Cell-associated amylase activity in Cytophaga johnsonae

McKay, A.M.

doi:10.1111/j.1472-765x.1991.tb00497.x

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…Plasmids much smaller than the size limitation set by the phage head are also efficiently transduced [17]. Plasmid integration into the resident chromosome has been observed after lactose gene transduction and may be caused by IS-mediated transpositional recombination [17][18][19]. The transduction of chromosomal markers is rec dependent -after the erroneous packaging of a fragment of chromosomal DNA in the phage head and transfer it integrates into the proper location in the recipient chromosome by homologous recombination [20].…”

Section: Transductionmentioning

confidence: 99%

Mutations in Lactococcus lactis and their Detection

Kok¹,

Burg²

2006

Genetically Engineered Food

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Many methods are available for permanent alteration or mutation, at will, of the genetic make-up of Lactococcus lactis. Strains with different properties can be selected from natural or industrial environments or can be isolated after application of a variety of classical mutation strategies to existing strains. In the last two decades we have seen the rapid development of sophisticated genetic engineering techniques for application to L. lactis. Recombinant DNA technology has advanced to such perfection that it is now, in principle, possible to introduce any mutation, small or large, or to insert genes from any origin into the genome of L. lactis. These possibilities open up a wide array of new applications of L. lactis, in food or feed production or for entirely new (medical) applications. In this review we describe in detail the potential of altering the genetic make up of L. lactis, by classical techniques and by recombinant DNA technology. We will examine the possibilities of distinguishing the strains made by the latter techniques, so-called genetically modified organisms, from "natural" mutants and will discuss methods for detection of genetically modified strains of L. lactis. IntroductionGenetically modified organisms (GMOs) are organisms in which permanent DNA alterations have been introduced by recombinant DNA techniques. Over the last two decades, Lactococcus lactis has been made amenable to recombinant DNA technology. Tools to transform L. lactis, to introduce and express (foreign) DNA, to secrete (heterologous) proteins, and to mutate the chromosome by single and double crossover recombination strategies have all been developed. Constitutive or regulated promoters can be used to drive (foreign) gene expression. The culmination of genetic dissection of L. lactis was the determination in 2001 of the entire nucleotide sequence of the chromosome of L. lactis subsp. Lactis IL1403 [1a].

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Section: Transductionmentioning

confidence: 99%

Mutations in Lactococcus lactis and their Detection

Kok¹,

Burg²

2006

Genetically Engineered Food

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“…To allow both a better genetic analysis and strain improvement, there is a need for DNA transfer systems in these microorganisms. Conjugative transfer of plasmids [1][2][3] and transduction [4] have been reported in lactococci. PEGinduced-protoplast-transformation has also been described [5,6] but this method has several serious drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Effect of restriction-modification systems on transfer of foreign DNA into Lactococcus lactis subsp. lactis

Langella

1989

FEMS Microbiology Letters

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Cell-associated amylase activity in Cytophaga johnsonae

Cited by 2 publications

References 10 publications

Mutations in Lactococcus lactis and their Detection

Mutations in Lactococcus lactis and their Detection

Effect of restriction-modification systems on transfer of foreign DNA into Lactococcus lactis subsp. lactis

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