Cell-Based Model Systems for Validation of Various Efficacy-Based Claims for Cosmetic Ingredients

Abstract

Cosmetic formulations have evolved significantly over the years. These are no longer viewed merely as beauty-enhancing products. Rather, they are expected to deliver additional benefits to the skin that positively affect the skin health. There is a renewed interest in using herbal extracts and herbal ingredients in cosmetic products since they offer several advantages over synthetic ingredients. Evaluating the cosmetic ingredients for their efficacy and safety is critical during product development. Several regulatory bodies impose restrictions on using animals for testing these ingredients in cosmetic products. This has increased the need for developing novel cell-based or cell-free biological assays. The current article systematically presents in-vitro/cell-based and/or cell-free strategies for validating the efficacies of cosmetic ingredients for skin health and hair growth. The article focuses on details about various assays for the anti-acne effects, hair-growth-promoting activities, anti-aging activities, skin-rejuvenating properties, wound-healing effects, and skin-depigmentation activities of natural ingredients in cosmetic formulations.

Microspheres Based on Blends of Chitosan Derivatives with Carrageenan as Vitamin Carriers in Cosmeceuticals

“…Additionally, the in vitro assays for the safety evaluation of cosmetic ingredients and products respect the 3R's principles of replacing animal use [55]. Cell-based testing systems have a few advantages over other methods for the validation of efficacy because they are cost-effective, adaptable for high throughputs, and yield results considerably faster than conventional in vivo methods [56]. Multiple mechanisms and cell types are involved in the induction of skin toxicological responses, e.g., skin irritation involves resident epidermal cells, dermal fibroblasts, and endothelial cells, as well as invading leukocytes that interact with each other under the control of a network of cytokines and lipid mediators [57].…”

Developing Botanical Formulations for Sustainable Cosmetics

“…Cosmetics research and the well-being market are moving towards natural cosmetics and botanic formulations in order to obtain more effective and dermo-safe products according to the demands for sustainability [18].…”

Skin-Whitening Effect of a Callus Extract of Nelumbo nucifera Isolate Haman

“…In general, the creams used for the clinical tests had a higher concentration of the target material than the concentration confirmed by the in vitro tests. This is generally due to official policies and numerous legislations in the cosmetics industry, as well as the outcomes of research [62,63]. The main reason for the higher dose was the rate of skin penetration of the targeted materials included in the products.…”