Cell-based meat: The molecular aspect

Abstract

Cellular agriculture is one of the evolving fields of translational biotechnology. The emerging science aims to improve the issues related to sustainable food products and food security, reduce greenhouse gas emissions and provide animal wellbeing by circumventing livestock farming through cell-based meat (CBM) production. CBM exploits cell culture techniques and biomanufacturing methods by manipulating mammalian, avian, and fish cell lines. The cell-based products ought to successfully meet the demand for nutritional protein products for human consumption and pet animals. However, substantial advancement and modification are required for manufacturing CBM and related products in terms of cost, palatability, consumer acceptance, and safety. In order to achieve high-quality CBM and its production with high yield, the molecular aspect needs a thorough inspection to achieve good laboratory practices for commercial production. The current review discusses various aspects of molecular biology involved in establishing cell lines, myogenesis, regulation, scaffold, and bioreactor-related approaches to achieve the target of CBM.

Sistema Cardiovascular Bovino: Conservação E Montagem

“…2021). Cell-based meat represents a promising strategy that could offer tools for nutritional enrichment and sustainable seafood generation without the environmental impact associated with traditional methods (Azhar et al 2023).…”

Responsible innovation: Mitigating the food safety aspects of cultured meat production

“…Some food production interventions have been developed to prevent telomere shortening in germ cells and reduce the risk of cell senescence that can lead to the overexpression of a catalytically active subunit of telomerase (Soice & Johnston, 2021). Patents have been submitted to use telomerase reverse transcriptase (TERT) via CRISPR technology to knock out some gene expression (p16) and cause overexpression of TERT (Azhar et al, 2023;Thorley et al, 2016). Thus, using either spontaneously immortalized or modified cell lines may have ethical and probity-related concerns for consumers and regulators.…”

“…Glucose is another essential base nutrient as well as minor materials such as vitamins, insulin, transferrin, fibroblast growth factor (FGF) and transforming growth factor (TGF), salts, minerals, and buffers such as 4-(2hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Risner et al, 2023). Metabolites produced during the cultured meat process that could inhibit cell growth include lactate produced from the conversion of glucose (Azhar et al, 2023;Risner et al, 2023) and ammonia (Risner et al, 2023). FBS is a biochemically suitable growth medium as it contains adhesion molecules, carbohydrates, cytokines, essential amino acids, growth factors, hormones, nonprotein nitrogen, serum protein, trace elements, and vitamins (Lee et al, 2022;Pilgrim et al, 2022), but it has had cells, platelets, and clotting factors removed (Risner et al, 2023).…”