Cell-Based Chemical Safety Assessment and Therapeutic Discovery Using Array-Based Sensors

Abstract

Synthetic chemicals are widely used in food, agriculture, and medicine, making chemical safety assessments necessary for environmental exposure. In addition, the rapid determination of chemical drug efficacy and safety is a key step in therapeutic discoveries. Cell-based screening methods are non-invasive as compared with animal studies. Cellular phenotypic changes can also provide more sensitive indicators of chemical effects than conventional cell viability. Array-based cell sensors can be engineered to maximize sensitivity to changes in cell phenotypes, lowering the threshold for detecting cellular responses under external stimuli. Overall, array-based sensing can provide a robust strategy for both cell-based chemical risk assessments and therapeutics discovery.

Direct discrimination of cell surface glycosylation signatures using a single pH-responsive boronic acid-functionalized polymer

“…23 Array-based sensing can provide a highly sensitive sensing platform to discriminate complicated bio-analytes, 24 including proteins, [25][26][27] bacteria, [28][29][30] and cell phenotypes, [31][32][33] using selective and differential interaction between sensor elements and target analytes. 34 Polymeric sensor array platforms have emerged as promising strategies due to their high stability, reproducibility, and scalability. 35,36 Previously, our lab fabricated a three-channel sensor using gold nanoparticles featuring arginine ligand conjugated with uorescent proteins to identify mammalian cells based on cell-surface glycosylation pattern.…”

Nanozyme Inhibited Sensor Array for Biothiol Detection and Disease Discrimination Based on Metal Ion-Doped Carbon Dots

“…By mimicking mammals’ gustatory or olfactory systems, array-based sensing methods are powerful approaches for the simultaneous determination and discrimination of multiple analytes with similar structure or chemical characteristics. − The sensor array is composed of cross-responsive sensing elements, which is different from ‘‘key-and-lock” sensors consisting of specific receptors, to produce composite responses for detection and identification of various targets. − Many kinds of quantum dot based sensor arrays have been devised for the identification and detection of biothiols. − However, complicated synthesis and low sensitivity limit their practical applicability. Hence, the further development of highly sensitive and cost-effective sensor arrays for biothiol monitoring is still desirable.…”

Identification of Proteins Using Supramolecular Gold Nanoparticle‐Dye Sensor Arrays

“…Cross‐reactive sensor arrays enable the high‐throughput detection of multiple analytes by differential interactions between sensor elements and analytes [9–11] . Sensor arrays have emerged as an efficient tool for detecting bioanalytes, including proteins, [12,13] bacteria [14,15] and cells [16,17] .…”

“…Cross-reactive sensor arrays enable the high-throughput detection of multiple analytes by differential interactions between sensor elements and analytes. [9][10][11] Sensor arrays have emerged as an efficient tool for detecting bioanalytes, including proteins, [12,13] bacteria [14,15] and cells. [16,17] Sensor arrays can be fabricated using a variety of materials, [18] such as gold nanoparticles (AuNPs), [19] polymers [20] and small molecules.…”