The coating strategy is potentially a generic method for the functionalization of QDs to address a much wider range of biocompatibility and biorecognition issues.

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“…The resultant particles are suitable for applications where antibodies are traditionally used, including sensors22232425 and assays71626. Imprinted nanoparticles have also been shown to selectively detect mammalian cell types by their expression of antigens2728.…”

Section: Resultsmentioning

confidence: 99%

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

Smolińska-Kempisty

Guerreiro

Canfarotta

et al. 2016

Sci Rep

103

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Here we show that molecularly imprinted polymer nanoparticles, prepared in aqueous media by solid phase synthesis with immobilised L-thyroxine, glucosamine, fumonisin B2 or biotin as template, can demonstrate comparable or better performance to commercially produced antibodies in enzyme-linked competitive assays. Imprinted nanoparticles-based assays showed detection limits in the pM range and polymer-coated microplates are stable to storage at room temperature for at least 1 month. No response to analyte was detected in control experiments with nanoparticles imprinted with an unrelated template (trypsin) but prepared with the same polymer composition. The ease of preparation, high affinity of solid-phase synthesised imprinted nanoparticles and the lack of requirement for cold chain logistics make them an attractive alternative to traditional antibodies for use in immunoassays.

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Cell and Tissue Imaging with Molecularly Imprinted Polymers as Plastic Antibody Mimics

Cited by 119 publications

References 31 publications

Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

Different expression levels of glycans on leukemic cells—a novel screening method with molecularly imprinted polymers (MIP) targeting sialic acid

Molecularly Imprinted Polymer Coated Quantum Dots for Multiplexed Cell Targeting and Imaging

A comparison of the performance of molecularly imprinted polymer nanoparticles for small molecule targets and antibodies in the ELISA format

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