Cell-Based Biosensors: Electrical Sensing in Microfluidic Devices

Kiilerich-Pedersen, Katrine; Rozlosnik, Noémi

doi:10.3390/diagnostics2040083

Cited by 25 publications

(13 citation statements)

References 67 publications

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“…But they lack specificity because of presence of unwanted enzymes which leads to ambiguous catalytic reactions [ 15 , 33 ]. Single-cell analysis of neuronal cells during neuronal regeneration can be achieved by quantitative measurement of cellular transmitter released by the cells trapped in a closed microchip close to a band of microelectrodes [ 34 ]. Cell based microfluidic technology is most suitable for cell migration assay and invasion assay applicable in drug screening.…”

Section: Biosensing Elementsmentioning

confidence: 99%

Biosensors in Health Care: The Milestones Achieved in Their Development towards Lab-on-Chip-Analysis

Patel

Nanda

Sahoo

et al. 2016

Biochemistry Research International

142

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Immense potentiality of biosensors in medical diagnostics has driven scientists in evolution of biosensor technologies and innovating newer tools in time. The cornerstone of the popularity of biosensors in sensing wide range of biomolecules in medical diagnostics is due to their simplicity in operation, higher sensitivity, ability to perform multiplex analysis, and capability to be integrated with different function by the same chip. There remains a huge challenge to meet the demands of performance and yield to its simplicity and affordability. Ultimate goal stands for providing point-of-care testing facility to the remote areas worldwide, particularly the developing countries. It entails continuous development in technology towards multiplexing ability, fabrication, and miniaturization of biosensor devices so that they can provide lab-on-chip-analysis systems to the community.

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Section: Biosensing Elementsmentioning

confidence: 99%

Biosensors in Health Care: The Milestones Achieved in Their Development towards Lab-on-Chip-Analysis

Patel

Nanda

Sahoo

et al. 2016

Biochemistry Research International

142

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“…The combination of droplet microfluidics with electrosensing techniques such as electrochemical impedance spectroscopy (EIS), field-effect measurements, waveguide-based techniques, amperometry, cyclic voltammetry, etc. would allow obtaining different information to be applied in a broad range of applications [34][35][36][37][38][39]. However, the implementation of nanostructures on the sensing areas exploiting their high sensitivity is expected to further enhance the power of these miniaturized sensors.…”

Section: Introductionmentioning

confidence: 99%

Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets

et al. 2020

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Here, we present a miniaturized lab-on-a-chip detecting system for an all-electric and label-free analysis of the emulsion droplets incorporating the nanoscopic silicon nanowires-based field-effect transistors (FETs). We specifically focus on the analysis of β-galactosidase e.g., activity, which is an important enzyme of the glycolysis metabolic pathway. Furthermore, the efficiency of the synthesis and action of β-galactosidase can be one of the markers for several diseases, e.g., cancer, hyper/hypoglycemia, cell senescence, or other disruptions in cell functioning. We measure the reaction and reaction kinetics-associated shift of the source-to-drain current Isd in the system, which is caused by the change of the ionic strength of the microenvironment. With these results, we demonstrate that the ion-sensitive FETs are able to sense the interior of the aqueous reactors; thus, the conjunction of miniature nanosensors and droplet-based microfluidic systems conceptually opens a new route toward a sensitive, optics-less analysis of biochemical processes.

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“…These new possibilities are viable due to invested efforts in development and integration of sensor, actuation and microfluidics technologies. Sensing technologies in the microfluidic environment are numerous [2,3,4,5,6,7,8,9,10] and most often present optical [7,8] or electrical [3,4] signal at the output of a measurement transducer. In particular, every sensing technology has its advantages and disadvantages, with respect to price, size, integration capability, multi-functionality, need for labels, fabrication methods, and many other [10,11].…”

Section: Introductionmentioning

confidence: 99%

An Interdigital Capacitor for Microwave Heating at 25 GHz and Wideband Dielectric Sensing of nL Volumes in Continuous Microfluidics

Markovic

Bao

Maenhout

et al. 2019

Sensors

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This paper proposes a miniature microwave-microfluidic chip based on continuous microfluidics and a miniature interdigital capacitor (IDC). The novel chip consists of three individually accessible heaters, three platinum temperature sensors and two liquid cooling and mixing zones. The IDC is designed to achieve localized, fast and uniform heating of nanoliter volumes flowing through the microfluidic channel. The heating performance of the IDC located on the novel chip was evaluated using a fluorescent dye (Rhodamine B) diluted in demineralized water on a novel microwave-optical-fluidic (MOF) measurement setup. The MOF setup allows simultaneous microwave excitation of the IDC by means of a custom-made printed circuit board (connected to microwave equipment) placed in a top stage of a microscope, manipulation of liquid flowing through the channel located over the IDC with a pump and optical inspection of the same liquid flowing over the IDC using a fast camera, a light source and the microscope. The designed IDC brings a liquid volume of around 1.2 nL from room temperature to 100 °C in 21 ms with 1.58 W at 25 GHz. Next to the heating capability, the designed IDC can dielectrically sense the flowing liquid. Liquid sensing was evaluated on different concentration of water-isopropanol mixtures, and a reflection coefficient magnitude change of 6 dB was recorded around 8.1 GHz, while the minimum of the reflection coefficient magnitude shifted in the same frequency range for 60 MHz.

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Cell-Based Biosensors: Electrical Sensing in Microfluidic Devices

Cited by 25 publications

References 67 publications

Biosensors in Health Care: The Milestones Achieved in Their Development towards Lab-on-Chip-Analysis

Biosensors in Health Care: The Milestones Achieved in Their Development towards Lab-on-Chip-Analysis

Nanosensors-Assisted Quantitative Analysis of Biochemical Processes in Droplets

An Interdigital Capacitor for Microwave Heating at 25 GHz and Wideband Dielectric Sensing of nL Volumes in Continuous Microfluidics

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