Cell-Based Screening Test for Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Akoume, Marie-Yvonne; Azeddine, Bouziane; Turgeon, Isabelle; Franco, Anita; Poitras, Benoît; Rivard, Charles‐Hilaire; Grimard, Guy; Ouellet, Jean; Parent, Stefan; Moreau, Alain

doi:10.1097/brs.0b013e3181cf39ff

Cited by 30 publications

(34 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…As expected, all patients were less responsive than control subjects, and patients classified in the same functional group by our new procedure exhibited similar levels of the maximum response ( Figure 5). Moreover, disparity between patients of each functional group was consistent with our classical range of classification 12 , validating our new procedure. The classification of a large cohort of scoliotic patients regularly followed in our special clinic at Sainte-Justine Hospital has revealed that the three functional groups were similarly distributed among moderate cases, while the FG2 was predominant among severe cases (Figure 6), identifying patients categorized into this functional group as more at risk for severe progression of the disease and indicating that this classification test can be useful in the prognosis of idiopathic scoliosis.…”

Section: Protocolsupporting

confidence: 84%

“…Using this procedure, asymptomatic and scoliotic children can be easily classified to better predict their clinical outcome without any danger for their health. However, when performing classification according to the degree of maximum response to Gi stimulation relative to the healthy control subjects 12 , several requirements for the control subjects should be met. Indeed, in order to have a proper comparison cohort, the control subjects must be age and gender matched, not be on any kind of medication, and provide private information, such as past individual/familial medical history.…”

Section: Discussionmentioning

confidence: 99%

“…With this approach, the scoliotic patients and children more at risk of developing scoliosis are less responsive to Gi protein stimulation when compared with healthy control subjects, and the classification is based on the percentage of degree of reduction relative to control group. The classification ranges are fixed between 10 and 40% for FG3, 40 and 60% for FG2, and 60 and 90% for FG1 12 .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Akoume¹,

Franco²,

Moreau

2013

JoVE

Self Cite

View full text Add to dashboard Cite

This protocol details the experimental and analytical procedure for a cell-based assay developed in our laboratory as a functional test to predict the prognosis of idiopathic scoliosis in asymptomatic and affected children. The assay consists of the evaluation of the functional status of Gi and Gs proteins in peripheral blood mononuclear cells (PBMCs) by cellular dielectric spectroscopy (CDS), using an automated CDS-based instrument, and the classification of children into three functional groups (FG1, FG2, FG3) with respect to the profile of imbalance between the degree of response to Gi and Gs proteins stimulation. The classification is further confirmed by the differential effect of osteopontin (OPN) on response to Gi stimulation among groups and the severe progression of disease is referenced by FG2. Approximately, a volume of 10 ml of blood is required to extract PBMCs by Ficoll-gradient and cells are then stored in liquid nitrogen. The adequate number of PBMCs to perform the assay is obtained after two days of cell culture. Essentially, cells are first incubated with phytohemmaglutinin (PHA). After 24 hr incubation, medium is replaced by a PHA-free culture medium for an additional 24 hr prior to cell seeding and OPN treatment. Cells are then spectroscopically screened for their responses to somatostatin and isoproterenol, which respectively activate Gi and Gs proteins through their cognate receptors. Both somatostatin and isoproterenol are simultaneously injected with an integrated fluidics system and the cells' responses are monitored for 15 min. The assay can be performed with fresh or frozen PBMCs and the procedure is completed within 4 days.

show abstract

Section: Protocolsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Akoume¹,

Franco²,

Moreau

2013

JoVE

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many of these receptors mediate the transmission of intracellular signals by activating guanine nucleotide-binding proteins (G proteins) to which the receptor is coupled. Such receptors are generically referred to as G protein-coupled receptors ("GPCR"s) [30]. Ghrelin secretion is stimulated by several Gs-coupled receptors and inhibited by many Gicoupled and Gq receptors [31].…”

Section: Discussionmentioning

confidence: 99%

Fasting total ghrelin levels are increased in patients with adolescent idiopathic scoliosis

Gennero¹,

Conte-Auriol²,

Mus³

et al. 2013

View full text Add to dashboard Cite

Background: A control study was designed to investigate circulating Ghrelin levels in adolescent girls with adolescent idiopathic scoliosis (AIS) and controls. Eating behavioral disorders, endocrine disorders, abnormal growth pattern and osteopenia have been well documented in AIS. Ghrelin is an orexigenic hormone produced by the stomach which reflects body weight changes and stimulates growth hormone secretion. Recently, it has been shown to be associated with bone metabolism and eating behavior. However, the circulating levels of ghrelin have never been evaluated in AIS patients. Methods: Forty nine AIS girls and 15 controls were included. Anthropometric parameters and fasting circulating total ghrelin were measured. Curve severity was evaluated in AIS girls. The relationships between ghrelin and age, body weight, height, body mass index (BMI), BMI Z-score and corrected anthropometric parameters were analyzed in AIS girls and controls. Results: There was no significant difference in body weight, height, BMI or BMI Z-score between AIS and controls. Serum ghrelin level was 1.8 fold higher in AIS girls than in controls. Elevation of ghrelin levels remained significant when corrected BMI or corrected BMI Z-score were considered. Unlike in controls, positive correlations were found between ghrelin and age in AIS girls with a gradual increase of circulating ghrelin with age. Conclusions: We have observed significantly higher circulating ghrelin levels in AIS than in controls with a positive correlation with age. This pilot-study suggests that ghrelin signaling might play a role in the initiation or development of AIS. Further studies are needed to validate theses results.

show abstract

“…Such techniques have been applied to patients with AIS but, unfortunately, the picture that has emerged does not seem to be a simple one. Genetic research into AIS has shown many genes possibly to be involved and the picture is most certainly complex even though diagnostic tests are currently being developed (Akoume et al, 2010;Leboeuf et al, 2009;Ogilvie, 2010;Weinstein et al, 2008). This complexity might well reflect the complex nature of AIS (as described above) and the mixed nature of patients with possibly very different underlying causes.…”

Section: The Promise Of Genetic Studiesmentioning

confidence: 99%

How to Improve Progress in Scoliosis Research

Bagnall¹

2012

Recent Advances in Scoliosis

View full text Add to dashboard Cite

Cell-Based Screening Test for Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Cited by 30 publications

References 23 publications

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Cell-based Assay Protocol for the Prognostic Prediction of Idiopathic Scoliosis Using Cellular Dielectric Spectroscopy

Fasting total ghrelin levels are increased in patients with adolescent idiopathic scoliosis

How to Improve Progress in Scoliosis Research

Contact Info

Product

Resources

About