Cell-Based Systems of Depression: An Overview

Jantas, Danuta

doi:10.1007/978-3-319-14021-6_3

Cited by 6 publications

(6 citation statements)

References 268 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, drugs that increase the expression of HIF-1 may be a promise in the therapeutic treatment of depression [ 13 , 14 , 15 ]. In this study, we aimed to explore the connection between hypoxic environments and the effect of non-cytotoxic ( Figure 5 ) low (10 nM) and high (20 µM) concentrations of Mirtazapine [ 23 ] and other drugs with the potential to be repurposed as antidepressant drugs in SH-SY5Y human neuroblastoma cells, a cell line widely used in the research of neuropsychiatric and neurological disorders and mechanisms [ 28 ]. In fact, multiple drugs that were originally developed for different medical conditions have demonstrated promise in the treatment of depression.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Role of Drug Repurposing in Bridging the Hypoxia–Depression Connection

Correia,

Marques,

Cardoso

et al. 2023

Membranes

View full text Add to dashboard Cite

High levels of oxidative stress are implicated in hypoxia, a physiological response to low levels of oxygen. Evidence supports a connection between this response and depression. Previous studies indicate that tryptophan hydroxylase can be negatively affected in hypoxia, impairing serotonin synthesis and downstream pathways. Some studies also hypothesize that increasing hypoxia-inducible factor-1 (HIF-1) levels may be a new therapeutic modality for depression. Hence, this study delved into the influence of hypoxia on the cellular response to drugs designed to act in depression. By the induction of hypoxia in SH-SY5Y cells through a hypoxia incubator chamber or Cobalt Chloride treatment, the effect of Mirtazapine, an antidepressant, and other drugs that interact with serotonin receptors (TCB-2, Dextromethorphan, Ketamine, Quetiapine, Scopolamine, Celecoxib, and Lamotrigine) on SH-SY5Y cellular viability and morphology was explored. The selection of drugs was initially conducted by literature search, focusing on compounds with established potential for employment in depression therapy. Subsequently, we employed in silico approaches to forecast their ability to traverse the blood–brain barrier (BBB). This step was particularly pertinent as we aimed to assess their viability for inducing potential antidepressant effects. The effect of these drugs in hypoxia under the inhibition of HIF-1 by Echinomycin was also tested. Our results revealed that all the potential repurposed drugs promoted cell viability, especially when hypoxia was chemically induced. When combined with Echinomycin, all drugs decreased cellular viability, possibly by the inability to interact with HIF-1.

show abstract

Section: Discussionmentioning

confidence: 99%

Exploring the Role of Drug Repurposing in Bridging the Hypoxia–Depression Connection

Correia,

Marques,

Cardoso

et al. 2023

Membranes

View full text Add to dashboard Cite

show abstract

“…Therefore, understanding the connection between MDD and DM requires an investigation of neuroplastic changes in the hippocampus. Cellular models are frequently employed alone or in conjunction with animal studies to investigate the mechanisms of depression and antidepressants [ 19 ]. Hydrogen peroxide or glucocorticoids are often used to induce primary neuronal cell models (cortical or hippocampal neurons), neuronal cell lines (rat clonal pheochromocytoma PC12, human neuroblastoma SH-SY5Y, and mouse hippocampal HT-22), primary glial cell cultures (astrocytes, microglia), and glial cell lines (rat glioma C6, microglial BV2) to construct the cell model of MDD [ 19 , 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…Cellular models are frequently employed alone or in conjunction with animal studies to investigate the mechanisms of depression and antidepressants [ 19 ]. Hydrogen peroxide or glucocorticoids are often used to induce primary neuronal cell models (cortical or hippocampal neurons), neuronal cell lines (rat clonal pheochromocytoma PC12, human neuroblastoma SH-SY5Y, and mouse hippocampal HT-22), primary glial cell cultures (astrocytes, microglia), and glial cell lines (rat glioma C6, microglial BV2) to construct the cell model of MDD [ 19 , 20 ]. This study stimulated mouse hippocampal neuronal cell line (HT22 cell) with CORT and HG to simulate hippocampal neuron damage in the comorbidity of MDD and DM.…”

Section: Discussionmentioning

confidence: 99%

GLP-1 plays a protective role in hippocampal neuronal cells by activating cAMP-CREB-BDNF signaling pathway against CORT+HG-induced toxicity

Ma¹,

Wang²,

Liu³

et al. 2023

Heliyon

View full text Add to dashboard Cite

“…Hypericin's antioxidant, anti-inflammatory, and other pharmacological activities have been reported in the literature [47]. We could rely on the previously mentioned pharmacological activators of hypericin in reducing depression-like symptoms in mice, such as anti-inflammatory effect by inhibiting NO generation, reducing iNOS activity, and increasing the expression of heme oxygenase 1 (HO-1) [48]. This increased preference in sucrose was noted without any effect in the food intake (g) of animals in any of the groups (Figure 8B; p = 0.9314).…”

Section: Sucrose Preference Testmentioning

confidence: 99%

Production, Characterization, and In Vitro and In Vivo Studies of Nanoemulsions Containing St. John’s Wort Plant Constituents and Their Potential for the Treatment of Depression

et al. 2023

View full text Add to dashboard Cite

The current project was designed to prepare an oil-in-water (oil/water) hypericin nanoemulsion using eucalyptus oil for the preparation of an oil phase with chitosan as an emulsion stabilizer. The study might be a novelty in the field of pharmaceutical sciences, especially in the area of formulation development. Tween® 80 (Polysorbate) was used as the nonionic surfactant. The nanoemulsion was prepared by using the homogenization technique, followed by its physicochemical evaluation. The surface morphological studies showed the globular structure has a nano-sized diameter, as confirmed by zeta size analysis. The zeta potential analysis confirmed a positive surface charge that might be caused by the presence of chitosan in the formulation. The pH was in the range of 5.14 to 6.11, which could also be compatible with the range of nasal pH. The viscosity of the formulations was found to be affected by the concentration of chitosan (F1-11.61 to F4-49.28). The drug release studies showed that the presence of chitosan greatly influenced the drug release, as it was noticed that formulations having an elevated concentration of chitosan release lesser amounts of the drug. The persistent stress in the mouse model caused a variety of depressive- and anxiety-like behaviors that can be counteracted by chemicals isolated from plants, such as sulforaphane and tea polyphenols. In the behavioral test and source performance test, hypericin exhibited antidepressant-like effects. The results show that the mice treated for chronic mild stress had a considerably higher preference for sucrose after receiving continuous hypericin for 4 days (p = 0.0001) compared to the animals administered with normal saline (p ≤ 0.0001) as well as the naïve group (p ≤ 0.0001). In conclusion, prepared formulations were found to be stable and can be used as a potential candidate for the treatment of depression.

show abstract

Cell-Based Systems of Depression: An Overview

Cited by 6 publications

References 268 publications

Exploring the Role of Drug Repurposing in Bridging the Hypoxia–Depression Connection

Exploring the Role of Drug Repurposing in Bridging the Hypoxia–Depression Connection

GLP-1 plays a protective role in hippocampal neuronal cells by activating cAMP-CREB-BDNF signaling pathway against CORT+HG-induced toxicity

Production, Characterization, and In Vitro and In Vivo Studies of Nanoemulsions Containing St. John’s Wort Plant Constituents and Their Potential for the Treatment of Depression

Contact Info

Product

Resources

About