Cell block sensitivity for immunohistochemical detection of cytokeratin 5, oestrogen and progesterone receptors in canine primary mammary carcinoma

Abstract

ABSTRACT. Mammary carcinomas are relatively common ailments among female canines aged around 10 years old, presenting an important morbidity with an average survival of five years. The cytoinclusion technique is frequently employed in human medicine as the investigative method of choice as it quickly provides resources for the determination of the correct therapeutic response, however, the effectiveness of the technique in canines remains understudied in veterinary medicine. This study aims at evaluating the degree of correlation with immunohistochemical marking for cytokeratin 5 (CK5), oestrogen and progesterone receptors (ER and PR) between the cytoinclusion and the histopathology technique in mammary carcinomas. Twenty-five samples of mammary carcinoma, both for the cytoinclusion and histopathological techniques were submitted for histological processing; microscope slides were created for hematoxylin-eosin (HE) staining and the immunohistochemical technique (IHC) was assessed for the ER, PR and CK5 receptors. Through the HE staining, we reached a concordance rate of 100% between the cytoinclusion and the histopathological analysis in the diagnosis of carcinomas. The immunohistochemical assay presented sensitivity of 85.71%, 95.45% and 100% and Cohen's kappa of 0.78, 0.84 and 0.95 for ER, PR and CK5, respectively, as well as 100% specificity and P<0.01 for all three markers. Therefore, cytoinclusion represents an accessible, fast and low-cost method, offering high sensitivity for the cytomorphological and immunohistochemical diagnosis of mammary carcinoma in female canines.Key words: cytoinclusion, histopathological, immunohistochemistry, tumor. RESUMEN.Los carcinomas mamarios son relativamente comunes en hembras caninas con edades de alrededor 10 años y representan un importante factor de morbilidad para estas hembras; la técnica de citoinclusión es frecuentemente empleada en medicina humana como un método de diagnóstico que permite proveer información rápida para la determinación de una conducta terapéutica correcta, sin embargo, la efectividad de esta técnica permanece poco estudiada en medicina veterinaria. El objetivo del estudio fue evaluar el grado de correlación entre marcadores inmunohistoquímicos para citokeratina 5 (CK5), receptores de estrógenos y progesterona (RE y RP) entre la técnica de citoinclusión y la técnica histopatológica en carcinoma mamario. Veinticinco muestras de carcinoma mamario para la técnica de citoinclusión e histopatológica fueron sometidas a procesamiento histológico, láminas fueron confeccionadas para la tinción mediante hematoxilina y eosina (HE), y la técnica de inmunohistoquímica para identificar la expresión de RE, RP y CK5. Mediante la tinción de la HE fue encontrado una proporción de concordancia del 100% entre el análisis de citoinclusión e histopatológico de carcinoma mamario. El análisis inmunohistoquímico evidenció una sensibilidad del 85,71%, 95,45% y 100% y un índice kappa de Cohen de 0,78, 0,84 y 0,95 para RE, RP y CK5, respectivamente, así como 100% de especifi...

Neuroendocrine Carcinomas of the Canine Mammary Gland: Histopathological and Immunohistochemical Characteristics

“…Reagents were manually applied and immunoreactivity was visualized by incubating the slides with diaminobenzidine chromogen (DAB Substrate System, Dako, Carpinteria, CA, USA) for 3 min. Details of the antibodies against Synaptophysin (1,22,23), NSE (22,24), CD56 (24), PGP 9.5 (25,26), Chromogranin A (25,27), Estrogen Receptor -RE (28), Progesterone Receptor -PR (29), Pancitokeratin (22) and Ki67 (30), dilutions, antigen retrieval procedures and incubation times used in the immunostaining process are shown in Table 1. Normal canine mammary gland was used as an internal positive control for Estrogen and Progesterone Receptors and Pancitokeratin.…”