Cell Biological Analysis of DT40 Knockout Cell Lines for Cell‐Cycle Genes

Abstract

DT40 is a chicken B cell line that has been widely used as a model for gene functional studies because the high level of homologous recombination in DT40 cells allows targeted disruption of a gene of interest. While our laboratory uses DT40 cells to understand kinetochore assembly and function, the approach is applicable to functional studies of other genes that are required for cell cycle progression. Protocols are presented for the creation of knockout cells and subsequent cell biological analyses for characterizing the phenotypes of these cells. Curr. Protoc. Cell Biol. 50:8.7.1‐8.7.17. © 2011 by John Wiley & Sons, Inc.

Homologous Recombination and Translesion DNA Synthesis Play Critical Roles on Tolerating DNA Damage Caused by Trace Levels of Hexavalent Chromium

“…Because of the high level of homologous recombination and the relative ease of gene manipulation, DT40 cells, the chicken B-lymphocyte cells, have been widely used as a model system for higher vertebrate genetic functional studies [12, 13]. With the fast replication speed and the strong phenotypic similarities with murine cells, it makes the DT40 isogenic cell line and its mutants deficient in various genes ideal for reverse genetic studies [14].…”