Cell-Based Biosensors and Their Application in Biomedicine

Liu, Qingjun; Wu, Chunsheng; Hua, Cunqing; Hu, Ning; Zhou, Jun; Wang, Ping

doi:10.1021/cr2003129

Cited by 321 publications

(215 citation statements)

References 423 publications

Supporting

Mentioning

214

Contrasting

Unclassified

Order By: Relevance

“…Also, analytical biosensors can be integrated directly into the culture platform, thus combining living cells and sensors for detection of cellular physiological parameters and analysis of external stimuli in situ 5 .…”

Section: Introductionmentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

View full text Add to dashboard Cite

A compact microfludic device for perfusion culture of mammalian cells under in-situ metabolomic observation by NMR spectroscopy is presented. The chip is made from poly(methyl methacrylate) (PMMA), and uses a poly(dimethyl siloxane) (PDMS) membrane to allow gas exchange. It is integrated with a generic micro-NMR detector developed recently in our group [J. Magn. Reson. 262, 73-80 (2016)]. While PMMA is an excellent material in the context of NMR, PDMS is known to produce strong background signals. To mitigate this, the device keeps the PDMS away from the detection area. The oxygen permeation into the device is quantified using a flow chemistry approach. A solution of glucose is mixed on the chip with one of glucose oxidase, before flowing through the gas exchanger. The resulting concentration of gluconate is measured by 1 H NMR spectroscopy as a function of flow rate. An oxygen equilibration rate constant of 2.4 s −1 is found for the device, easily sufficient to maintain normoxic conditions in a cell culture at modest perfusion flow rates.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Yılmaz

Utz

2016

Lab Chip

View full text Add to dashboard Cite

show abstract

“…Electrochemical imaging methods are alternative techniques for live-cell imaging, including scanning electrochemical microscopy (SECM) [4,5], scanning ion conductance microscopy (SICM) [6,7], plasmonic-based electrochemical impedance microscopy (EIM) [8], and lightaddressable potentiometric sensors (LAPS) [9]. They can map parameters such as Faradaic current, ion conductivity, local impedance, extracellular potentials and charges, which cannot be measured with optical microscopy.…”

Section: Introductionmentioning

confidence: 99%

Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

Zhang

Wang

et al. 2016

Electrochemistry Communications

View full text Add to dashboard Cite

“…6,25,[29][30][31] Although bioelectronic noses have good selectivity and sensitivity, they still suffer from intrinsic and external noise interference.…”

Section: Discussionmentioning

confidence: 99%

“…For biosensor applications, MEA coupled with biological elements in vitro could be considered as a more advanced and high sensitive system, which strongly depends on the compatibility between its electrodes and the bio-recognition elements. [23][24][25] As a result, it was a valuable tool for long-time network-level electrophysiological investigations, but also persecuted by noises. Here, a NanoZn-equipped biosensor, based on olfactory receptor cells combined with MEA, was further developed in order to explore analytical methods for improving the performance of olfactory biosensors.…”

Section: Introductionmentioning

confidence: 99%

Zinc Nanoparticles-equipped Bioelectronic Nose Using a Microelectrode Array for Odorant Detection

Zhang

et al. 2016

ANAL. SCI.

Self Cite

View full text Add to dashboard Cite

Bioelectronic noses, such as olfactory cell-and receptor-based biosensors, have important applications for biomimetic odorant detection in various fields. Here, a nanoparticle-equipped biosensor was designed to record extracellular potentials from olfactory receptor cells effectively. In this research, a microelectrode array (MEA) was combined with olfactory epitheliums as the olfactory biosensor to record electrophysiological signals of receptor cells in the epitheliums. Zinc nanoparticles (NanoZn) were employed along with the biosensor for different kinds of odorant measurements, which improved the electrophysiological responses to odor molecules. The NanoZn-equipped biosensor showed greater performance, such as a higher sensitivity and a larger signal-to-noise ratio, than that without the nanoparticles. Thus, this approach provided a promising method to improve the detecting performance of biosensors based on olfactory cells and receptors, which would bring broad application prospects for bioelectronic noses in environmental monitoring, food analysis, and healthcare diagnosis.

show abstract

Cell-Based Biosensors and Their Application in Biomedicine

Abstract: Figure 17. Illustration of an LAPS as a microphysiometer: (a, b) principle and structure of the LAPS, (c) proton release in cellular metabolism. Panel a reprinted with permission from ref 309.

Cited by 321 publications

References 423 publications

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Characterisation of oxygen permeation into a microfluidic device for cell culture by in situ NMR spectroscopy

Image detection of yeast Saccharomyces cerevisiae by light-addressable potentiometric sensors (LAPS)

Zinc Nanoparticles-equipped Bioelectronic Nose Using a Microelectrode Array for Odorant Detection

Contact Info

Product

Resources

About