Cell-based bioreporter assay coupled to HPLC micro-fractionation in the evaluation of antimicrobial properties of the basidiomycete fungus <i>Pycnoporus cinnabarinus</i>

Järvinen, Päivi; Nybond, Susanna; Marcourt, Laurence; Queiroz, Emerson Ferreira; Wolfender, Jean‐Luc; Mettälä, Aila; Karp, Matti; Vuorela, Heikki; Vuorela, Pia; Hatakka, Annele; Tammela, Päivi

doi:10.3109/13880209.2015.1103754

Cited by 10 publications

(2 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Such analyses point out compounds with the desired activity in a complex matrix, irrespectively of their chemical nature and ensure a subsequent highly targeted procedure including characterization, bioactivity assayguided isolation and identification of the components in question [1][2][3]. Planar layer chromatography coupled with antibacterial assay (direct bioautography, DB) [4,5] is a screening and biomonitoring tool that is high-throughput, rapid, easy to perform and suitable in such a workflow [6,7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Layer chromatography-bioassays directed screening and identification of antibacterial compounds from Scotch thistle

Móricz

Krüzselyi

Alberti

et al. 2017

Journal of Chromatography A

View full text Add to dashboard Cite

Highlights:-First direct transfer of OPLC mobile phase to flash chromatography -Antibacterial profiling of Scotch thistle (Onopordum acanthium L.) leaf extract -TLC/OPLC-DB using plant and human pathogen bacteria and a probiotic, human gut bacterium L. plantarum utilized for investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria. Antibacterial fractions obtaining infusion-transfusion OPLC were transferred to HPLC-MS/MS analysis that resulted in the characterization of three active compounds and two of them were identified as, linoleic and linolenic acid. OPLC method was adopted to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Planar layer chromatography coupled with antibacterial assay (direct bioautography, DB) [4,5] is a screening and biomonitoring tool that is high-throughput, rapid, easy to perform and suitable in such a workflow [6,7]. Compared to HPLC-antibacterial assay [1,2], the benefits of DB are: 1.…”

Section: Introductionmentioning

confidence: 99%